Browsing by Author "Indrani Nandi"

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Clinically Deployable Electro-Immunosensing Device Comprising Bioactive Glass-MWCNT for Alkaline Phosphatase Detection in Human Serum Samples
(American Chemical Society, 2025) None Darshna; Indrani Nandi; Pradeep Kumar Srivastava; Pranjal Chandra
Bone is a dynamic tissue that serves several purposes in the human body, including storing calcium, forming blood cells, and protecting and supporting the body’s organs. Alkaline phosphatase (ALP) is secreted into the circulation by osteoblasts, the cells responsible for making bone. It attaches to the surface of osteoblast cells or matrix vesicles. Accordingly, ALP is present in serum and is a popular biomarker for the evaluation of bone disease and other disorders in clinical evaluations. In this study, a composite probe made of bioactive glass (BG) and multiwalled carbon nanotubes (MWCNT) was used to detect ALP through electrochemical impedance spectroscopy (EIS) without the need for labels. By combination of physical methods with electrochemical analysis, the biosensor probe was characterized. The analytical performance of the biosensor was evaluated using EIS, and the results revealed that it has a limit of detection (LOD) of 2.29 ± 0.35 U/L and a linear dynamic range (LDR) of 25-600 U/L; both of which are within the acceptable range for ALP detection in clinics. When tested against serum interfering chemicals, the biosensor probe that was designed shows a strong selectivity for ALP (Ksel < 0.06). In addition, human serum and fetal bovine serum were used to test the system’s ability to detect ALP in biological samples. © 2025 American Chemical Society.
MOF-based nanocomposites as transduction matrices for optical and electrochemical sensing
(Elsevier B.V., 2024) Shubhangi; Indrani Nandi; S.K. Rai; Pranjal Chandra
Metal Organic Frameworks (MOFs), a class of crystalline microporous materials have been into research limelight lately due to their commendable physio-chemical properties and easy fabrication methods. They have enormous surface area which can be a working ground for innumerable molecule adhesions and site for potential sensor matrices. Their biocompatibility makes them valuable for in vitro detection systems but a compromised conductivity requires a lot of surface engineering of these molecules for their usage in electrochemical biosensors. However, they are not just restricted to a single type of transduction system rather can also be modified to achieve feat as optical (colorimetry, luminescence) and electro-luminescent biosensors. This review emphasizes on recent advancements in the area of MOF-based biosensors with focus on various MOF synthesis methods and their general properties along with selective attention to electrochemical, optical and opto-electrochemical hybrid biosensors. It also summarizes MOF-based biosensors for monitoring free radicals, metal ions, small molecules, macromolecules and cells in a wide range of real matrices. Extensive tables have been included for understanding recent trends in the field of MOF-composite probe fabrication. The article sums up the future scope of these materials in the field of biosensors and enlightens the reader with recent trends for future research scope. © 2023