Browsing by Author "K. Sairam"

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Anti-ulcer drugs from indigenous sources with emphasis on Musa sapientum, Tamrabhasma, Asparagus racemosus and Zingiber officinale
(2002) R.K. Goel; K. Sairam
Sula, Parinamasula and Amlapitta are clinical entities recognized by ayurveda, akin to peptic ulcer and functional dyspepsia. Many indigenous drugs have been advocated in ayurveda for treatment of dyspepsia. Our laboratory has been engaged in screening of various indigenous herbal and metallic drugs for their potential use in peptic ulcer diseases, taking lead from Ayurveda and have reported anti-ulcer and ulcer-healing properties of Tectona grandis (lapachol), Rhamnus procumbens (kaempferol), Rhamnus triquerta (emodin), Withania somnifera (acylsteryl glycoside), Shilajit (fulvic acid and carboxymethoxybiphenyl), Datura fastuosa (withafastuosin E), Fluggea microcarpa and Aegle marmelos (pyrano- and iso- coumarins) etc., along with their mechanism of action. The present article includes the detailed exploration of ulcer protective and healing effects of unripe plantain banana, tambrabhasma and Asparagus racemosus on various models of experimental gastroduodenal ulceration and patients with peptic ulcer. Their effects on mucin secretion, mucosal cell shedding, cell proliferation, anti-oxidant activity, glycoproteins, and PG synthesis have been reported. Clinical trials of these drugs for evaluating their potential ulcer healing effects in peptic ulcer patients have been done. Their potential ulcer protective effects both, experimental and clinical seemed to be due to their predominant effects on various mucosal defensive factors rather than on the offensive acid-pepsin secretion. Thus, the above herbal / herbo-mineral drugs do have potential usefulness for treatment of peptic ulcer diseases.
Antidepressant activity of standardized extract of Bacopa monniera in experimental models of depression in rats
(Urban und Fischer Verlag Jena, 2002) K. Sairam; M. Dorababu; R.K. Goel; S.K. Bhattacharya
Bacopa monniera Wettst. (syn. Herpestis monniera L.; Scrophulariaceae) is a commonly used Ayurvedic drug for mental disorders. The standardized extract was reported earlier to have significant anti-oxidant effect, anxiolytic activity and improve memory retention in Alzheimer's disease. Presently, the standardized methanolic extract of Bacopa monniera (bacoside A - 38.0 ± 0.9) was investigated for potential antidepressant activity in rodent models of depression. The effect was compared with the standard antidepressant drug imipramine (15 mg/kg, ip). The extract when given in the dose of 20 and 40 mg/kg, orally once daily for 5 days was found to have significant antidepressant activity in forced swim and learned helplessness models of depression and was comparable to that of imipramine.
Antiulcerogenic activity of Satavari mandur-An ayurvedic herbo-mineral preparation
(2002) G.K. Datta; K. Sairam; S. Priyambada; P.K. Debnath; R.K. Goel
Satavari mandur (SM) is a herbo-mineral preparation containing Asparagus racemosus, which finds mention in ancient Indian texts for treatment of gastric ulcers. The ulcer protective effect of SM, 125-500 mg/kg given orally, twice daily for three, five and seven days, was studied on cold restraint stress-induced gastric ulcer in rats. The effective regimen was found to be 250 mg/kg given for five days and hence was used for further experiments. SM showed significant protection against acute gastric ulcers induced by pyloric ligation but was ineffective against aspirin- and ethanol-induced ulcers. Further, gastric juice studies showed that, SM significantly increased the mucosal defensive factors like mucus secretion, but had little of no effect on offensive factors like acid and pepsin secretion.
