Browsing by Author "Kumari Swati"

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Antiproliferative Effects of Methanolic Fruit Extract of Solanum xenthocarpum (L.) on Human Breast Cancer Cells
(John Wiley and Sons Inc, 2025) Saumya Maurya; Kumari Swati; S. Suvetha; Mayukh Ghosh; Pavan Kumar Yadav
Solanum xanthocarpum, a perennial herb native to India, contains steroidal glycoalkaloids with notable anticancer properties. This study investigated the antioxidant and antiproliferative effects of methanolic fruit extract of S. xanthocarpum on human breast cancer cells (MDA-MB-231). Phytochemical screening and LC-HRMS analysis confirmed presence of various primary and secondary metabolites. Antioxidant activity was assessed through DPPH, ABTS radical scavenging, reducing power, and phosphomolybdate assays. The extract demonstrated significant antioxidant potential with EC50 values of 60.10±0.88 μg/mL (DPPH) and 392.29±3.93 μg/mL (ABTS). Cytotoxicity against MDA-MB-231 cells was evaluated via morphological analysis, MTT assays, and IC50 determination (24.19±0.56 μg/L). Apoptosis was confirmed using dual staining techniques (AO/EB, Hoechst 33342/PI, DAPI), revealing condensed nuclei, apoptotic bodies, and reduced mitochondrial membrane potential, as indicated by Rhodamine staining. Additionally, increased reactive oxygen species (ROS) levels were observed using H2-DCF-DA staining. The total phenolic and flavonoid contents of the extract were 127.78±3.547 mg GAE/g and 98.06±4.289 mg QE/g, respectively. These findings suggest that the methanolic fruit extract of S. xanthocarpum possesses strong antioxidant and anticancer activities, indicating its potential role in cancer treatment. Further studies are warranted to explore its bioactive compounds for developing novel anticancer therapies. © 2024 Wiley-VHCA AG, Zurich, Switzerland.
Matrix metalloproteases as diagnostic and prognostic biomarkers in cancer
(Elsevier, 2024) Saumya Maurya; Kumari Swati; Mayukh Ghosh; Pavan Kumar Yadav
Matrix metalloproteinases (MMPs) play a crucial role in the degradation and remodeling of the extracellular matrix. The role of MMPs in neoplastic cell invasion, metastasis and cancer progression has been studied extensively. There is a strong relationship between MMP overexpression, development of cancer, and clinical prognosis. Increased levels of one or more members of MMPs have been seen in the majority of malignancies. As a result, it has been anticipated that MMPs may act as both diagnostic and prognostic markers in cancer patients. Certain members of the MMP family have been confirmed as biomarkers of diverse human cancer types. MMPs and prognostic markers are two areas of cancer research that provide critical understanding of disease progression and patient outcomes. Their impact is widespread, from the management of metastatic disease to the early detection of cancer. Therefore, their clinical importance continues to propel advancements in precision medicine and cancer therapy. © 2025 Elsevier Inc. All rights are reserved including those for text and data mining AI training and similar technologies.