Browsing by Author "Kalicharan Sharma"

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Development of ionic liquid-capped carbon dots derived from Tecoma stans (L.) Juss. ex Kunth: Combatting bacterial pathogens in diabetic foot ulcer pus swabs, targeting both standard and multi-drug resistant strains
(Elsevier B.V., 2023) Nisha Yadav; Deeksha Mudgal; Shivakant Mishra; Hitesh Sehrawat; Niraj Kumar Singh; Kalicharan Sharma; Prabodh Chander Sharma; Jay Singh; Vivek Mishra
Bacterial infection is rapidly growing and is a severe danger to global welfare worldwide. Consequently, there is a growing demand for novel antibacterial medications. As an outcome, the evaluation of antibacterial assets of carbon dots (CDs) including a variety of ionic liquids (ILs) were employed to reside advance notice of the structure tunability of ILs along with abundant surface functional group of designed amine-modified CDs, have recently sparked a lot of interest. In this study for the first time, highly efficient ionic-liquid decorated CDs were synthesized to assess the impact of capped-ionic liquid on the degree of antibacterial activity. Fluorescent amine-modified CDs (CDs-NH2) were obtained from the Tecoma stans (yellow elder flower) extract by a single-step carbonization method in presence of ethylenediamine, and then capped with ionic liquids; 3-(carboxymethyl)-1-methyl-1-H-imidazol-3-ium bromide (ILIM) and 3-(carboxymethyl)-1-methyl-1-H-benzo[d]imidazole-3-ium bromide (ILBIM) by a simple carbodiimide chemistry. In-vitro antibacterial activity of CDs-NH2, ILIM@CDs and ILBIM@CDs were evaluated against common pathogens, Escherichia coli (E. coli) as Gram(-ive) bacteria and Staphylococcus aureus (S. aureus) as Gram(+ive) bacteria as well as against clinical multi-drug resistant strain obtained from pus swab of diabetic foot ulcer, by microdilution method as well as well-diffusion method. The results showed that functional ILs-capped CDs have enhanced antibacterial activity against standard (E. coli, S. aureus) and clinical resistant bacterial strains, with minimal inhibitory concentrations (MICs) values range from 0 to 64 μg/mL for standard E. coli/ S. aureus and 250 μg/mL for drug resistant clinical strains, respectively. Structure activity analysis and molecular docking studies revealed that the antibacterial activity of ILBIM@CDs/ILIM@CDs is particularly better compared to CDs-NH2 and ionic-liquids. © 2023 SAAB