Antiulcerogenic effect of methanolic extract of Emblica officinalis: An experimental study
(2002) K. Sairam; Ch.V. Rao; M.Dora Babu; K.Vijay Kumar; V.K. Agrawal; R. K. Goel
The ulcer protective potential of methanolic extract of Emblica officinalis Gaertn. (EOE) was assessed in different acute gastric ulcer models in rats induced by aspirin, ethanol, cold restraint stress and pyloric ligation and healing effect in chronic gastric ulcers induced by acetic acid in rats. EOE, 10-50 mg/kg administered orally, twice daily for 5 days showed dose-dependent ulcer protective effects in all the above acute ulcer models (36.0-98.3% protection, P<0.2 to P<0.001) and significant ulcer healing effect in dose of 20 mg/kg after 5 (control ulcer index: 20.2±2.3 mm2/rat, % healing 59.6%, P<0.001) and 10 (control UI: 11.0±1.7, % healing 65.5%, P<0.01) days treatment. Further study on gastric mucosal factors showed that it significantly decreased the offensive factors like acid (acid output-control 118.7±12.1 μEq/4 h, EOE% decrease 65.9%, P<0.01) and pepsin (peptic output-control 738.8 μmol/4 h, EOE% decrease 46.2%, P<0.001) and increased the defensive factors like mucin secretion (TC:P ratio-control 1.21±0.15, EOE% increase 95.0%, P<0.01), cellular mucus (TC:P ratio-control 1.16±0.13, EOE% increase 53.4%, P<0.05) and life span of mucosal cells (DNA content of gastric juice-control 77.3±8.7 μg/m per 100 g body weight, EOE% decrease 42.1%, P<0.05). EOE showed significant antioxidant effect in stressed animals (control UI 35.8±2.5, antioxidant status: LPO 0.58±0.03 nmol MDA/mg protein, SOD and CAT 227.8±6.3 and 18.4±1.2 U/mg protein respectively; EOE% decrease in UI 88.2%, mucosal LPO 69.0%, SOD 53.1% and increase in mucosal CAT 59.8%, P<0.001 respectively) and did not have any effect on cell proliferation in terms of DNA μg/mg protein or glandular weight. The results showed that EOE had significant ulcer protective and healing effects and this might be due to its effects both on offensive and defensive mucosal factors. © 2002 Published by Elsevier Science Ireland Ltd.
Anxiolytic-antidepressant activity of Withania somnifera glycowithanolides: An experimental study
(2000) S.K. Bhattacharya; A. Bhattacharya; K. Sairam; S. Ghosal
The roots of Withania somnifera (WS) are used extensively in Ayurveda, the classical Indian system of medicine, and WS is categorized as a rasayana, which are used to promote physical and mental health, to provide defence against disease and adverse environmental factors and to arrest the aging process. WS has been used to stabilize mood in patients with behavioural disturbances. The present study investigated the anxiolytic and antidepressant actions of the bioactive glycowithanolides (WSG), isolated from WS roots, in rats. WSG (20 and 50 mg/kg) was administered orally once daily for 5 days and the results were compared by those elicited by the benzodiazepine lorazepam (0.5 mg/kg, i.p.) for anxiolytic studies, and by the tricyclic anti-depressant, imipramine (10 mg/kg, i.p.), for the antidepressant investigations. Both these standard drugs were administered once, 30 min prior to the tests. WSG induced an anxiolytic effect, comparable to that produced by lorazepam, in the elevated plus-maze, social interaction and feeding latency in an unfamiliar environment, tests. Further, both WSG and lorazepam, reduced rat brain levels of tribulin, an endocoid marker of clinical anxiety, when the levels were increased following administration of the anxiogenic agent, pentylenetetrazole. WSG also exhibited an antidepressant effect, comparable with that induced by imipramine, in the forced swim-induced 'behavioural despair' and 'learned helplessness' tests. The investigations support the use of WS as a mood stabilizer in clinical conditions of anxiety and depression in Ayuweda.
Drug prescription in elderly
(1999) K. Sairam; Ch.V. Rao; R.K. Goel
[No abstract available]
Effect of bioactive tannoid principles of Emblica officinalis on ischemia-reperfusion-induced oxidative stress in rat heart
(Urban und Fischer Verlag Jena, 2002) Salil K. Bhattacharya; D. Bhattacharya; K. Sairam; S. Ghosal
The tannoid principles of the fruits of Emblica officinalis have been reported to exhibit antioxidant activity in vitro and in vivo. In the present study, an emblicanin-A (37%) and -B (33%) enriched fraction of fresh juice of Emblica fruits (EOT) was investigated for antioxidant activity against ischemia-reperfusion (IRI)-induced oxidative stress in rat heart. Vitamin E (VE) was used as the standard antioxidant agent. IRI was induced in isolated rat heart by perfusing it with modified Kreb-Hensleitt's solution for 5 min, followed by a period of ischemia (stoppage of perfusion) for 10 min and then restoring the perfusion (reperfusion) for 15 min. IRI induced a significant decrease in the activities of cardiac superoxide dismutase, catalase and glutathione peroxidase, with a concomitant increase in lipid peroxidation. These IRI-induced effects were prevented by the administration of EOT (50 and 100 mg/kg body wt.) and VE (200 mg/kg body wt.) given orally twice daily for 14 days prior to the sacrifice of the animals and initiation of the perfusion experiments. The study confirms the antioxidant effect or E. officinalis and indicates that the fruits of the plant may have a cardioprotective effect.
