Browsing by Author "Kumari Anjali"

Now showing 1 - 4 of 4

Assessing the ecological preferences of planktic foraminifera from the south-eastern Arabian Sea
(Elsevier Ltd, 2025) Kumari Anjali; Thejasino Suokhrie; R. Saraswat; Sujata Raikar Kurtarkar; Dinesh Kumar Naik; Dharmendra Pratap Singh; Rupal Dubey
The south-eastern Arabian Sea (SEAS) witnesses the confluence of the Arabian Sea and the Bay of Bengal waters along with an influence from both the summer and winter monsoon. As a result, the distinct hydrographic parameters are expected to modulate the diversity and abundance of planktic foraminifera in this region. Hence, 91 surface sediment samples covering a depth range from 25 m to 3150 m were analysed to establish region-specific planktic foraminiferal ecology. Overall, the water depth, seasonal chlorophyll-a and the mixed layer parameters significantly influence the planktic foraminiferal abundance in the SEAS. A total of 31 species of planktic foraminifera belonging to 17 genera were found. We report Globigerina bulloides as the most dominant species, preferring high productivity regions. Globigerina bulloides and Globigerinoides ruber exhibited opportunistic distribution patterns while region-specific preferences were observed for Globigerinita glutinata, N. dutertrei, Globorotalia cultrata and Trilobatus sacculifer. Globigerinita glutinata show a restricted seasonal occurrence while G. hexagonus is reported as a ubiquitous species. The ecological preferences of the planktic foraminifera inferred from the SEAS will help in reconstructing the past climatic conditions from this region. © 2025 Elsevier Ltd
Association of haplotype and linkage disequilibrium of PARP1 polymorphisms rs1136410, rs1805405 and rs3219088 with gallbladder cancer
(Elsevier B.V., 2023) Kumari Anjali; Tarun Kumar; Amrita Ghosh Kar; Puneet Kumar; Gopeshwar Narayan; Sunita Singh
Background: Previously, we have reported that PARP1 rs1136410 is significantly associated with increased the risk of gallbladder cancer. Aim: We aimed to investigate the association of PARP1 rs1805405 and rs3219088 polymorphisms with risk of GBC and also association of the haplotype and combined effect of PARP1 SNPs (rs1805405 G/A, rs3219088 G/T and rs1136410 A/G). We have also investigated the expression profile of PARP1 and its correlation with polymorphisms, clinical parameters and overall survival. Methods: PARP1 polymorphisms were genotyped by PCR-RFLP and the expression profile of PARP1 at mRNA level was analyzed by semi-quantitative PCR. Overall survival was analyzed using Kaplan-Meier plot and Cox-regression analysis. Results: Haplotype analysis of the PARP1 polymorphisms revealed that AGG, AAG and GGT haplotypes are significantly associated with decreased risk of GBC, while AAT, AGT, GGG and GAG haplotypes are significantly associated with increased risk of GBC. Patients with T1+T2 and treated with chemotherapy having risk genotypes of rs1805405 have decreased overall survival. Upregulation of PARP1 is significantly associated with longer overall survival in patients with GBC with different clinical parameters. SNPs rs1136410 and rs1805405 genotypes are significantly associated with PARP1 expression. Conclusion: Haplotype analysis suggests that PARP1 may have a potential role in gallbladder carcinogenesis. © 2022 Editrice Gastroenterologica Italiana S.r.l.
Association of nonsynonymous SNPs of nucleotide excision repair genes ERCC4 rs1800067 (G/A) and ERCC5 rs17655 (G/C) as predisposing risk factors for gallbladder cancer
(Elsevier B.V., 2022) Kumari Anjali; Tarun Kumar; Puneet Kumar; Gopeshwar Narayan; Sunita Singh
Background: Deregulation of DNA repair mechanisms have been frequently demonstrated in the pathology of cancers including gallbladder cancer. Aim: We aimed to investigate the association of ERCC4 rs1800067 (G/A) and ERCC5 rs17655 (G/C) with the predisposition in gallbladder cancer and its prognosis. We have also investigated the prognostic and diagnostic values of expression profiles of ERCC4 and ERCC5 in GBC. Methods: Polymorphisms of rs1800067 and rs17655 were genotyped by PCR-RFLP. The expression of these genes was analyzed by semi-quantitative PCR. Overall survival was analyzed using Kaplan-Meier plot and cox-regression analysis. Results: Patients with risk group genotypes of rs17655 have shorter overall survival in patients with presence of gallstone, T1+T2 tumor invasion, absence of lymph node involvement and early stages of tumor. Homozygous wild genotype (GG) of rs1800067 and homozygous mutant genotype (CC) of rs17655 together increases two-fold risk of the disease. The variant genotypes (GC/CC) of rs17655 show significantly higher level of ERCC5 expression. Conclusion: Major allele of ERCC4 rs1800067 and minor allele of ERCC5 rs17655 are significantly associated with increased risk of GBC. Upregulation of ERCC4 and ERCC5 is an early event of development of GBC. © 2022
PARP1 rs1136410 (A/G) polymorphism is associated with early age of onset of gallbladder cancer
(Lippincott Williams and Wilkins, 2022) Kumari Anjali; Deepika Singh; Puneet Kumar; Tarun Kumar; Gopeshwar Narayan; Sunita Singh
Objectives Evaluation of the association of PARP1 rs1136410 (A/G) polymorphism with gallbladder cancer susceptibility and its prognosis in the Indian population of eastern Uttar Pradesh and western Bihar. Methods PARP1 rs1136410 was genotyped by PCR-RFLP and its association with the prognosis of gallbladder cancer patients were analyzed using Kaplan-Meier plot and log-rank tests. Results Our results demonstrate that minor allele G is more frequent in gallbladder cancer patients than controls. The frequencies of minor allele G and GG genotype are significantly associated with increased risk of gallbladder cancer. Our data suggest that the minor allele G and homozygous genotype GG are significant predisposing factors for the early age of onset of gallbladder cancer. Similarly, women patients having AG and GG genotypes demonstrate an increased risk of gallbladder cancer. The risk group genotypes (AG + GG) are significantly more frequent in patients with thick gallbladder wall, with jaundice and with the presence of lymph node than in patients with normal gallbladder wall thickness, without jaundice and absence of lymph node involvement. Survival analysis data suggest that patients with risk group genotype (AG + GG) presenting jaundice have shorter overall survival. Conclusion Our study suggests that the minor allele G of PARP1 rs1136410 (A/G) is a predisposing factor for gallbladder carcinogenesis and is significantly associated with early onset of the disease. Interestingly, the minor allele G is significantly more frequent in the patients with jaundice, lymph node metastasis and gallbladder wall thickness. © 2022 Lippincott Williams and Wilkins. All rights reserved.