Browsing by Author "Himanshu Tripathi"

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Enhanced bioactivity, biocompatibility and mechanical behavior of strontium substituted bioactive glasses
(Elsevier Ltd, 2016) Sampath Kumar Arepalli; Himanshu Tripathi; Sumit Kumar Hira; Partha Pratim Manna; Ram Pyare; S.P.Singh
Strontium contained biomaterials have been reported as a potential bioactive material for bone regeneration, as it reduces bone resorption and stimulates bone formation. In the present investigation, the bioactive glasses were designed to partially substitute SrO for SiO2 in Na2O–CaO–SrO–P2O5–SiO2 system. This work demonstrates that the substitution of SrO for SiO2 has got significant benefit than substitution for CaO in the bioactive glass. Bioactivity was assessed by the immersion of the samples in simulated body fluid for different intervals. The formation of hydroxy carbonate apatite layer was identified by X-ray diffractometry, scanning electron microscopy (SEM) and energy dispersive spectroscopy. The elastic modulus of the bioactive glasses was measured and found to increase with increasing SrO for SiO2. The blood compatibility of the samples was evaluated. In vitro cell culture studies of the samples were performed using human osteosarcoma U2-OS cell lines and found a significant improvement in cell viability and proliferation. The investigation showed enhancement in bioactivity, mechanical and biological properties of the strontia substituted for silica in glasses. Thus, these bioactive glasses would be highly potential for bone regeneration. © 2016
Enhanced in vivo biocompatibility of magnesia-contained bioactive glasses
(Springer International Publishing, 2019) Sampath Kumar Arepalli; Himanshu Tripathi; Partha Pratim Manna; Paliwal Pankaj; Sairam Krishnamurthy; Shashikant C. U. Patne; Ram Pyare; S.P. Singh
Human blood compatibility of previously prepared magnesia-doped bioactive glass samples was evaluated by hemolysis assay. The in vivo evaluation was carried out using a rat model in which a hole was made in the rat femur bone by drilling and the bioactive glass samples were implanted in the cavity. Histological examination had demonstrated the better bone regeneration and blood vessel formation as compared to the reference one. The in vivo complete blood count (CBC) was also analyzed at different time periods. The in vivo results of magnesium-containing bioactive glasses had shown improved bioactivity as well as better biocompatibility. © 2018, Australian Ceramic Society.
Pharmacological application of barium containing bioactive glass in gastro-duodenal ulcers
(Elsevier Ltd, 2018) Pankaj Paliwal; Arepalli Sampath Kumar; Himanshu Tripathi; S.P. Singh; Shashikant C.U. Patne; Sairam Krishnamurthy
Peptic ulcer is prevalent in about 4% of the world population and nearly 10% of people have been affected by peptic ulcer at some point in their life. Therefore, there is a need for newer efficient and safe anti-ulcer agents. In the present strategy, we have prepared a novel bioactive glass containing 1.3 mol% of barium oxide (BaBG) and evaluated its antiulcer potential in gastroduodenal ulcer models. Prophylactic effect of BaBG pretreatment was evaluated for 5 days in ethanol, aspirin and pyloric ligation-induced gastric ulcer and cysteamine-induced duodenal ulcer models. Repeated treatment of 10 days of BaBG was evaluated in the healing ulcer model of acetic acid. BaBG significantly reduced the ulcerative damage against all the five tested ulcer models. Scanning electron microscope (SEM) images have shown that BaBG forms a physical protective barrier over the gastro-duodenal epithelium cell. In the pyloric-ligation, ethanol and aspirin models, BaBG showed significantly increased in gastric pH, indicating antacid like activity. BaBG treatment significantly increased cell proliferation in the pyloric model. Thus, BaBG mediates antiulcer action by forming a protective physical barrier against harsh luminal factors, acid neutralization and cell proliferation. © 2018 Elsevier B.V.
Preparation and in vitro investigation on bioactivity of magnesia-contained bioactive glasses
(Springer International Publishing, 2019) Sampath Kumar Arepalli; Himanshu Tripathi; Partha Pratim Manna; Paliwal Pankaj; Sairam Krishnamurthy; Shashikant C.U. Patne; Ram Pyare; S.P. Singh
Mg plays an important role in the human skeletal system as it stimulates the bone formation and reduces bone resorption. Magnesium has been substituted into SiO 2 –Na 2 O–CaO–P 2 O 5 bioactive glass. In the present work, the bioactive glasses were designed to enhance bioactivity. Bioactivity of these glasses was assessed by the immersion of the samples in simulated body fluid (SBF) for different time periods. The formation of hydroxy carbonate apatite (HCA) layer was confirmed by scanning electron microscopy, X-ray diffractometry, and FTIR spectrometry which had shown the HCA layer formation and growth. The formation of HCA layer was found to increase on the surface of the SBF immersed bioactive glasses with increasing magnesia contents in the glass. In vitro cell culture investigations such as viability, proliferation, and cell attachment were studied using human osteosarcoma U2-OS cell lines. The in vitro results of new magnesium containing bioactive glasses had shown improved bioactivity as well as better biocompatibility. © 2018, Australian Ceramic Society.