Effect of Centella asiatica Linn on physical and chemical factors induced gastric ulceration and secretion in rats
(2001) K. Sairam; Ch.V. Rao; R.K. Goel
Centella asiatica is commonly mentioned as a Rasayana in Ayurveda, an ancient system of Indian medicine for various ailments including abdominal disorders. Rasayanas have been advocated for use in rejuvenation therapy. The present study was conducted to evaluate the possible anti-ulcerogenic activity of fresh juice of C. asiatica (CAJ) against ethanol-, aspirin-, cold-restraint stress- and pyloric ligation induced gastric ulcers in rats. The drug given orally in doses of 200 and 600 mg/kg twice daily for five days, showed significant protection against all the above experimental ulcer models and the results were comparable with those elicited by sucralfate (SF, 250 mg/kg, po, BD × 5 days). CAJ showed little or no effect on offensive acid-pepsin secretion. However, at 600 mg/kg CAJ significantly increased gastric juice mucin secretion and increased the mucosal cell glycoprotiens signifying increase in cellular mucus. It also decreased cell shedding indicating fortification of mucosal barrier. Thus, the ulcer protective effect of CAJ may be due to strengthening of the mucosal defensive factors.
Effect of Convolvulus pluricaulis chois on gastric ulceration and secretion in rats
(2001) K. Sairam; Ch.V. Rao; R.K. Goel
Convolvulus pluricaulis is an indigenous plant commonly mentioned in Ayurveda, an ancient system of Indian medicine, as a rasayana which is mainly advocated for use in rejuvenation therapy. The present study was conducted to evaluate the potential anti-ulcerogenic effect of juice of fresh whole plants of C. pluricaulis (CPJ) against various experimental gastric ulcer models induced by ethanol, aspirin, 2 hr cold restraint stress and 4 hr pyloric ligation in rats. The drug was given orally twice daily for five days in the doses of 375 and 750mg/kg body weight. CPJ showed anti- ulcerogenic effect at both doses in all the experimental gastric ulcer models and was comparable to the reference drug sucralfate (250mg/kg). Gastric juice secretion and mucosal studies were undertaken to find out the possible mechanism of action of antiulcer effect by studying its effects both on offensive and defensive mucosal factors. The antiulcerogenic effect of CPJ was found to be due to augmentation of mucosal defensive factors like mucin secretion, lifespan of mucosal cells and glycoprotiens rather than on the offensive factors like acid-pepsin.
Effect of Ocimum sanctum Linn on peptic ulcers and gastric mucosal offensive and defensive factors
(2000) K. Sairam; C.H.V. Rao; R.K. Goel
Ocimum santum Linn is a commonly mentioned plant in a number of Ayurvedic texts like Caraka samhita, Susrutha and Cakaradutta. Its multifaceted activities has been widely reported. The present study incorporates an extensive study on juiceof fresh leaves of Ocimum santum on gastric ulceration and on various offensive factors like acid and pepsin secretion and defensive mucosal factors like mucin secretion, mucosal cell shedding and glycoproteins. The juice of Ocimum santum was given orally twice daily at 10.00 am and 4.00 p.m. for five days in the doses of 525 and 1050 mg/kg (in terms of dry weight). The result indicated significant antiulcer activity against ethanol, aspirin, 2h cold restraint stress and pyloric ligation induced gastric ulcers. Ocimum santum in the dose of 1050 mg/kg significantly reduced the offensive factors like acid and peptic secretion and Increased the defensive factors like mucin secretion and decreased cell shedding. The antiulcer effect could be due to its effect both on offensive and defensive mucosal factors, which were increased and decreased repectively.
