Browsing by Author "Isha Sharma"

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Computational insights into CHST11 and CHST13: homology modeling, molecular docking, and dynamics simulation for novel therapeutic targeting in cancers
(Elsevier B.V., 2025) Isha Sharma; Vinay Kumar Singh; Rajan Vyas; Malkhey Verma
Hepatocellular carcinoma (HCC) is the most common primary liver malignancy and remains a major global health concern due to its high mortality and resistance to current therapies. Emerging evidence highlights the role of carbohydrate sulfotransferases CHST11 and CHST13 in driving tumor progression, activating MAPK signaling, and mediating chemoresistance. In this study, homology modeling, molecular docking, and molecular dynamics (MD) simulations were used to explore the structural and functional properties of CHST11 and CHST13, key sulfotransferases implicated in HCC. Active site analysis and interaction profiling guided the screening of 60 bioactive compounds, with ascorbic acid as a reference. Capsaicin bound strongly to both CHST11 and CHST13, whereas erlotinib exhibited selective affinity for CHST13. Notably, the CHST13-capsaicin complex demonstrated the most favorable binding energy and structural stability in MD simulations. These findings highlight the distinct dynamic behaviors of both targets and support their potential as druggable proteins in HCC, offering a basis for developing selective therapeutic inhibitors. © 2025
Development of chloroplast derived SSR markers for genus Allium and their characterization in the allies for genetic improvement of Alliums
(Elsevier B.V., 2023) Kuldip Jayaswall; Himanshu Sharma; Deepanshu Jayaswal; R. Sagar; Abhishek Bhandawat; Akash Kumar; Isha Sharma; Pratibha Chaturvedi; Dalasanuru Chandregowda Manjunathagowda; Deepesh Kumar; Vijay Mahajan; Sanjay Kumar; Major Singh
Alliums are the most popular for their culinary usage and nutraceutical benefits. Their production is greatly affected by the multiple biotic and abiotic stresses. Poor characterizations of genetic resources are the major bottleneck in the genetic improvement of Alliums. Chloroplast-derived simple sequence repeat has recently gained much popularity due to their maternal inheritance and low recombination along with their hypervariable nature. In this study, 22 of the 28 chloroplast-derived markers from chloroplast genomes of A. cepa and A. sativum were reported as polymorphic and cross-transferable. Tetra nucleotide repeats were more prevalent (52 %) in these 22 cpSSR markers as compared to other repeat motifs. Further polymorphic information content of these 22cpSSR markers ranged from 0.007 to 0.427. Based on the genotyping of 22cpSSR markers, 25 Alliums were clustered into two groups (groups I, IIA, and IIB). This showed that cultivated Allium cepa and Allium sativum belong to group II rather than the majority of wild Alliums, which belong to group I. Hence This proved the widespread use of these AccpSSR and AscpSSR markers. Additionally, it was clear from 79 Alliums morphological and genotyping-based clustering that these 79 wild Alliums exhibit strong clustering consistency in genotyping and phenotyping-based dendrograms, hence confirming these cpSSR's discrimination potential. Allium tuberosum Rottler ex. Spreng is quite distinct from Allium fistulosum L, A. schoenoprasumas as well as Allium cepa var. aggregatum G.Don. Further Allium fistulosum L, A. schoenoprasum and Allium cepa var. aggregatum are closely related, according to genotyping-based dendrograms of 79 wild Alliums. Hence Allium fistulosum L, A. schoenoprasum, and Allium cepa provide a brilliant opportunity for introgression of the high total soluble content, high quercetin content, and allicin content of cultivated Allium into wild Allium as well as the biotic and abiotic stress tolerance of wild Alliums into cultivated Alliums. Subsequent clustering of 79 Alliums further allowed us to identify diverse Alliums for constructing a core collection. Hence present study will be useful for molecular breeding and genomic selection-based crop improvement. © 2023 SAAB
Formation of solar spicules due to high-frequency perturbation in the solar photosphere
(Elsevier Ltd, 2025) Isha Sharma; Disha Varshney; Kedar A.P. Singh
