Browsing by Author "Lal B. Sharma"

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Appendicectomy under local anaesthesia
(1999) Lal B. Sharma; Mudit Agarwal; Lalit Chaudhary; Vijay K. Shukla
Objective: To analyse our results of appendicectomy under local anaesthesia. Design: Prospective study. Setting: University hospital, India Subjects: 165 patients who presented with appendicitis between January 1996 and December 1997. Intervention and main outcome measures: Appendicectomy after infiltration of local anaesthetic. No patient required general or spinal anaesthesia. Result: Five patients (3%) developed postoperative wound infections. Mean operating time was 20 minutes (range 15-30) and median hospital stay 2 days (range 1-3) Conclusion: Appendicectomy under local anaesthesia is quick, cost-effective and carries little morbidity. It can be safely used for all age groups.
Cytochrome P-450 expression and lipid peroxidation in gallbladder cancer
(2003) Manoj Pandey; Lal B. Sharma; Vijay K. Shukla
Background and Objectives: Carcinoma of the gallbladder is the commonest neoplasm of the biliary tract; however, the etiology of this tumor remains unclear. Methods: A case-control study was carried out comprising 50 patients with gallbladder cancer and 50 patients with cholelithiasis. Cytochrome P-450 estimation was carried out in the gallbladder tissue, and lipid peroxide product melonaldehyde (MDA) was measured in gallbladder bile. Statistical analysis was carried out by Student's t-test, and one-way analysis of variance (ANOVA). The Pearson correlation coefficient was calculated, and linear regression analysis was performed. Results: The mean cytochrome P-450 concentration was significantly higher in patients with carcinoma of the gallbladder than in controls (t = 3.9, P < 0.001; F = 15.2, P = 0.002). Similarly, the mean concentration of melonaldehyde was significantly higher among cancer patients than in patients with gallstones (t = 2.7, P < 0.003; F = 7.37, P = 0.0078). No significant correlation was observed between tissue MDA and cytochrome P-450 levels (Pearson r = 0.1, P = 0.28). Conclusions: The results of the present study show that the rise in concentration of MDA is independent of the rise in concentration of cytochrome P-450; however, this is statistically insignificant. This could be due to biliary stasis leading to a rise in biliary concentration of melonaldeyde that is disproportionate to its production. © 2003 Wiley-Liss, Inc.
Erythrocyte membrane fatty acid profile and saturation index in gallbladder carcinogenesis: A case-control study
(2003) Manoj Pandey; Lal B. Sharma; Shailesh Singh; Vijay K. Shukla
Background: Gallbladder cancer is a common neoplasm of biliary tract, with an unknown etiology. Patients and methods: This study was carried out to evaluate the changes in the membrane fatty acid profile and saturation index in patients with gallbladder cancer. The study sample consisted of 50 newly diagnosed cases of gallbladder cancer and 50 patients undergoing cholecystectomy for cholelithiasis were recruited as controls. The fatty acid estimation was carried out by high performance liquid chromatography (HPLC). Statistical analysis was carried out by student 't' test and one-way ANOVA. Pearson's correlation coefficient was also obtained. Results: A significant lowering of erythrocyte membrane stearic acid (p = 0.000), arachidonic acid (p = 0.001), and saturation index (p = 0.001) was observed in patients with gallbladder cancer compared to controls. A significant inverse relation was observed between stearic and oleic acid (r = -0.269 p = 0.007). Conclusion: Results of the present study suggest an increase in the delta 9 desaturation in cancer patients compared to controls however, a possible role of biliary stasis due to altered gallbladder motility or derangements of signal transduction secondary to altered membrane lipid bilayer cannot be ruled out. © 2003 Pandey et al; licensee BioMed Central Ltd.