Effect of Piper longum Linn, Zingiber officianalis Linn and Ferula species on gastric ulceration and secretion in rats
(2000) A.K. Agrawal; Ch.V. Rao; K. Sairam; V.K. Joshi; R.K. Goel
Use of Dipaniya Mahakasaya, a group consisting of 10 herbal drugs, has been suggested in Charaka Samhita to improve digestion. Out of these 10 plants, three, viz. P. longum (water decoction), Z. officianalis (water decoction) and Ferula species (colloidal solution) were studied for their antiulcer and mechanism of antiulcer effects in rats. All the drugs in the dose of 50 mg/kg, po, 60 min prior to experiment, showed significant protection against gastric ulcers induced by 2 hr cold restraint stress, aspirin (200 mg/kg, 4 hr) and 4 hr pylorus ligation. The antiulcerogenic effect seemed to be due to the augmentation of mucin secretion and decreased cell shedding rather than offensive acid and pepsin secretion which however, were found to be increased by them.
Effect of standardized extract of Ocimum sanctum Linn. on gastric mucosal offensive and defensive factors
(2005) R.K. Goel; K. Sairam; M. Dorababu; T. Prabha; Ch.V. Rao
The standardized methanolic extract of leaves of O. sanctum (OSE; eugenol content 5%) given in doses of 50-200 mg/kg, orally, twice daily for five days showed dose-dependent ulcer protective effect against cold restraint stress induced gastric ulcers. Optimal effective dose (100 mg/kg) of OSE showed significant ulcer protection against ethanol and pyloric ligation-induced gastric ulcers, but was ineffective against aspirin-induced ulcers. OSE significantly healed ulcers induced by 50% acetic acid after 5 and 10 days treatment. OSE (100mg/kg) significantly inhibited the offensive acid-pepsin secretion and lipid peroxidation and increased the gastric defensive factors like mucin secretion, cellular mucus, and life span of mucosal cells and had antioxidant effect, but did not induce mucosal cell proliferation. The results indicate that the ulcer protective and healing effects of OSE may be due to its effects both on offensive and defensive mucosal factors.
Effect of Withania somnifera glycowithanolides on a rat model of tardive dyskinesia
(Urban und Fischer Verlag Jena, 2002) Salil K. Bhattacharya; D. Bhattacharya; K. Sairam; S. Ghosal
Withania somnifera glycowithanolides (WSG) were investigated for their preventive effect on the animal model of tardive dyskinesia (TD), induced by once daily administration of the neuroleptic, haloperidol (1.5 mg/kg, i.p.), for 28 days. Involuntary orofacial movements (chewing movements, tongue protusion and buccal tremors) were assessed as TD parameters. WSG (100 and 200 mg, p.o.), administered concomitantly with haloperidol for 28 days, inhibited the induction of the neuroleptic TD. Haloperidol-induced TD was also attenuated by the antioxidant, vitamin E (400 and 800 mg/kg, p.o.), but remained unaffected by the GABA-mimetic antiepileptic agent, sodium valproate (200 and 400 mg/kg, p.o.), both agents being administered for 28 days like WSG. The results indicate that the reported antioxidant effect of WSG, rather than its GABA-mimetic action, may be responsible for the prevention of haloperidol-induced TD.
Experimental evaluation of Bocopa monniera on rat gastric ulceration and secretion
(2000) Ch.V. Rao; K. Sairam; R.K. Goel
The anti-ulcerogenic effect of fresh juice from the whole plant of Bocapa monniera Wettst. (BMJ) commonly known as Brahmi in Hindi was examined using gastric ulcer models induced by ethanol, aspirin, 2 h cold restraint stress and 4 h pylorus ligation. Bocaparnonniera juice (BMJ) at doses of 100 and 300 mg/kg and sucralfate at a dose of 250 mg/kg were given orally, twice daily for 5 days. BMJ 100-300 mg/kg produced significant antiulcer activity in all the experimental gastric ulcer models except in case of ethanol-induced ulcers where 100 mg/kg was not found to decrease it significantly. BMJ (100-300 mg/kg) was found to have little or no effect on the offensive acid-pepsin secretion, while cell shedding (μg DNA/mg of protein) and mucin secretion in terms of total carbohydrates: protein ration (TC: P), the two important parameters of defensive factors were significantly decreased and increased respectively indicating enhancement of protective mucosal factors. Both BMJ (300 mg/kg) and SF showed tendency to increase the mucosal glycoproteins in terms of TC : P, though individual carbohydrates and total carbohydrates were either increased or showed a tendency to increase. Thus, ulcer protective effect of BMJ may be due to its effect on mucosal defensive factors like enhanced mucin secretion, mucosal glycoprotein and decreased cell shedding rather than on offensive factors such as acid and pepsin.
