Browsing by Author "Kanchan Yadav"

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Carbon Quantum Dot as Electron Transporting Layer in Organic Light Emitting Diode
(Wiley-Blackwell, 2019) Md. Bayazeed Alam; Kanchan Yadav; Devyani Shukla; Ritu Srivastava; Jayeeta Lahiri; Avanish. S. Parmar
Carbon quantum dots derived from banana leaves by a simple hydrothermal process has been used as an electron transport layer to fabricate Organic Light Emitting Diodes. To the best of our knowledge, this is the first report of Carbon QD utilized as an electron transport layer inOLED. Devices with multilayered structureITO/PEDOT: PSS/PFO/CQD/LiF/Al are fabricated on indium tin oxide (ITO) coated glass substrates. Current-Voltage (I−V) curves reveal that the turn-on voltages are reduced from 8 V to 6 V as compared with pristine PFO device.Results show CQD as an ETL has enhanced the performance and efficiency of the OLEDs. © 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
Effect of carbon quantum dots derived from extracts of UV-B-exposed Eclipta alba on alcohol-induced liver cirrhosis in Golden Hamster
(Springer Nature, 2023) Kshama Rai; Kanchan Yadav; Megha Das; Shilpi Chaudhary; Kaustubh Naik; Priya Singh; Ashutosh Kumar Dubey; Sanjeev Kumar Yadav; Shashi Bhushan Agrawal; Avanish Singh Parmar
The Eclipta alba plant is considered hepatoprotective, owing to its phytoconstituents wedelolactone. In the current study, effect of elevated ultraviolet-B (eUV-B) radiation was investigated on biochemical, phytochemical, and antioxidative enzymatic activities of E. alba (Bhringraj) plant. The UV-B exposure resulted in an increase in oxidative stress, which has caused an imbalance in phytochemical, biochemical constituents, and induced antioxidative enzymatic activities. It was observed that the UV-B exposure promoted wedelolactone yield by 23.64%. Further, the leaf extract of UV-B-exposed plants was used for the synthesis of carbon quantum dots (CQDs) using low cost, one-step hydrothermal technique and its biocompatibility was studied using in vitro MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay on HepG2 liver cell line. It revealed no toxicity in any treatment groups in comparison to the control. Both CQDs and leaf extract were orally administered to the golden hamster suffering from alcohol-induced liver cirrhosis. In the morphometric study, it was clearly observed that a combination of UV-B-exposed leaf extract and synthesized CQDs delivered the best result with maximum recovery of liver tissues. The present study reveals the positive impact of UV-B exposure on the medicinally important plant, increased yield of wedelolactone, and its enhanced hepatoprotective efficacy for the treatment of damaged liver tissues. Graphical Abstract: [Figure not available: see fulltext.]. © 2023, The Author(s), under exclusive licence to European Photochemistry Association, European Society for Photobiology.
Efficient synthesis and in-silico studies of pyrano[3,2-c]pyrones based glycohybrids
(Elsevier B.V., 2024) Kanchan Yadav; Ashish Khanna; Rajdeep Tyagi; Sunil Sharma; Ram Sagar
This work is comprising of designing, efficient synthesis and molecular docking studies of pyrano[3,2-c]pyrones based stereodivergent glycohybrids. The diverse pyrano[3,2-c]pyrones based glycohybrids were efficiently prepared through condensation of 4-hydroxycoumarin derivatives and 4‑hydroxy-6-methyl-2H-pyran-2-ones with various enantiopure 2,3-dideoxy-α,β-unsaturated carbohydrate enals using organo-catalyst, L-proline, in EtOAc at room temperature. 3,4,6-tri-O-acetyl-D-glucal derived acetyl-protected carbohydrate enals yielded highly diastereoselective glycohybrid products. Whereas, poor selectivity was observed with α,β-unsaturated carbohydrate enals obtained from 3,4,6-tri-O-acetyl-D-galactal. The in-silico investigations show the significant binding activity of the selected compounds in the active binding site of HCK protein (PDB: 1QCF) with the better docking score of −7.13 kcal/mol as compared to the co-crystallized ligand PP1 (−6.77 kcal/mol). Additionally, MM/GBSA was calculated using the Prime module to determine the binding energy of the ligands. Selected compounds showed the maximum binding affinity of −74.18 kcal/mol compared to the co-crystallized ligand PP1 (−70.40 kcal/mol). ADMET screening of these compounds predicts the low toxicity and better metabolic profile of the synthesized compounds inside the body. © 2024 Elsevier B.V.
