Browsing by Author "Laxmi Upadhyaya"

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Efficient water soluble nanostructured ZnO grafted O-carboxymethyl chitosan/curcumin-nanocomposite for cancer therapy
(Elsevier Ltd, 2015) Laxmi Upadhyaya; Jay Singh; Vishnu Agarwal; A.C. Pandey; Shiv P. Verma; Parimal Das; R.P. Tewari
The present work deals with the synthesis of efficient water soluble O-carboxymethyl chitosan (O-CMCS) based nanocomposites (NCs) with nanostructured zinc oxide (n-ZnO) by ex-situ grafting method for the delivery of anticancer drug curcumin (Cr). The phase identification, morphology and thermal stability of prepared Cr/O-CMCS/n-ZnO NCs have been investigated by FT-IR, XRD, SEM, TEM and TGA/DTA techniques. The drug entrapment efficiency have found to be 74% and in vitro drug release study performed at 37 °C at pH 4.5 and 7.4 indicated slow and controlled release in the initial phase and sustained in later phase. The MTT assay showed higher and preferential toxicity of the Cr/O-CMCS/n-ZnO NCs against cancer cells (MA104). The cellular uptake study by FACS revealed concentration dependent uptake of the NCs. The utilization of this Cr/O-CMCS/n-ZnO nanoformulation offers an efficient strategy and a novel promising soluble nanomatrix for anticancer therapy and other biomedical applications. © 2015 Elsevier Ltd. All rights reserved.
In situ grafted nanostructured ZnO/carboxymethyl cellulose nanocomposites for efficient delivery of curcumin to cancer
(Kluwer Academic Publishers, 2014) Laxmi Upadhyaya; Jay Singh; Vishnu Agarwal; A.C. Pandey; Shiv P. Verma; Parimal Das; R.P. Tewari
In this present manuscript, zinc oxide (ZnO) nanoparticles embedded carboxymethyl cellulose (CMC) bionanocomposite were prepared by in situ grafting and the hydrophobic anticancer drug curcumin (Cur) was loaded into it. Structural, morphological, and physiochemical behavior of prepared curcumin-loaded CMC/ZnO nanocomposites (NCs) were characterized by FTIR, XRD, SEM, TEM, TGA, and DTA. The drug entrapment efficiency was evaluated and the in vitro efficacy as anticancer drug delivery vehicle was analyzed. The potential toxicity of curcumin-loaded ZnO/CMC NCs (Cur/ZnO/CMC NCs) was studied by using L929 and MA104 cell lines via MTT assay. The cellular uptake study of Cur/ZnO/CMC NCs by normal (L929) and cancer (MA104) cells carried out by using ethanol extraction and by FACS analysis has been reported. The results of this investigation demonstrate that the nanomatrix synthesized can effectively deliver the anticancer drug curcumin, and hence appears to be a promising nanoformulation for anticancer therapy and other biomedical applications. © 2014, Springer Science+Business Media Dordrecht.