Browsing by Author "Himanshu Pandey"

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CRISPR/Cas9 and Anther Culture for Precision Double Haploid Line Production in Controlled Environments
(John Wiley and Sons Inc, 2024) Avinash Sharma; Himanshu Pandey; Varucha Misra; Rajeev Kumar; Amit Vashishth; V.S. Devadas; A.K. Mall; Ashutosh; Megha Raghvan; Ajith Kumar Kesavan; Vishva Deepak Chaturvedi
The development of mapping populations and quantitative trait loci (QTL) analysis face constraints, in crops exhibiting male sterility and self-incompatibility under field conditions. Addressing these challenges requires the integration of advanced techniques, including the temporal alteration or excision of centromere histone H3 (CENH3) protein and the use of gene editing tools such as MATRILINEAL (MTL) knockout. Specifically, this can be achieved through Cas9/gRNA-mediated mutagenesis or Cas9/gRNA-driven promoter expression systems. These technologies offer efficient means to advance mapping populations and QTL analysis in male sterile and self-incompatible crops within controlled ecosystems. The doubled haploid (DH) mapping population, traditionally requiring 3 years of generation time via anther culture method, can now be expedited to 2–3 years of generation time using gene editing techniques within controlled environmental systems. Notably, DH mapping populations can be efficiently generated in various crops, including rice, wheat, maize, barley and oats by leveraging gene editing tools. Among these tools, the novel approach of CENH3 protein temporal alteration/excision emerges as highly efficient compared to MTL knockout using Cas9/gRNA-mediated mutation or Cas9/gRNA promoter expression. However, further investigation is warranted to optimise the regeneration of double haploid populations and enhance QTL analysis in male sterile and self-incompatible crops under controlled systems. © 2024 Wiley-VCH GmbH. Published by John Wiley & Sons Ltd.
Physiological, biochemical, and molecular adaptation mechanisms of photosynthesis and respiration under challenging environments
(Elsevier, 2021) Prajjal Dey; Diptanu Datta; Debasish Pattnaik; Deepali Dash; Debanjana Saha; Darshan Panda; Bishal Binaya Bhatta; Selukash Parida; Udit Nandan Mishra; Jyoti Chauhan; Himanshu Pandey; Rajesh Kumar Singhal
Photosynthesis and respiration are two central and basic physiological processes in regulation to carbon budget and carbon sink in the terrestrial ecosystem, as well as assessment and feedback of highly variable and fluctuating environmental conditions. Elevated CO2, salinity, alkalinity, drought, flood, nutrient deficiency and toxicity, extreme cold and heat, and various natural and anthropogenic pollutants are critical challenges in near future and their primary target is to alteration of photosynthetic and respirational processes, which ultimately influence the final yield potential of major agricultural crops. However, plants adapt themselves to these environmental circumstances via complicated changes at physiochemical and molecular levels. The current chapter highlights the underline mechanisms of photosynthetic and respiration in response to climate change at physiological, biochemical, and molecular levels. This chapter also covers the timely and substantial information regarding the recent progress in photosynthesis and respiration research. First, an outline of future climate change and its impacts on plant processes, functions, and yield potential is presented. Then, responses and adaptation of photosynthesis and respiration mechanisms against multiple stresses are discussed. Globally the present issues are crucial and this chapter helps in better understanding how plants deal with climatic change and their physiological, cellular, and molecular processes to the development of sustainable environment. © 2022 Elsevier Inc. All rights reserved.