Experimental evaluation of Emblica officinalis fruit on gastric ulcers and mucosal offensive and defensive factors in rats
(2001) Ch.V. Rao; K. Sairam; R.K. Goel
Emblica officinalis Gaertn has been mentioned in the Ayurveda as a Maharasayana. The rasayanas are drugs which enhance body resistance and are similar to adaptogens in modern medicine and are advocated for use in rejuvenation therapy. Emblica officinalis is reported to protect the individual against biological, physical and chemical stress manifestations. Stress is considered to be one of the important aetiopathogenic factors for peptic ulceration and hence juice of fresh fruits of Emblica officinalis Gaertn (EOJ) was evaluated for its anti-ulcerogenic effect against various rat gastric ulcers induced by cold restraint stress, pylorus ligation, ethanol and aspirin. A preliminary study done with fresh EOJ (240, 480 or 960 mg.kg-1) administered perorally, twice daily for five days, showed a dose-dependent anti-ulcerogenic effect. A dose of 480 mg.kg-1 was then selected for studying the mechanism of anti-ulcer effect on aggressive acid-pepsin secretion and defensive mucosal factors like mucin secretion, cell shedding and mucosal glycoproteins content in 4th pylorus ligated rats. EOJ showed a decrease in aggressive acid-pepsin concentration and output and increased the defensive mucin secretion and glycoprotein content. It decreased cell shedding indicating a decrease in exfoliation and an increase in life span of cells. Hence anti-ulcerogenic effect of EOJ could be due to its effects both on offensive and defensive mucosal factors.
Gastroduodenal ulcer protective activity of Asparagus racemosus: An experimental, biochemical and histological study
(Elsevier Ireland Ltd, 2003) K. Sairam; S. Priyambada; N.C. Aryya; R.K. Goel
Asparagus racemosus is an Ayurvedic rasayana, which finds mention in ancient Indian texts for treatment of gastric ulcers. The ulcer protective effect of methanolic extract of fresh roots of A. racemosus (ARM), 25-100mg/kg given orally, twice daily for 5 days, was studied on different gastroduodenal ulcer models. ARM 50mg/kg, twice daily, orally (total saponins 0.9%) showed significant protection against acute gastric ulcers induced by cold restraint stress (CRS), pyloric ligation, aspirin plus pyloric ligation, and duodenal ulcers induced by cysteamine. ARM in the above dose also significantly healed chronic gastric ulcers induced by acetic acid after 10 days treatment. However, ARM was ineffective against aspirin- and ethanol-induced gastric ulcers. Further, gastric juice and mucosal studies showed that ARM significantly increased the mucosal defensive factors like mucus secretion, cellular mucus, life span of cells and also possessed significant anti-oxidant effect, but had little or no effect on offensive factors like acid and pepsin. © 2002 Published by Elsevier Science Ireland Ltd.
In vitro evaluation of Bacopa monniera on anti-Helicobacter pylori activity and accumulation of prostaglandins
(Urban und Fischer Verlag Jena, 2003) R.K. Goel; K. Sairam; M. Dora Babu; I.A. Tavares; A. Raman
Bacopa monniera is an Indian tratidional medicine widely used to improve intellectual functions. Earlier, we had reported the prophylactic and curative effects of standardized extract of Bacopa monniera (BME) in various gastric ulcer models. The effect was due to augmentation of the defensive mucosal factors like increase in mucin secretion, life span of mucosal cells and gastric antioxidant effect rather than on the offensive acid-pepsin secretion. The present study includes evaluation of standardized BME (bacoside A content - 35.5 ± 0.9) on other contributing factors towards ulcerogenesis. BME in the dose of 1000 μg/ml showed anti-Helicobacter pylori activity in vitro I and in the dose of 10 μg/ml increased in vitro of prostanoids (PGE and PGI2) in human colonic mucosal incubates. It may be concluded that these factors may contribute to antiulcerogenic activity of BME.
