Browsing by Author "Kritika Hem"

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Anti-inflammatory and hepatoprotective activities of the roots of Uraria picta
(BRNSS Publication Hub, 2017) Kritika Hem; Narendra Kumar Singh; Manoj Kumar Singh
Introduction: Uraria picta (Jacq.) commonly known as Prishnaparni, is one of the important ingredients of the 10 herb formulation called "Dashmula," used for the treatment of fever and inflammation. Materials and Methods: This study was conducted to investigate the anti-inflammatory and hepatoprotective activities of methanol extract of the roots of U. picta (UPME) at a dose of 100, 200 and 400 mg/kg, p.o. in experimental models of rats. Anti-inflammatory activity of methanol extract was performed by egg albumin-induced and formalin induced rats paw edema. Paracetamol (PCM)-induced liver injury model was used to explore the hepatoprotective activity of UPME. Results: Methanolic roots extract showed significant activity against both models of inflammation. UPME (400, 200, and 100 mg/kg, p.o.) reduced inflammation in egg albumin and formalin treated in dose-dependent manner. Administration of PCM 2000 mg/kg induced liver injury in rats, and therefore, increased the level of enzymes alanine transaminase (ALT), alkaline phosphatase (ALP), and aspartate aminotransferase (AST) in the blood. Administration of UPME 400, 200 and 100 decreased the level of enzymes ALT, ALP and AST significantly which were found comparable with the standard drug silymarin 100 mg/kg. Conclusion: This study demonstrated the ability of U. picta to exert anti-inflammatory and hepatoprotective effects.
Standardization and antioxidant activity of an Ayurvedic formulation "Kushavleha"
(Medknow Publications, 2015) Vineet Sharma; Kritika Hem; Seth Ankit; Santosh Kumar Maurya
Background: Kushavleha, an Ayurvedic polyherbal formulation is an effective medicine for urinary calculi and many more. The present study was carried out to standardize the formulation and established its antioxidant activity in vitro. Methods: The formulation was prepared in the laboratory with authenticated plant drugs. The standardization procedure was conducted on the basis of pharmacognostical and phytochemical parameters as directed by World Health Organization guidelines. Results: Powder study of Kushavleha shows several diagnostic characters such as starch with concentric hilum, pitted vessel, stone cells, fiber with oil globules, pollen grains, lignified fiber epidermis of testa, unicellular trichome and stellate trichome. However some major phytoconstituents like flavonoids, phenolics, tannins, steroids, glycosides, alkaloids and amino acids were found to be positive in preliminary phytoconstituent screening of formulation. Total phenolic, tannin, flavonoid and flavonol content were found to be 144.60 ± 0.41 mg/g in gallic acid equivalent, 123 ± 0.53 mg/g in tannic acid equivalent, 69 ± 0.12 mg/g in rutin equivalent and 0.61 ± 0.27 mg/g in rutin equivalent respectively. Moreover, total solid content, fat content, sugar content, reducing sugar and non reducing sugar were found to be 69 % (w/w), 3.08% (w/w), 68.70% (w/w), 14.21% (w/w) and 54.49% (w/w) respectively. The formulation also exhibited potential antioxidant activity in in vitro DPPH scavenging screening with an IC50 of 63.80 μg/mL. Conclusion: The present result will help in the quality control standardization tool for the manufacturing and processing of Kushavleha.