Browsing by Author "Komal Agrawal"

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Bio-inspired remediation of wastewater: A contemporary approach for environmental clean-up
(Elsevier B.V., 2022) Divya Singh; Rahul Kumar Goswami; Komal Agrawal; Venkatesh Chaturvedi; Pradeep Verma
Overuse of water has led to the degradation and scarcity of limited water resources, which prompted the modern world to adopt sustainable measures to save water by increasing its reuse and recycling. The use of microbial-based green technology to treat wastewater has appeared to outweigh conventional wastewater treatment (WWT) technologies because this emerging technology overcomes many of the shortcomings of conventional treatment systems. The main objective of this review is to illustrate different types of microorganisms used in various bioreactor configurations for the treatment of polluted water. This review also emphasizes the benefits and applications of using microbes, which play a dual role in treating the wastewater as well as serving as a source of value-added products such as biofuels and biofertilizers. Furthermore, it provides a critical account of the recent integrated green technology approaches such as consortia between microorganisms for the treatment of wastewater. It also critically illustrates the limitations related to green technology, which needs serious attention to facilitate its full-scale development. This review also provides the advantages of green technology and how it preponderates over disadvantages and contributes towards sustainability. © 2022 The Authors
Designing microbial cellulases using genetic engineering approach: A promising strategy towards zero-waste cellulosic biorefinery
(Elsevier Ltd, 2023) Komal Agrawal; Lakshana G. Nair; Venkatesh Chaturvedi; Pradeep Verma
Cellulosic biorefinery is a promising route towards zero-waste environment and sustainable future via the utilization of industrially significant protein i.e., “cellulases”. Despite the advantage, high production costs and low productivity impedes its commercialization. However, to address the same on-site production strategies have been implemented to enhance its production. Additionally, genetic engineering approaches have be used and has allowed to engineer microbes with improved cellulases production abilities. Thus, considering the tremendous potential of cellulases, the current review provides an insight into its structure, production strategies, media optimization along with various factors that impact its production. Further, the review highlights various genetic engineering-based approaches that have been used to increase cellulase production such as heterologous expression, RNA interference (RNAi) technology, CRISPR-Cas9-based technologies etc. Lastly, its applications in various biotechnological sectors, limitations and prospects has been discussed to gain futuristic insight into zero-waste cellulosic biorefinery. © 2023 Elsevier Ltd