Efficient synthesis of indole-chalcones based glycohybrids and their anticancer activity
(Elsevier Ltd, 2024) Rajdeep Tyagi; Kanchan Yadav; Ashish Khanna; Sunil K. Mishra; Ram Sagar
Indole based glycosides belong to the class of pharmacologically active molecules and found in diverse natural compounds. Herein, we report the synthesis of 1,2,3-triazole bridged chirally enriched diverse indole-chalcones based glycohybrids. Three series of glycohybrids were designed and efficiently synthesized using D-glucose, D-galactose and D-mannose derived 1-azido glycosides. The reactions sequence involved were, the synthesis of indole derived chalcones which were formed via Claisen–Schmidt condensation reaction and subsequently N-propargylation which leads to the production of N-propargylated indole-chalcones. The N-propargylated indole-chalcones get transformed into 1,2,3-triazole bridged indole-chalcone based glycohybrids by reacting with 1-azido sugar glycosides under click-chemistry reaction conditions. Further, the biological activity of synthesized glycohybrids (n = 27) was assessed in-vitro against MDA-MB231, MCF-7, MDA-MB453 cancer, and MCF-10A normal cell lines. The selected compounds showed potent anti-oncogenic properties against MCF-7 and MDA-MB231 breast cancer cell line with IC50 values of 1.05 µM and 11.40 µM respectively, with very good selectivity index (SI > 161). The active compounds show better binding affinity as compared to co-crystallized inhibitor 1-(tert-butyl)-3-(p-tolyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine (PP1) with HCK (PTKs) proteins in molecular docking studies. © 2024 Elsevier Ltd
Ferroelectric liquid crystal nanocomposites: recent development and future perspective
(Taylor and Francis Ltd., 2018) Satya Prakash Yadav; Kanchan Yadav; Jayeeta Lahiri; Avanish Singh Parmar
In recent years, dispersion of nanomaterials in liquid crystal media has attracted a great deal of attention for their applications in various fields and basic understanding. In this regard, nanocomposites of ferroelectric liquid crystals hold a great promise for technological advancement in displays, sensors, development of hybrid materials for optical applications and others. With the emphasis on the properties of ferroelectric liquid crystals, this paper presents a summarizing overview with critical comments on the progress made in last one decade in understanding the influence of nanoparticles on the ferroelectric liquid crystals. The dispersion of nanoparticles in liquid crystal (host material) significantly influences its properties, thereby making the dispersed material more promising for potential applications. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.
