Browsing by Author "Laxmi Narayan Gupta"

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Characterization and potential novel applications of zinc-based traditional medicine, Yashad Bhasma
(Elsevier B.V., 2025) Guruprasad C. Nille; Monisha Bhuyan; Laxmi Narayan Gupta; Mohd Ali; Chandra Shekhar Pati Tripathi; Omkar S. Nille; Shardendu K. Mishra; Anuja A. Vibhute; Pranoti Anil Kamble; Himanshu Ranjan; Amaresh Kumar Singh; Arpita P-Tiwari; Anand Kumar Chaudhary
Background: Yashad Bhasma (YB), the incinerated metal ash of zinc, has been used for centuries in Ayurveda to address a variety of conditions, including eye diseases, diabetes mellitus, anemia, respiratory illnesses, etc. Objective: This research aimed to synthesize and characterize YB and to evaluate its potential antimicrobial, antioxidant, and anti-angiogenic activities. Materials and methods: In this study, YB is synthesized by optimizing the traditional method. Morphological and physicochemical characterization are performed using XRD, XPS, SEM, TEM, EDAX, DLS, TGA-DSC, and FTIR. The antimicrobial activity of YB is assessed using the well diffusion technique against the gram-positive bacterium Staphylococcus aureus (S. aureus) and the gram-negative bacterium Escherichia coli (E. coli). The antioxidant potential is evaluated using the 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) radical scavenging assay and Nitric oxide (NO) radical scavenging assay. A chick chorioallantoic membrane (CAM) assay is performed on fertilized chick eggs to study the anti-angiogenesis potential of YB. Results: The XRD patterns of YB showed the presence of cubic and hexagonal phases of ZnS having average crystallite size of 32.66 nm. XPS data supports the formation of ZnS phase of YB. SEM and TEM data confirmed the size of YB NPs in a range of 250–350 nm. The EDAX analysis confirmed the presence of Zn (37.2 %) and S (21.18 %). The mean particle diameter was 361 nm in DLS. TGA-DSC findings verified that the synthesized material is stable up to 435.80 °C. The FTIR confirms the presence of organic moieties in YB along with ZnS phase. YB effectively inhibited the growth of S. aureus and E. coli. The ability of YB to scavenge DPPH and NO radicals is found to be concentration dependent (50–250 μg/mL). The study also demonstrated that YB has notable antioxidant activity. The disappearance of blood vessels beneath the sample-loaded disk after 7 days indicated the effective anti-angiogenic properties of YB. Conclusion: Altogether, YB exhibited significant antimicrobial, noteworthy antioxidant, and anti-angiogenic activities, indicating its potential as a promising therapeutic agent. © 2025 The Authors
Safe and effective management of psoriasis through Ayurveda: A case report
(Elsevier B.V., 2025) Guruprasad C. Nille; Monisha Bhuyan; Laxmi Narayan Gupta; Anand Kumar Chaudhary
Ayurveda, an ancient system, offers in-depth insights into various skin disorders. It provides detailed understanding of causative factors and pathomechanisms, along with time-tested traditional treatments based on its principles. We report a 16-year chronic case of psoriasis (36-year-old male patient) with plaques and peeling erythrodermic skin rashes over the back and front of the trunk area and both legs. The scalp area and nail beds were also affected. The early involvement of joints was also noted. A two-year multimodal Ayurveda treatment has shown notable efficacy in managing chronic plaque and erythrodermic psoriasis, with no reported adverse events or side effects. The progressive events were documented in the form of photographs with the proper consent of the patient. The Ayurveda medicines, strict dietary regimen, and regular follow-ups together resulted in promising outcomes, revalidating the potential of Ayurveda in controlling psoriasis and its complications. © 2024 The Authors
Standard manufacturing procedure of Teekshna lauha bhasma
(Elsevier B.V., 2016) Thakur Rakesh Singh; Laxmi Narayan Gupta; Neeraj Kumar
Background Lauha bhasma is one of the herbo-metallic preparations used in Ayurveda, a traditional Indian system of medicine for treating various ailments such as anemia, diarrhea, hyperlipidemia and diabetes. Objective To establish standard manufacturing procedure of Teekshna lauha bhasma and analyze its physico-chemical properties. Materials and methods The preparation of T. lauha bhasma (calx of iron [Fe] turning) involves samanya shodhana, vishesha shodhana followed by bhanupaka, sthalipaka and putapaka with Triphala kwatha as a medium under temperature of 650 °C in electric muffle furnace (EMF) and maintained for 1 h. T. lauha bhasma were subjected to different physico-chemical characterization using X-ray fluorescence spectrophotometer and scanning electron microscopy. Results and discussion The results suggest that these steps are necessary to obtain a good quality of bhasma and also make it acceptable for trituration during Bhasmikarana process. It is found that T. lauha bhasma was prepared properly in 20 puta at a temperature of 650 °C. The particle size of 20 puta T. lauha bhasma is 100–500 nm in range. Conclusion Pharmaceutical procedures given in Ayurvedic texts are necessary to prepare pakwa jambu phala varna T. lauha bhasma that complies with all the classical bhasma pariksha and modern analytical parameters in 20 puta at a temperature of 650 °C maintained for 1 h in EMF. © 2016 Transdisciplinary University, Bangalore and World Ayurveda Foundation