Browsing by Author "Jitendra Kumar Chaurasia"

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Antioxidant property of aerial parts and root of phyllanthus fraternus webster, an important medicinal plant
(2014) Richa Upadhyay; Jitendra Kumar Chaurasia; Kavindra Nath Tiwari; Karuna Singh
In present study free radical scavenging potential of aerial parts and root of Phyllanthus fraternus was investigated. Extraction was done in water and ethanol. Total antioxidant capacity was measured by DPPH free radical scavenging method; ethanolic extract of aerial part was most potent in activity with 50% inhibition at 258 μg/mL concentration. Lipid peroxidation (LPO) was measured in terms of thiobarbituric acid-reactive substances (TBARS) by using egg-yolk homogenates as lipid-rich media with ECof aerial part (ethanolic) 1522 μg/mL which was found to be most active. Superoxide (SO) radical scavenging activity was measured using riboflavin-light-nitroblue tetrazolium assay. Ethanolic and aqueous extract of both aerial part and root was almost similar in superoxide radical scavenging activity. Reducing power was determined on the basis of Fe 3 + - Fe 2 + transformation in the presence of extract. Total phenolic and flavonoid contents were also measured by spectroscopic method. Results showed that the ethanolic fraction of aerial part is most active towards antioxidant potential and this activity is related to its polyphenolic content and reducing potential. Thus, P. fraternus extract can be used as potent natural antioxidant. © 2014 Richa Upadhyay et al.
Assessment of antioxidant potential in seed extracts of Nyctanthes arbor-tristis L. and phytochemical profiling by Gas Chromatography-Mass Spectrometry system
(Faculdade de Ciencias Farmaceuticas (Biblioteca), 2022) Awadhesh Kumar Mishra; Kavindra Nath Tiwari; Rajesh Saini; Jitendra Kumar Chaurasia; Sunil Kumar Mishra
The present study has been carried out with the seed extracts of Nyctanthes arbor-tristis L. (Parijat) and evaluates its antioxidant potential and profiling the phytochemical constituents by Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The antioxidant potential of the seed extracts was measured by four different in vitro assay like 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, superoxide anion free radical scavenging activity, ferric reducing antioxidant power (FRAP) and lipid peroxidation inhibition potential (LPIP) assay. The total phenol content (TPC) and total flavonoid content (TFC) were estimated. The ethyl acetate extract (EAE) of seeds showed potential DPPH free radical scavenging activity (EC50 129.49±3.55µg/ml), superoxide anion radical (EC50 969.94±8.03µg/ml) and LPIP (EC50 452.43±5.07 µg/ml) activities. The total phenol content was maximum in aqueous extract (AQE) which was 201.00±0.20 µg/mg gallic acid equivalent. The EAE was rich with total flavonoid and it was found to be 34.50±0.40 µg/mg rutin equivalent. The EAE was subjected for phytochemical-profiling using GC-MS system. The presence of different phytoconstituents supports the medicinal value of the seeds. The results suggest that EAE constitutes a promising new source of novel compounds. Further, it can be used for isolation and purification of specific compounds which have good antioxidant activities and possess useful biological activities. © 2022, Faculdade de Ciencias Farmaceuticas (Biblioteca). All rights reserved.
Comparative antioxidant study in different flower extracts of Nyctanthes arbor-tristis (L.) (Oleaceae): an important medicinal plant
(Springer International Publishing, 2016) Awadhesh Kumar Mishra; Richa Upadhyay; Jitendra Kumar Chaurasia; Kavindra Nath Tiwari
Nyctanthes arbor-tristis (L.) popularly known as ‘Parijat’ belongs to the family Oleaceae and is widely used in the traditional system of medicines in India. It has immense biological properties. In the present study, the shade-dried flowers were extracted in three different solvents such as ethanol, ethyl acetate, and water. The antioxidant activities were evaluated by four different in vitro assays—DPPH free radical scavenging, superoxide radical scavenging, lipid peroxidation, and reducing power determination. Phytoconstituents like total phenolic and flavonoid content of the extracts were also measured. The maximum antioxidant activity was observed in ethanolic extract of the flower. The higher phenolic content was found in aqueous extract (177.0 ± 0.17 µg mg−1 gallic acid equivalent). Flavonoid content was higher in ethanolic extract 29.25 ± 0.13 µg mg−1 equivalent to rutin) of flower than the other two extracts. This indicates that both phenolic and flavonoid contents might be responsible for the antioxidant potential of the flower. Ethanolic extract of the flower was found most potent in antioxidant activity in all assays. Thus, the ethanolic extract of the flower can be used as a cheap natural source of antioxidants. © 2016, Botanical Society of Sao Paulo.
Comparative antioxidant study of stem and stem induced callus of Phyllanthus fraternus webster - An important antiviral and hepatoprotective plant
(2013) Richa Upadhyay; Jitendra Kumar Chaurasia; Kavindra Nath Tiwari; Karuna Singh
Phyllanthus fraternus is widely used in the cure of various liver diseases and possess antiviral properties especially against hepatitis virus. In the present study, evaluation of the antioxidant activity of stem and calli induced from stem has been done by different assays. Extraction was done by standard method in water and ethanol. Total antioxidant capacity was measured by 1, 1-diphenyl-2-picrylhydrazyl free radical scavenging method. Lipid peroxidation was measured in terms of thiobarbituric acid-reactive substances (TBARS) by using egg yolk homogenates as lipid-rich media, and superoxide radical scavenging activity was measured using riboflavin-light-nitro blue tetrazolium assay. Reducing power was determined on the basis of Fe3+-Fe 2+ transformation in the presence of the extract. In addition to the antioxidant activity, polyphenolic compounds like total phenolics and flavonoids were also measured by spectroscopic method. Results showed that the ethanolic extract of stem is more potent in antioxidant activity than its aqueous extract and ethanolic extract of calli. A significant correlation between antioxidant capacity and polyphenolic content and reducing potential was observed, indicating that phenolic compounds and reducers present in extract are major contributors to the antioxidant potential. Thus, this plant extract could be used as a potent natural antioxidant. © Springer Science+Business Media New York 2013.