Spicules are jet-like structures, visible on the Sun and noticed quite frequently during space-based observations of the solar chromosphere. It is important to understand the physical processes that could lead to the formation of taller spicules having heights larger than 6 Mm. In the present work, we have provided high-frequency photospheric perturbation and studied nonlinear processes in the spicule formation under the framework of magnetohydrodynamics (MHD). Perturbations in the solar photosphere include pressure perturbations and torsional velocity perturbations. The solar spicules in our model are formed due to the interaction of nonlinear MHD waves, specifically slow magneto-acoustic and Alfvén waves with the solar transition region. Through a self-consistent approach and 1.5D MHD simulations, we found that the high-frequency perturbation in the solar photosphere could generate taller spicules if a suitable wave period and height of the solar transition region are considered initially. The simulations are performed over a long time (e.g., up to 800 min), and in certain conditions, we found that the spicule reaches a height of 10 Mm and beyond. Furthermore, high-frequency perturbations play a significant role in forming the spicules and chromospheric heating. © 2025 COSPAR
Immunomodulator and marker-assisted identification of Allium genotypes containing immunological memory against anthracnose
(Academic Press, 2025) Kuldip Jayaswall; Deepesh Kumar; Deepanshu Jayaswal; Ram Sagar; Sanjay Kumar; Ram Kumar Sharma; Kiran Pandurang Bhagat; Vijay M. Mahajan; Himanshu Sharma; Surendra Pratap Singh; Isha Sharma
Allium cepa (onion) suffers significant yield losses due to anthracnose disease caused by Colletotrichum gloeosporioides. Current control methods, such as biocontrol agents, have limited effectiveness, while agrochemical applications pose risks to human health and the environment. Genomics-assisted breeding can be useful in getting a diverse genetic pool of wild Allium species to improve disease resistance in cultivated varieties. In this study, we used 42 intron length polymorphic and 22 chloroplast-based SSR markers to identify wild Alliums for anthracnose resistance. Genetic analysis using the marker data showed that two wild types, 6AfistAKO-17 and 15Afistul, are very different from the cultivated types. Immunomodulator benzo-thiadiazole-7-carbothioic acid S-methyl ester evoked a long-lasting immune response in wild Alliums. Transcriptome profiling showed that 131 immune-related genes were more than twice as active in wild Alliums compared to cultivated ones. These included MAPKs (7 genes), WRKY transcription factors (10), R genes (19), MYB transcription factors (28), cytochrome P450s (46), transcriptional activators (14), and other immune-associated genes (7). The results from the anatomical analysis showed that wild Alliums have more callose in their vascular bundles, thicker wax on their leaf surfaces, and closed stomata, which were confirmed by fluorescence and scanning electron microscopy, indicating that wild Alliums have a stronger immune system than the cultivated Alliums. Our findings suggest that wild Alliums possess both immunity and immune memory-related genes. This study suggests that molecular markers could help transfer the immunity-related genes from wild Alliums to cultivated Alliums to protect them from anthracnose infestation. © 2025 Elsevier Ltd
MEASUREMENT OF EXCITATION FUNCTION IN THE 16 O + 107Ag SYSTEM AT ENERGIES ABOVE THE COULOMB BARRIER
(National Academy of Sciences of Ukraine, 2025) Diwanshu; Ajay Vinod Kumar; Bharti Rohila; Chetan Kumar Sharma; Subodh; P. S. Rawat; Amit Kumar Pandey; Kaushik T. Katre; Honey Arora; U. S. Ghosh; Yashraj; Isha Sharma; Hariprakash P. Sharma; S. K. Chamoli; S. Dasaratha Kumar; R. P. Singh; Sivaramakrishnan Muralithar
The decay mechanism of the highly excited compound nucleus123Cs populated via fusion evaporation reaction of 16 O and107Ag is studied. De-excitation of the compound nucleus via evaporation of p, n, and α-particles leads to a population of several neutron-deficient residual nuclei. The excitation function for the16O +107Ag reaction has been determined experimentally in the energy range 71-80 MeV above the Coulomb barrier. The experimental results have been analyzed within the framework of statistical codes PACE4 and CASCADE. © The Author(s), 2025.