Genome mining of Fusarium reveals structural and functional diversity of pectin lyases: a bioinformatics approach
(Springer Science and Business Media Deutschland GmbH, 2022) Kanchan Yadav; Shruti Dwivedi; Supriya Gupta; Amit K. Dubey; Vinay K. Singh; Aiman Tanveer; Sangeeta Yadav; Dinesh Yadav
Pectin lyase (PNL) is an important enzyme of the pectinases group which degrades pectin polymer to 4,5-unsaturated oligogalacturonides by a unique β-elimination mechanism and is used in several industries. The existence of multigene families of pectin lyases has been investigated by mining microbial genomes. In the present study, 52 pectin lyase genes were predicted from sequenced six species of Fusarium, namely F. fujikuroi, F. graminearum, F. proliferatum, F. oxysporum, F. verticillioides and F. virguliforme. These sequences were in silico characterized for several physico-chemical, structural and functional attributes. The translated PNL proteins showed variability with 344–1142 amino acid residues, 35.44–127.41 kDa molecular weight, and pI ranging from 4.63 to 9.28. The aliphatic index ranged from 75.33 to 84.75. Multiple sequence alignment analysis showed several conserved amino acid residues and five distinct groups marked as I, II, III, IV, and V were observed in the phylogenetic tree. The Three-dimensional Structure of five of these PNLs, each representing a distinct group of phylogenetic trees was predicted using I-TASSER Server and validated. The pectin lyase proteins of Fusarium species revealed close similarity with pectin lyase of Aspergillus niger PelA(1IDJ) and PelB(1QCX). Diversity in the structural motifs was observed among Fusarium species with 2 β-sheets, 1 β-hairpin, 7–12 β bulges, 18–25 strands, 6 -11 helices, 1 helix-helix interaction, 32–49 β turns, 2–6 γ turns and 2- 3 disulfide bonds. The unique Pec_lyase domain was uniformly observed among all PNL proteins confirming its identity. The genome-wide mining of Fusarium species was attempted to provide the diversity of PNL genes, which could be explored for diverse applications after performing cloning and expression studies. © 2022, King Abdulaziz City for Science and Technology.
Large area few‐layer hexagonal boron nitride as a raman enhancement material
(MDPI AG, 2021) Nilanjan Basu; Moram Sree Satya Bharathi; Manju Sharma; Kanchan Yadav; Avanish Singh Parmar; Venugopal Rao Soma; Jayeeta Lahiri
Increasingly, two‐dimensional (2D) materials are being investigated for their potential use as surface‐enhanced Raman spectroscopy (SERS) active substrates. Hexagonal Boron Nitride (hBN), a layered 2D material analogous to graphene, is mostly used as a passivation layer/dielectric substrate for nanoelectronics application. We have investigated the SERS activity of few‐layer hBN film synthesized on copper foil using atmospheric pressure chemical vapor deposition. We have drop casted the probe molecules onto the hBN substrate and measured the enhancement effect due to the substrate using a 532 nm excitation laser. We observed an enhancement of ≈103 for malachite green and ≈104 for methylene blue and rhodamine 6G dyes, respectively. The observed enhancement factors are consistent with the theoretically calculated interaction energies of MB > R6G > MG with a single layer of hBN. We also observed that the enhancement is independent of the film thickness and surface morphology. We demonstrate that the hBN films are highly stable, and even for older hBN films prepared 7 months earlier, we were able to achieve similar enhancements when compared to freshly prepared films. Our detailed results and analyses demonstrate the versatility and durability of hBN films for SERS applications. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Reinvestigation of SnCl4 catalyzed efficient synthesis of 2,3-unsaturated glycopyranosides
(Elsevier Ltd, 2023) Yogesh Yadav; Rajdeep Tyagi; Kanchan Yadav; Ghanshyam Tiwari; Ram Sagar
The Ferrier rearrangement is a powerful tool to prepare 2,3-unsaturated glycopyranosides. We have reinvestigated SnCl4 catalyzed Ferrier rearrangements through direct allylic substitution of the hydroxyl group at the C-3 position of glycals, resulting in the formation of stereoselective 2,3-unsaturated glycosides at 0 °C. The catalytic amount of SnCl4 (0.1 equiv.) was successfully used to promote this transformation on 3,4,6-tri-O-acetyl-D-glucal, 3,4,6-tri-O-acetyl-D-galactal and 3,4-di-O-acetyl-D-arabinal using various nucleophiles viz alcohols, azide and thiols to form a variety of 2,3-unsaturated glycopyranosides (pseudoglycals). This straightforward process is notable for its strong anomeric selectivity, excellent yields and shorter reaction time. © 2023 Elsevier Ltd
Synthesis and characterization of novel protein nanodots as drug delivery carriers with an enhanced biological efficacy of melatonin in breast cancer cells
(Royal Society of Chemistry, 2021) Kanchan Yadav; Megha Das; Nurul Hassan; Archana Mishra; Jayeeta Lahiri; Ashutosh Kumar Dubey; Sanjeev Kumar Yadav; Avanish Singh Parmar
