Browsing by Author "Jyotsana Pandey"

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Comparative evaluation of liquid-liquid extraction and nanosorbent extraction for HPLC-PDA analysis of cabazitaxel from rat plasma
(Elsevier B.V., 2024) Medapati Nikitha Lakshmi Suseela; Abhishesh Kumar Mehata; Bhaskar Vallamkonda; Pathraj Gokul; Aditi Pradhan; Jyotsana Pandey; Joseph Selvin; M. Sterlin Leo Hudson; Madaswamy S. Muthu
A precise, sensitive, accurate, and validated reverse-phase high-performance liquid chromatography (RP-HPLC) method with a bioanalytical approach was utilized to analyze Cabazitaxel (CBZ) in rat plasma. Comparative research on extraction recoveries was performed between traditional liquid-liquid extraction (LLE) and synthesized graphene oxide (GO) based magnetic solid phase extraction (GO@MSPE). The superparamagnetic hybrid nanosorbent was synthesized using the combination of iron oxide and GO and subsequently applied for extraction and bioanalytical quantification of CBZ from plasma by (HPLC-PDA) analysis. Fourier- transform infrared spectroscopy (FT-IR), particle size, scanning electron microscopy (SEM), and x-ray diffraction (XRD) analysis were employed in the characterization of synthesized GO@MSPE nanosorbent. The investigation was accomplished using a shim pack C18 column (150 mm×4.6 mm, 5 µm) with a binary gradient mobile phase consisting of formic acid: acetonitrile: water (0.1:75:25, v/v/v) at a 0.8 mL/min flow rate, and a λmax of 229 nm. The limits of detection (LOD) and quantitation (LOQ) have been determined to be 50 and 100 ng/mL for both LLE and SPE techniques. The linearity range of the approach encompassed from 100 to 5000 ng/mL and was found to be linear (coefficient of determination > 0.99) for CBZ. The proposed method showed extraction recovery of 76.8–88.4% for the synthesized GO@MSPE and 69.3–77.4% for LLE, suggesting that the proposed bioanalytical approach was robust and qualified for all validation parameters within the acceptable criteria. Furthermore, the developed hybrid GO@MSPE nanosorbent with the help of the proposed RP-HPLC method, showed a significant potential for the extraction of CBZ in bioanalysis. © 2024 Elsevier B.V.
Electrochemical capacitance and hydrogen adsorption behavior of activated carbon derived from cattail fiber
(John Wiley and Sons Inc, 2024) Ramesh Achayalingam; Sourabh Basu; Prins Kumar Rao; Jyotsana Pandey; Nivetha Selvaraj; Jayachitra Selvam; M. Sterlin Leo Hudson
In this paper, we have reported the synthesis of activated carbon (AC) from biomass cattail fiber through hydrothermal carbonization, followed by chemical activation, and its electrochemical capacitance and hydrogen storage properties. The AC exhibits a Brunauer-Emmett-Teller (BET) surface area (SBET) of 1597.5 m2g−1, determined from the low-pressure N2 adsorption isotherm at 77 K using a BET-multipoint plot. The AC sample shows a reversible hydrogen adsorption capacity of 0.25 wt.% H2 (1.25 mmol H2 g−1) at 293 K and 74 atm. The capacitance performance of AC was investigated with various conductive additives such as carbon nanotubes (CNTs), carbon black (CB), and reduced graphene-oxide (rGO). From galvanostatic charge discharge (GCD) and cyclic voltammetry (CV) measurements, the as-derived AC with polymer binder exhibits a specific capacitance (Cs) of 245.2 F g−1 at 0.2 A g−1 and 158.1 F g−1 at 5 mV s−1. Among the investigated conductive additives, AC with CNTs in KOH electrolyte exhibit highest Cs of 326 F g−1 at 0.2 A g−1 and 173 F g−1 at 5 mV s−1. Furthermore, the symmetrical two-electrode device fabricated using AC with CNTs (as a conductive additive) in 1 M aq. Na2SO4 electrolyte shows a Cs of 97.2 F g−1 at 0.1 A g−1. The energy and power densities of the two-electrode device were observed to be 28 kW kg−1 and 2.64 Wh kg−1, respectively. © 2024 John Wiley & Sons Ltd.
