Browsing by Author "Jai Prakash Srivastava"

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Antioxidative defense system in pigeonpea roots under waterlogging stress
(Polish Academy of Sciences, Institute of Slavic Studies, 2012) Ruchi Bansal; Jai Prakash Srivastava
Pigeonpea [Cajanus cajan (L.) Millsp.] is a waterlogging-sensitive legume crop. We studied the effect of waterlogging stress on hydrogen peroxide (H2O2) content, lipid peroxidation and antioxidant enzyme activities in two pigeonpea genotypes viz., ICPL-84023 (waterlogging resistant) and MAL-18 (waterlogging susceptible). In a pot experiment, waterlogging stress was imposed for 6 days at early vegetative stage (20 days after sowing). Waterlogging treatment significantly increased hydrogen peroxide accumulation and lipid peroxidation, which indicated the extent of oxidative injury posed by stress conditions. Enzyme activities of peroxidase (POX), catalase (CAT), ascorbate peroxidase (APX), superoxide dismutase (SOD) and polyphenol oxidase (PPO) increased in pigeonpea roots as a consequence of waterlogged conditions, and all the enzyme activities were significantly higher in waterlogged ICPL-84023 than in MAL-18. POX activity was the maximum immediately after imposing stress, therefore, it was suggested to be involved in early scavenging of H2O2, while rest of the enzymes (CAT, APX, SOD and PPO) were more important in late responses to waterlogging. Present study revealed that H2O2 content is directly related to lipid peroxidation leading to oxidative damage during waterlogging in pigeonpea. Higher antioxidant potential in ICPL-84023 as evidenced by enhanced POX, CAT, APX, SOD and PPO activities increased capacity for reactive oxygen species (ROS) scavenging and indicated relationship between waterlogging resistance and antioxidant defense system in pigeonpea. © 2011 Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków.
Antioxidative responses to short term waterlogging stress in pigeon pea
(Springer Verlag, 2015) Ruchi Bansal; Jai Prakash Srivastava
Waterlogging stress is a major abiotic stress having detrimental effects on crop production. Pigeon pea (Cajanus cajan (L.) Millsp.) is a waterlogging sensitive legume crop. Effect of short term waterlogging stress was studied on the activity of antioxidant enzymes in two pigeon pea genotypes, viz., ICPL-84023 (waterlogging resistant) and MAL-18 (waterlogging susceptible). The objective of the present experiment was to study the changes in antioxidant enzyme system in response to waterlogging. Waterlogging stress was imposed for 12 h at early vegetative stage (20 days after sowing), and activities of superoxide dismutase, ascorbate peroxidase, catalase, peroxidase and polyphenol oxidase were recorded in the root tissues at 0, 3, 6, 9 and 12 h after imposing waterlogging stress. Enzyme activities were higher in ICPL-84023 as compared to MAL-18. Activities of peroxidase and polyphenol oxidase increased immediately (3 h) after imposing waterlogging stress, indicating the activation of antioxidant enzyme system at very early stages. Changes in superoxide dismutase activity were variable at different stages of observation. Catalase activity increased significantly at 9 h and then decreased. Ascorbate peroxidase activities did not change significantly under waterlogging. © 2015, Indian Society for Plant Physiology.
Effect of waterlogging on root anatomy and nitrogen distribution in pigeonpea (Cajanus cajan (L.) Millsp.)
(Springer Verlag, 2017) Ruchi Bansal; Jai Prakash Srivastava
We studied the effect of waterlogging on root anatomical changes and nitrogen content in pigeonpea, a waterlogging sensitive crop. A pot experiment was conducted with pigeonpea genotypes; ICPL-84023 (waterlogging tolerant) and MAL-18 (waterlogging susceptible). Stress was imposed after 20 days of sowing for 6 days. Aerenchyma development was observed in genotype ICPL-84023 and aerenchyma occupied 12 % root cortical area after 6 days of imposing stress. No change in root anatomy was observed in MAL-18 under waterlogging. Root nitrogen content reduced 40 % in ICPL-84023 (2.47 mg g−1 dry weight) and 51.9 % in MAL-18 (2.06 mg g−1 dry weight) compared to control after 6 days of waterlogging. In stem, nitrogen content was 1.54 and 1.26 mg g−1 dry weight in ICPL-84023 and MAL-18, respectively, after 6 days of stress. Lower leaves abscessed in MAL-18 after 4 days, while, in upper leaves, nitrogen content decreased by 20.4 % in ICPL-84023 and 88.8 % in MAL-18. Waterlogging affected nitrogen uptake and partitioning adversely in both the genotypes. But the ability to maintain high nitrogen content under stress to sustain growth as evident by leaf retention was observed in ICPL-84023. Aerenchyma development is a well known survival strategy facilitating gaseous movement under waterlogging. It was concluded that aerenchyma formation and high nitrogen uptake play an important role in waterlogging tolerance in pigeonpea. © 2016, Indian Society for Plant Physiology.
Residual Effect of Boron and Sodium Chloride Applied to Wheat on Growth and Biochemical Parameters of Green Gram (Vigna radiata) Grown in Subsequent Season
(Agricultural Research Communication Centre, 2025) Ramachandra Abhishree; Jai Prakash Srivastava; Uday Pratap Singh; Jyostnarani Pradhan
Background: Experiment was conducted in pots to examine the residual effects of different levels of soil applied boron and sodium chloride (NaCl) to wheat during rabi on growth and biochemical parameters of green gram sown in following kharif. Methods: Boron or NaCl stress, which were applied individually or in combinations to wheat, had significant residual effects on biochemical processes and yield attributes of green gram. Result: At rising levels of NaCl or B, the content of sugar, starch, as well as free amino acids in leaves decreased, while the content of proline was increased. Under boron and NaCl stress, the MDA level increased dramatically due to lipid peroxidation. There were noteworthy residual toxic effects on growth, development, biochemical parameters, yield and yield attributes of green gram sown in the following kharif season. © 2025, Agricultural Research Communication Centre. All rights reserved.