Browsing by Author "Karunamoorthy Vasanth"

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Anti-adipogenic β-sitosterol and lupeol from Moringa oleifera suppress adipocyte differentiation through regulation of cell cycle progression
(John Wiley and Sons Inc, 2022) Karunamoorthy Vasanth; Guha Chowdhury Minakshi; Karthick Velu; Tanu Priya; R. Mohan Kumar; Ilango Kaliappan; Govind Prasad Dubey
Triterpenes and phytosterols enriched herbal formulations are known for glucose regulation and lipid metabolism. In this study, triterpenes and phytosterols from Moringa oleifera stem bark have been tested for their role in adipocyte differentiation. Chromatographic analysis revealed a wide range of phenolics, highlighting the presence of flavonoids (kaempferol, quercetin, and rutin), terpenoids (lupeol), and phytosterol (stigmasterol, β-sitosterol). Lupeol and β-sitosterol reduced cell viability in a dose-dependent manner showcasing increased G1 phase cell accumulation while reducing other cell cycle phases (S and G2/M) and significant lowering of intracellular lipid accumulation. Additionally, lupeol (35.37% at 32 μM) and β-sitosterol (42.97% at 16 μM) inhibited reactive oxygen species generation and increased glucose uptake in adipocytes. Collectively, our results indicate that lupeol and β-sitosterol efficaciously attenuated adipogenesis via a controlled cell cycle progression and enhanced glucose uptake in adipocytes. Practical applications: Active components of Moringa oleifera effectively regulate adipocyte differentation suggest that it can be good medicial supllement for control of obesity. © 2022 Wiley Periodicals LLC.
Anticancer activity of Moringa oleifera mediated silver nanoparticles on human cervical carcinoma cells by apoptosis induction
(2014) Karunamoorthy Vasanth; Kaliappan Ilango; Ramasamy MohanKumar; Aruna Agrawal; Govind Prasad Dubey
Silver nanomaterial plays a crucial role in the growing field of nanotechnology as there is an increasing commercial demand for silver nanoparticles (AgNPs) owing to their wide biological applications. The present investigation aims at developing anti-cancerous colloidal silver using Moringa olifera stem bark extract. Electron and atomic force microscopic images were taken to analyze the surface morphology of the synthesized AgNPs. The effects of synthesized AgNPs were tested against human cervical carcinoma cells (HeLa) and cell morphology was further evaluated using 4,6-diamidino-2-phenylindole (DAPI) staining. The efficiency of green synthesized AgNPs was studied with the help of fluorescence activated cell sorting (FACS) and was shown to induce apoptosis through reactive oxygen species (ROS) generation in HeLa cells. © 2014 Elsevier B.V.
Assessment of serum leptin and resistin levels in association with the metabolic risk factors of pre- and post-menopausal rural women in South India
(2013) Tanu Priya; Minakshi G. Chowdhury; Karunamoorthy Vasanth; Thangavel Mahalingam Vijayakumar; Kaliappan Ilango; Aruna Agrawal; Govind Prasad Dubey
AbstractObjectives Menopausal status is related with weight gain, abnormal lipid and glucose metabolism leading to metabolic syndrome susceptibility. The aim of this study is to determine circulating serum leptin and resistin levels and to correlate these levels in relationship with the metabolic factors in pre- and post-menopausal women. Methods A cross-sectional study has been carried out for 34 subjects who were in post-menopause and 31 subjects who had regular menstruation in south Indian rural women. Anthropometric indices, blood pressure, fasting blood sugar (FBS), fasting lipid profile, fasting leptin and resistin levels were measured. Results In a total of 65 subjects, the mean age of pre-menopausal group was 38.65 ± 6.21 and that of post-menopausal group was 55.32 ± 6.32. Fasting serum leptin level was increased considerably in post-menopausal women when compared to pre-menopausal women (P = 0.018). Resistin has no significant relationship with metabolic factors except Body Mass Index (BMI) in both the groups. Triglycerides and FBS were lower in pre-menopausal group when compared to post-menopausal group (P < 0.001). Leptin was well correlated with BMI in pre-menopausal women (r2 = 0.7120, P < 0.0001) as well as post-menopausal women (r2 = 0.2470, P = 0.0028). Leptin also had significant correlation with FBS in both pre (r2 = 0.1373, P = 0.0402) and post-menopausal women (r2 = 0.2141, P = 0.0401). Systolic blood pressure was positively associated with the leptin levels in post-menopausal women (P < 0.001). Conclusion Leptin was found to have significant association with metabolic factors when compared to resistin in pre- and post-menopausal women and there is no doubt that association of BMI and FBS elevates the level of leptin in both the category. © 2013 Published by Elsevier Ltd on behalf of Diabetes India.
Correlation of serum leptin and resistin levels with the metabolic risk factors of pre- and postmenopausal women in South India
(2013) Tanu Priya; Minakshi G. Chowdhury; Karunamoorthy Vasanth; Thangavel Mahalingam Vijayakumar; Kaliappan Ilango; Aruna Agrawal; Govind Prasad Dubey
Background: Menopausal status is related to weight gain and abnormal lipid and glucose metabolism leading to metabolic syndrome (METS) susceptibility. Purpose: To determine circulating serum leptin and resistin levels and to correlate these levels in relation to the metabolic factors in pre- and postmenopausal women. Methods: A cross-sectional study was carried out on 34 individuals who were postmenopausal and 31 individuals who had regular menstruation among south Indian rural women. Anthropometric indices, blood pressure, fasting blood sugar (FBS), fasting lipid profile, fasting leptin, and fasting resistin levels were measured. Results: In a total of 65 women, the mean age of the premenopausal group was 38.65±6.21 years and that of the postmenopausal group was 55.32±6.32 years. The fasting serum leptin level was increased considerably in postmenopausal women when compared to premenopausal women (p=0.018). Resistin had no significant relationship with metabolic factors except body mass index (BMI) in both of the groups. Triglycerides (TG) and FBS were lower in the premenopausal group when compared to the postmenopausal group (p<0.001). Leptin was well correlated with BMI in premenopausal women (r2=0.7120, p<0.0001) as well as postmenopausal women (r2=0.2470, p=0.0028). Leptin also had a significant correlation with FBS in both pre- (r2=0.1373, p=0.0402) and post-menopausal women (r2=0.2141, p=0.0401). Systolic blood pressure (SBP) was positively associated with leptin levels in postmenopausal women (p<0.001). Conclusion: Leptin was found to have significant association with metabolic factors when compared to resistin in pre- and postmenopausal women and there is no doubt that association of BMI and FBS elevates the level of leptin in both categories. © 2013.
Erratum: Assessment of serum leptin and resistin levels in association with the metabolic risk factors of pre- and post-menopausal rural women in South India (Diabetes and Metabolic Syndrome: Clinical Research and Reviews (2013) 7 (233-237))
(Elsevier Ltd, 2015) Tanu Priya; Minakshi G. Chowdhury; Karunamoorthy Vasanth; Thangavel Mahalingam Vijayakumar; Kaliappan Ilango; Aruna Agrawal; Govind Prasad Dubey
[No abstract available]