Browsing by Author "Krishna P. Singh"

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Ambient UV-B exposure reduces the binding of ofloxacin with bacterial DNA gyrase and induces DNA damage mediated apoptosis
(Elsevier Ltd, 2016) Jyoti Singh; Ashish Dwivedi; Syed Faiz Mujtaba; Krishna P. Singh; Manish Kumar Pal; Deepti Chopra; Shruti Goyal; Ajeet K. Srivastav; Divya Dubey; Shailendra K. Gupta; Chandana Haldar; Ratan Singh Ray
Ofloxacin (OFLX) is a broad spectrum antibiotic, which generates photo-products under sunlight exposure. Previous studies have failed to explain the attenuated anti-bacterial activity of OFLX. The study was extended to explore the unknown molecular mechanism of photogenotoxicity on human skin cell line (HaCaT) under environmental UV-B irradiation. Photochemically OFLX generates ROS and caused 2′-dGuO photodegradation. We have addressed the binding affinity of OFLX and its photo-products against DNA gyrase. Significant free radical generation such as 1O2, O2•- and •OH reduces antioxidants and demonstrated the ROS mediated OFLX phototoxicity. However, the formation of micronuclei and CPDs showed photogenotoxic potential of OFLX. OFLX induced cell cycle arrest in sub-G1 peak. OFLX triggers apoptosis via permeabilization of mitochondrial membrane with the downregulation of anti-apoptotic Bcl-2 and caspase-3 whereas, upregulation of pro-apoptotic Bax and Cyto-C proteins. Our study illustrated that binding affinity of OFLX photo-products with DNA gyrase was mainly responsible for the attenuated antimicrobial activity. It was proved through molecular docking study. Thus, study suggests that sunlight exposure should avoid by drug users especially during peak hours for their safety from photosensitivity. Clinicians may guide patients regarding the safer use of photosensitive drugs during treatment. © 2016 Published by Elsevier Ltd.
Emerging Trends in Plant Pathology
(Springer Singapore, 2020) Krishna P. Singh; Shamarao Jahagirdar; Birinchi Kumar Sarma
This book offers a comprehensive guide to the identification, detection, characterization, classification and management of plant pathogens and other beneficial microbes in agriculture. The science of plant pathology is a dynamic field and, given the growing interest in sustainable agricultural practices, plant disease management has also gained importance. Further, there has been a shift from traditional chemical-based methods to eco-friendly integrated disease management strategies with a greater focus on bio-control and other eco-friendly technologies. This book provides a comprehensive and timely account of latest concepts and advances in the field of plant pathology, including detection and diagnosis, host resistance, disease forecasting and plant biotechnological approaches. Accordingly, it will be of great interest to academics and all stakeholders working in the fields of plant pathology, microbiology, biotechnology, plant breeding, and other life sciences. © Springer Nature Singapore Pte Ltd. 2021.
Metabolite profiling can assist variabilityy analysis in Trichoderma species
(Taylor and Francis Ltd., 2011) Amitabh Singh; Birinchi K. Sarma; Udai P. Singh; Rajesh Singh; Harikesh B. Singh; Krishna P. Singh
Variability in 41 isolates of Trichoderma belonging to 21 species was observed in the phenolic acid profile of their culture filtrates. The phenolic acid profiles were observed to be very stable in the culture filtrate of Trichoderma species. The similarity in phenolic acid profile was recorded and based on it the species were grouped into three distinct groups, viz. highly similar, moderately similar and least similar. Of the 21 species, seven species showed highly similar trend, whereas two and four species showed moderate and least similarity in their phenolic acid profiles, respectively. Looking into the stability of phenolic acid profile in the culture filtrate of the Trichoderma species the present tool may help in diversity analysis in Trichoderma species originating from different geographical areas. © 2011 Taylor & Francis.
Preface
(Springer Singapore, 2020) Krishna P. Singh; Shamarao Jahagirdar; Birinchi Kumar Sarma
[No abstract available]
RETRACTED: Ambient UV-B exposure reduces the binding of ofloxacin with bacterial DNA gyrase and induces DNA damage mediated Apoptosis (International Journal of Biochemistry and Cell Biology (2016) 73 (111–126), (S1357272516300012), (10.10.1016/j.biocel.2016.01.001))
(Elsevier Ltd, 2019) J. Singh; Ashish Dwivedi; Syed Faiz Mujtaba; Krishna P. Singh; Manish Kumar Pal; Deepti Chopra; Shruti Goyal; Ajeet K. Srivastav; D. Dubey; Shailendra K. Gupta; Chandana Haldar; Ratan Singh Ray
This article has been retracted: please see Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). This article has been retracted at the request of the Editor-in-Chief. The study is retracted due to image duplication reasons: The article contains an image that had already appeared in Free Radic Res, 48.3 (2014): 333-346. doi:10.3109/10715762.2013.869324. The images are used in both papers but to conclude something entirely different, and suggested that the images have an entirely different biological meaning and treatment. Duplicating images in this way is ethically not acceptable. © 2019
World Congress on Clinical Nutrition
(2005) Krishna P. Singh; Mandavi Singh; Madhu Goyal
[No abstract available]