Browsing by Author "M. Owais"

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Clinical usefulness of a modified imunoelectrophoresis method for detection of myeloma immunoglobulins
(Nova Science Publishers, Inc., 2011) Nadeem A. Ansari; M. Owais; Usha
Immunoelectrophoresis is a clinically prevalent and sensitive technique used for isotyping of multiple myeloma. Immunoglobulin heavy and light chain types are known as potential biomarkers for the disease. In the present study, we used a modified immunoelectrophoresis method to detect the immunoglobulins of myeloma patients. Immunoglobulins of myeloma patients showed sharp precipitin bands while that of normal healthy subjects showed no characteristic bands of myeloma. The method proved to be rapid and cost effectivewith no loss of sensitivity for detection of precipitin bands corresponding to immunoglobulin heavy and light chains of myeloma patients. © 2011 Nova Science Publishers, Inc. All rights reserved.
Immunoglobulin heavy and light chain isotypes in multiple myeloma patients
(Asian Pacific Organization for Cancer Prevention, 2007) Nadeem A Ansari; M. Owais; Usha
The frequency of expression of immunoglobulin (Ig) light and heavy chain isotypes was analyzed in myeloma proteins (M-proteins) from sera of 40 Indian patients with clinically established multiple myeloma. Patients samples were screened by a combination of electrophoresis, immunoelectrophoresis (IEP) and ELISA techniques in this study. We found that majority of the myeloma proteins (58%) were of the IgG isotype followed by IgA (24%) and biclonal gammopathy associated with IgG and IgA (5%). Both kappa and lambda light chains were associated with the heavy chain isotypes. We recommend the triangular combination for detection of M-proteins and biclonal gammopathy of cancerous plasma cells as biomarkers for diagnosis of myeloma.