Prophylactic and curative effects of Bacopa monniera in gastric ulcer models
(Urban und Fischer Verlag Jena, 2001) K. Sairam; Ch.V. Rao; M. Dora Babu; R.K. Goel
Bacopa monniera Wettst. (BM, syn. Herpestis monniera L; Scrophulariaceae), is an Ayurvedic drug used as a rasayana. Its fresh juice was earlier reported to have significant antiulcerogenic activity. In continuation, methanolic extract of BM (BME) standardized to bacoside-A content (percentage-38.0±0.9), when given in the dose of 10-50 mg/kg, twice daily for 5 days, showed dose-dependent anti-ulcerogenic on various gastric ulcer models induced by ethanol, aspirin, 2 h cold restraint stress and 4 h pylorus ligation. BME in the dose of 20 mg/kg, given for 10 days, twice daily showed healing effects against 50% acetic acid-induced gastric ulcers. Further work was done to investigate the possible mechanisms of its action by studying its effect on various mucosal offensive acid-pepsin secretion and defensive factors like mucin secretion, mucosal cell sheddings, cell proliferation and antioxidant activity in rats. BME 20 mg/kg showed no effect on acid-pepsin secretion, increased mucin secretion, while it decreased cell shedding with no effect on cell proliferation. BME showed significant antioxidant effect per se and in stressed animals. Thus, the gastric prophylactic and curative effects of BME may be due to its predominant effect on mucosal defensive factors.
Quantitative phytochemical and heavy metal estimation of Mesua ferrea flowers and Argyreia speciosa leaves
(2013) N. Sahu Alakh; S. Hemalatha; K. Sairam
Mesua ferrea Linn. belongs to the family Clusiaceae. The flowers have been cream coloured, ebracteate, pedicellate, pedicel short and axillary. The plant Argyreia speciosa Sweet belongs to the family Convulvulaceae. It is a woody climber. Both the plant parts have been authenticated and identified taxonomically. Defattion of flower and leaves have been done by light petroleum ether 40- 60 and then extracted with ethanol by soxhlet extraction method. Gallic acid has been taken as reference standard for total phenolic estimation. The total phenolic in the ethanolic extract of MF and AS equivalent to gallic acid has been found to be 10.12 and 14.72 mg per gram of dry weight extract respectively. Tannic acid has been taken as reference standard for total tannin estimation. The total tannin in the ethanolic extract of MF and AS equivalent to tannic acid has been found to be 8.75 and 11.25 mg per gram of dry weight extract respectively. Rutin has been used as reference standard for flavonoid and flavonol estimation. The total flavonoid content have been found to be 23 ± 1.37 and 30 ± 0.52 mg / gm plant extract in rutin equivalent respectively. The total flavonol content have been found to be 0.49 ± 0.03 and 3.60 ± 0.25 mg / gm plant extract in rutin equivalent respectively. The saponin content of MF have been 98.75 mg/g and AS have been 7.70 mg/g. Heavy metal estimation has been done by inductively coupled plasma optical emission spectrometer. The heavy metals content of MF have been Cd - 0.001 mg/L, Cr - 0.021 mg/L, Ni - 0.012 mg/L, and Pb - 0.010 mg/L and of AS have been Cd - 0.000 mg/L, Cr - 0.013 mg/L, Ni - 0.021 mg/L, and Pb - 0.011 mg/L.
Role of gastric antioxidant and anti-Helicobactor pylori activities in antiulcerogenic activity of plantain banana (Musa sapientum var. paradisiaca)
(2001) R.K. Goel; K. Sairam; Ch.V. Rao; A. Raman
Studies with plantain banana (Musa sapientum var. paradisiaca) have indicated its ulcer protective and healing activities through its predominant effect on various mucosal defensive factors [Sanyal et.al, Arch Int Pharmacodyn, 149 (1964) 393; 155 (1965) 244]. Oxidative stress and Helicobactor pylori colonization are considered to be important factors in the pathogenesis of gastric ulcers. In the present study methanolic extract of plantain banana pulp (BE) was evaluated for its (i) antiulcer and antioxidant activities in 2 hr cold restraint stress and (ii) anti-H.pylori activity in vitro. The extract (BE, 50 mg/kg, twice daily for 5 days) showed significant antiulcer effect and antioxidant activity in gastric mucosal homogenates, where it reversed the increase in ulcer index, lipid peroxidation and super oxide dismutase values induced by stress. However it did not produce any change in catalase values, which was significantly decreased by stress. Further, in the in vitro study, BE (0.32 - 1000 μg/ml) did not show any anti-H.pylori activity. The results suggest absence of anti-H.pyloric activity of methanolic extract of banana in vitro and its antioxidant activity may be involved in its ulcerprotective activity.