Structural characterization and in vitro bioactivity assessment of SiO2–CaO–P2O5–K2O–Al2O3 glass as bioactive ceramic material
(Elsevier Ltd, 2015) Himanshu Tripathi; Sumit Kumar Hira; Arepalli Sampath Kumar; Uttam Gupta; Partha Pratim Manna; S.P. Singh
The potassium based bioactive glasses have shown a better biocompatibility than soda containing bioactive glasses. Therefore, we have prepared a bioactive glass system containing potassium oxide and substituted with Al2O3 for further enhancement of bioactivity, physico-chemical properties, mechanical strength as well as its behavior to human osteosarcoma cells. The prepared bioactive glasses have a general formula, 42SiO2–34CaO-6 P2O5–(18−x) K2O, where x=0, 0.5, 1, 1.5 and 2.5 mol% of Al2O3. The in-vitro bioactivity of these samples was assessed by immersion in SBF solution for different time periods under physiological conditions. The formation of hydroxy carbonate apatite (HCA) layer on the surface of the glass samples after SBF treatment was confirmed by FTIR, XRD and SEM. The partial substitution of Al2O3 for K2O in glass demonstrated a significant increase in mechanical properties such as compressive strength and elastic modulus, respectively. The cytotoxicity, cell viability, proliferation, apoptosis and cell attachment were assessed using human osteosarcoma U2-OS cell lines. The cell culture studies demonstrated that the samples containing high concentration of Al2O3 showed a cytotoxic nature against cell lines. But the blood compatibility showed that all the samples were tolerant. Finally, this study clearly concludes that the optimization of Al2O3 in present potash based bioactive glasses would be potential biomaterials for biomedical applications. © 2015 Elsevier Ltd and Techna Group S.r.l.
Structural, physico-mechanical and in-vitro bioactivity studies on SiO2–CaO–P2O5–SrO–Al2O3 bioactive glasses
(Elsevier Ltd, 2019) Himanshu Tripathi; Chandana Rath; Arepalli Sampath Kumar; Partha Pratim Manna; S.P. Singh
Strontium based bioactive glasses have shown a better biocompatibility than calcia based bioactive glasses. In this report, we have shown that the bioactivity is found to be even more when we incorporate Al2O3 upto 1.5 mol% in SiO2–CaO–P2O5–SrO bioactive glass. We have studied the structural, physico-mechanical and bioactive properties in these glasses with varying alumina concentration from 0.5 to 2.5 mol%. The bioactivity of the glasses is evaluated by in vitro test in simulated body fluid (SBF). The formation of hydroxy carbonated apatite layer (HCA) on the surface of glasses after immersion in SBF is identified by the XRD, FTIR and SEM. The substitution of Al2O3 for SrO in these glasses demonstrates a significant enhancement in compressive strength and elastic modulus. However cytotoxicity and cell viability assessed using human osteosarcoma U2-OS cell lines show the growth of the cells without causing any significant loss of viability and cell death upto 1.5 mol% addition of Al2O3. Osteosarcoma cells grow on the surface of bioglasses which make them biocompatible and fit for use in clinical trials. © 2018 Elsevier B.V.
Synthesis and characterization of barium-doped bioactive glass with potential anti-inflammatory activity
(Elsevier Ltd, 2021) Shreyasi Majumdar; Sumit Kumar Hira; Himanshu Tripathi; Arepalli Sampath Kumar; Partha Pratim Manna; S.P. Singh; Sairam Krishnamurthy
Bioactive glasses are mostly utilized as bone and dental implants. However, these biomaterials cause immunogenic response even in absence of immune stimulating signals. Therefore, there is a need to develop a biomaterial that would be immunologically inert and possesses beneficial biological effects depending upon the pathological condition. In this study, barium doped bioactive glass (BaBG) and 45S5 were synthesized in the nanometer range using the sol-gel process and the BET-specific surface area was assessed. The in-vitro bioactivity of BaBG and 45S5 was examined in the simulated body fluid (SBF). Hydroxyapatite crystals formed on the surface were evaluated using FTIR, X-ray diffractometry (XRD), scanning electron microscopy (SEM), energy-dispersive X-Ray spectroscopy (EDX), and pH changes of SBF. The doping of barium in bioactive glasses showed biocompatibility as the hemolytic index was within the permissible limit for biomedical agents. BaBG and 45S5 increased the percentage of cell proliferation without producing any cytotoxic effects on C6 and K562 cells. Moreover, BaBG showed coordinated horizontal cell movement in scratch assay which is a gold standard for the regeneration of injured tissues. Intriguingly, for the first time we reported that the incorporation of barium significantly reduced the LPS-induced elevation of interleukin-6 (IL-6) and tumor necrosis factor- α (TNF-α) in C6 cells. Simultaneously, BaBG also elevated IL-10 and hence showed anti-inflammatory properties. Therefore, based on the biocompatibility, cytocompatibility, and regenerative tendency, BaBG can be used for therapeutic purposes where inflammation restricts the use of biomaterials. © 2020