Melatonin is a potent antioxidant, chemotherapeutic and chemo preventive agent against breast cancer. However, its short half-life is one of the major limitations in its application as a therapeutic drug. To overcome this issue, the green-emitting protein nanodot (PND) was synthesized by a one-step hydrothermal method for loading melatonin. The synthesized pH-7 and pH-2 PND showed a quantum yield of 22.1% and 14.0%, respectively. The physicochemical characterization of both PNDs showed similar morphological and functional activities. Furthermore, the biological efficacy of melatonin-loaded PND (MPND) was evaluated in a breast cancer cell line (MDA-MB-231) for live-cell imaging and enhanced nano-drug delivery efficacy. Interestingly, the permeability of neutral pH PND in both cell cytoplasm and nucleus nullifies the limitations of real-time live-cell imaging, and ensures nuclear drug delivery efficacy. Neutral pH PND showed better cell viability and cytotoxicity as a fluorescence bioimaging probe compared to acidic PND. The bioavailability and cell cytotoxicity effect of MPND on MDA-MB-231 breast cancer cells were studied through confocal and migration assay. Results showed that MPND causes enhanced bioavailability, better cellular uptake, and inhibition of the migration of breast cancer cells as compared to the drug alone. Besides, the synthesized MPND showed no sign of fluorescence quenching even at a high concentration of melatonin, making it an ideal nanocarrier for bioimaging and drug delivery. © The Royal Society of Chemistry 2021.
Tinospora cordifolia Silver Nanoparticles Attenuated the Lipopolysaccharide-induced Testicular Inflammation in Golden Hamster
(Springer Science and Business Media Deutschland GmbH, 2025) Nitesh Kumar Mishra; Kanchan Yadav; Soumya Ranjan Mohanty; Avanish Singh Parmar; Sanjeev Kumar Yadav; Chandana Haldar
Purpose: Testicular inflammation is one of the primary causes of male infertility. Plant-rich remedies are known to reduce testicular inflammation, but the exact mechanism is not known. Tinospora cordifolia (T. cordifolia), an Indian ayurvedic medicinal plant, possesses excellent immunomodulatory properties. We therefore studied the anti-inflammatory potential(s) of green synthesized silver nanoparticles (AgNPs) from T. cordifolia (TC-AgNPs) on the testes of golden hamster. Methods: Aqueous extract of T. cordifolia was used to synthesize the silver nanoparticles (TC-AgNPs), validated by ultra-high-performance liquid chromatography-high-resolution accurate mass spectrometry (UHPLC-HRMS). Physical characterization was done using UV–Vis spectroscopy, transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared analysis (FTIR) spectroscopy. Biological parameters were measured using enzyme-linked immunosorbent assay (ELISA), western blot analysis, histopathology, photoacoustic, and ultrasound imaging. Results: The UV–Vis surface plasmon resonance (SPR) band at 420 nm confirmed the formation of TC-AgNPs. TEM revealed the semi-spherical morphology of silver nanoparticles with an average diameter of 15 nm. FTIR spectroscopy revealed the presence of multiple functional groups, indicating the bioformulation of silver nanoparticles with T. cordifolia extract. Biological studies with lipopolysaccharide (LPS) challenged male golden hamsters and demonstrated that TC-AgNPs treatment reduced the serum pro-inflammatory cytokine levels (IL-1β, IL-6, and TNF α). The testicular photoacoustic and ultrasound imaging indicated a significant improvement in testicular volume and oxygenation level. Western blot analysis suggests that TC-AgNPs reduced inflammation by downregulating NF-κB protein expression. Furthermore, TC-AgNPs alleviated LPS-induced testicular damages along with an increase in serum testosterone level. Conclusion: The synthesized TC-AgNPs have shown strong anti-inflammatory effects against LPS-induced testicular inflammation in male golden hamsters. Lay Summary: Medicinal plants are increasingly used to treat diseases, with inflammation being a common cause of male infertility. Phytoconstituents are safer and more effective anti-inflammatory agents than synthetic ones. Developing plant-based nanoformulations with high therapeutic efficacy and fewer side effects is essential. T. cordifolia is known for its strong immunomodulatory effects and was used to synthesize TC-AgNPs. Our study found that TC-AgNPs reduced pro-inflammatory cytokines and protected against LPS-induced testicular inflammation in golden hamsters. Further, TC-AgNPs improved testicular volume and oxygen saturation, demonstrating potent anti-inflammatory effects. Future Work/Prospective: Future research should focus on how individual bioactive compounds lower inflammation and explain the molecular mechanisms behind it. © The Author(s), under exclusive licence to The Regenerative Engineering Society 2024.