Improved electrocatalytic performance of delaminated-MXene and cobalt ferrite nanocomposite for hydrogen evolution in acidic medium
(Elsevier Ltd, 2025) Jyotsana Pandey; Sourabh Basu; Shalinee Dubey; Vellaichamy Ganesan; Mohammad Abu Shaz; M. Sterlin Leo Hudson
Recently, the electrochemical hydrogen evolution reaction (HER) for hydrogen generation has garnered research attention due to growing environmental concerns over fossil fuels usage. This paper discusses HER activity of cobalt ferrite and delaminated-MXene (D-MX) nanocomposites at different mass ratios. The electrochemical performance of the nanocomposites was evaluated based on their structural, microstructural, and spectroscopic features. D-MX was successfully synthesized from the MAX phase by the chemical etching route, followed by a chemical delamination process. CoFe2O4 nanoparticles (CF) were synthesized using the sol-gel auto-combustion method. It has been observed that the D-MX and CF nanocomposite with the mass ratio of 5:1 (CMX51) exhibits superior electrocatalytic behavior for HER. When compared to other mass ratios, the composite CMX51 has the lowest overpotential of 681 mV in a 0.5 M acidic solution. The CMX51 composite demonstrated a Tafel slope of 112 mV/dec for the hydrogen evolution reaction (HER), which is lower compared to that of CF. This indicates that the presence of D-MX in the nanocomposite improved the sluggish kinetic behavior of the CF nanoparticles. This may be attributed to the enhancement in ionic conductivity of CF with improved charge transfer kinetics resulting from the addition of D-MX. The Electrochemical impedance spectroscopy analysis reveals that the nanocomposite CMX51 shows improved ionic conductivity with a low charge transfer resistance. © 2025
Tinosporaside from Tinospora cordifolia Encourages Skeletal Muscle Glucose Transport through Both PI-3-Kinase- and AMPK-Dependent Mechanisms
(MDPI, 2023) Akansha Mishra; Khushbu Sharma; Jyotsana Pandey; Kapil Dev; Sleman Kadan; Mahendra Sahai; Ishbal Ahmad; Arvind K. Srivastava; Akhilesh K. Tamrakar; Hilal Zaid; Rakesh Maurya
The stem of Tinospora cordifolia has been traditionally used in traditional Indian systems of medicine for blood sugar control, without the knowledge of the underlying mechanism and chemical constitution responsible for the observed anti-diabetic effect. In the present study, Tinosporaside, a diterpenoid isolated from the stem of T. cordifolia, was investigated for its effects on glucose utilization in skeletal muscle cells, which was followed by determining the anti-hyperglycemic efficacy in our diabetic db/db mice model. We found that tinosporaside augmented glucose uptake by increasing the translocation of GLUT4 to the plasma membrane in L6 myotubes, upon prolonged exposure for 16 h. Moreover, tinosporaside treatment significantly increased the phosphorylation of protein kinase B/AKT (Ser-473) and 5′ AMP-activated protein kinase (AMPK, Thr-172). These effects were abolished in the presence of the wortmannin and compound C. Administration of tinosporaside to db/db mice improved glucose tolerance and peripheral insulin sensitivity associated with increased gene expression and phosphorylation of the markers of phosphoinositide 3-kinases (PI3Ks) and AMPK signaling in skeletal muscle tissue. The findings revealed that tinosporaside exerted its antidiabetic efficacy by enhancing the rate of glucose utilization in skeletal muscle, mediated by PI3K- and AMPK-dependent signaling mechanisms. © 2023 by the authors.