Tinospora cordifolia Silver Nanoparticles Attenuated the Lipopolysaccharide-induced Testicular Inflammation in Golden Hamster
(Springer Science and Business Media Deutschland GmbH, 2024) Nitesh Kumar Mishra; Kanchan Yadav; Soumya Ranjan Mohanty; Avanish Singh Parmar; Sanjeev Kumar Yadav; Chandana Haldar
Purpose: Testicular inflammation is one of the primary causes of male infertility. Plant-rich remedies are known to reduce testicular inflammation, but the exact mechanism is not known. Tinospora cordifolia (T. cordifolia), an Indian ayurvedic medicinal plant, possesses excellent immunomodulatory properties. We therefore studied the anti-inflammatory potential(s) of green synthesized silver nanoparticles (AgNPs) from T. cordifolia (TC-AgNPs) on the testes of golden hamster. Methods: Aqueous extract of T. cordifolia was used to synthesize the silver nanoparticles (TC-AgNPs), validated by ultra-high-performance liquid chromatography-high-resolution accurate mass spectrometry (UHPLC-HRMS). Physical characterization was done using UV–Vis spectroscopy, transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared analysis (FTIR) spectroscopy. Biological parameters were measured using enzyme-linked immunosorbent assay (ELISA), western blot analysis, histopathology, photoacoustic, and ultrasound imaging. Results: The UV–Vis surface plasmon resonance (SPR) band at 420 nm confirmed the formation of TC-AgNPs. TEM revealed the semi-spherical morphology of silver nanoparticles with an average diameter of 15 nm. FTIR spectroscopy revealed the presence of multiple functional groups, indicating the bioformulation of silver nanoparticles with T. cordifolia extract. Biological studies with lipopolysaccharide (LPS) challenged male golden hamsters and demonstrated that TC-AgNPs treatment reduced the serum pro-inflammatory cytokine levels (IL-1β, IL-6, and TNF α). The testicular photoacoustic and ultrasound imaging indicated a significant improvement in testicular volume and oxygenation level. Western blot analysis suggests that TC-AgNPs reduced inflammation by downregulating NF-κB protein expression. Furthermore, TC-AgNPs alleviated LPS-induced testicular damages along with an increase in serum testosterone level. Conclusion: The synthesized TC-AgNPs have shown strong anti-inflammatory effects against LPS-induced testicular inflammation in male golden hamsters. Lay Summary: Medicinal plants are increasingly used to treat diseases, with inflammation being a common cause of male infertility. Phytoconstituents are safer and more effective anti-inflammatory agents than synthetic ones. Developing plant-based nanoformulations with high therapeutic efficacy and fewer side effects is essential. T. cordifolia is known for its strong immunomodulatory effects and was used to synthesize TC-AgNPs. Our study found that TC-AgNPs reduced pro-inflammatory cytokines and protected against LPS-induced testicular inflammation in golden hamsters. Further, TC-AgNPs improved testicular volume and oxygen saturation, demonstrating potent anti-inflammatory effects. Future Work/Prospective: Future research should focus on how individual bioactive compounds lower inflammation and explain the molecular mechanisms behind it. © The Author(s), under exclusive licence to The Regenerative Engineering Society 2024.