Browsing by Author "M.K. Gupta"

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Cytokine gene variants and treatment outcome of cisplatin-based concomitant chemoradiotherapy in cervical cancer
(Taylor and Francis Ltd., 2020) A.S. Kushwah; M.K. Gupta; R. Singh; M. Banerjee
Background: Cervical cancer is the second most common cancer among women after breast cancer. Its standard treatment is cisplatin-based concomitant chemoradiotherapy. Chronic inflammation in uterine cervix triggers both pro- and anti-inflammatory pathways. The unpredictability in toxicity and efficacy of treatment is a major challenge. We hypothesized a link between IL-1, IL-6 and TNF gene variants and treatment response. Material & Methods: We genotyped 246 cervical cancer cases and 246 controls by PCR, PCR-RFLP and ARMS-PCR. Treatment and response were evaluated by RECIST criteria. Chemotherapy and radiation doses were same for all patients, whilst 48 were followed-up for 36 months after treatment. Results: SNPs in IL-1RN, IL-1β, IL-6 and TNFα were linked with cervical cancer. Cases with certain allele combinations in IL-1RN, IL-1β, IL-6(-597A/G) and TNF-α showed odds ratios (95% CI) of up to 17.54 (2.7–24.08) for the presence of cervical cancer. Variant IL-1β (-511T/C) was linked to vital status but none were linked to overall survival. Conclusion: Certain cytokine gene variants may help detect susceptibility to cervical cancer and predict response to chemoradiotherapy. © 2020, © 2020 British Journal of Biomedical Science.
Genotypic analysis of XRCC4 and susceptibility to cervical cancer
(Taylor and Francis Ltd., 2020) M.K. Gupta; A.S. Kushwah; R. Singh; M. Banerjee
Background: XRCC4 encodes a DNA repair protein which maintains genome stability by repairing double-strand breaks by the error-prone method. Defects in the protein-encoding gene lead to impairment of DNA repair process and accumulation of DNA damage, a hallmark of cancer development. We hypothesised that variants in XRCC4 are linked to cervical cancer. Material and methods: Genotyping of XRCC4 variants viz. intron3 DIP (rs28360071), intron7 DIP (rs28360017), G-1394T(rs6869366) and G-652T (rs2075685) was carried out in 246 women with cervical cancer cases and 246 control women. Results: There were several links to cervical cancer: intron3 DIP (rs28360071) II genotype (p = 0.002) and I allele (odds ratio is 0.54–0.89) (p = 0.004), intron7 DIP (rs28360017) II genotype (p = 0.003) and I allele (odds ratio 0.68 [0.53–0.88]) (p = 0.004), and G-652T (rs2075685) genotype (p = 0.044) and the T allele (odds ratio 1.35 [1.03–1.77]) (p = 0.032). In combining data into haploviews, the DDGG allele combination had an odds ratio of 0.12 (0.04–0.39) (p= 0.029) and the IIGT combination an odds ratio of 3.08 (1.25–7.55) (p = 0.01) for cervical cancer. Conclusion: Our results suggested that homozygous ‘I’ and ‘T’ genotypes in certain XRCC4 sequences may be associated with the development of cervical cancer and so may be a useful biomarker to predict cervical cancer susceptibility. © 2019, © 2019 British Journal of Biomedical Science.
Necessity to identify the causative agent for appropriate treatment in fungal corneal ulcer: an in vitro study
(Elsevier Masson SAS, 2018) M.K. Gupta; A. Chandra; P. Prakash; R. Tilak
Objective: Fungal corneal ulcers are a major cause of preventable blindness. Different antifungal agents as natamycin, nystatin, fluconazole, itraconazole, voriconazole are used to treat these ulcers. Among these, natamycin is most widely used as a treatment modality. In natamycin non-responding cases, other drugs especially voriconazole is used. This study was done to assess the use of antifungal drugs in the treatment of fungal corneal ulcer by determining the minimum inhibitory concentration against common fungal pathogens. Material and methods: Minimum inhibitory concentration of fluconazole, itraconazole, voriconazole, nystatin and natamycin was assessed against the 61 isolated corneal fungal pathogens as per CLSI guidelines. Results: MIC value of different antifungal agents varies as per fungal strains. Voriconazole showed the lowest MIC against the isolated fungi, in comparison to fluconazole and itraconazole. In comparison to other fungi, higher natamycin MIC was observed against Aspergillus species. Itraconazole is poorly effective against Fusarium sp. Conclusion: Identification of causative fungi is necessary before antifungal treatment. Lowest voriconazole MIC promotes its use as 1st line drug. Comparative higher natamycin MIC, especially against Aspergillus species, warns clinician to have MIC in each case of a non-responding fungal corneal ulcer. © 2017
Scanning tunneling microscopic studies of growth spirals in bulk single crystals of superconducting YBa2Cu3O7 and flux pinning
(1992) A.V. Narlikar; P.K. Dutta; S.B. Samanta; O.N. Srivastava; P. Ramasamy; S.C. Sabarwal; M.K. Gupta; B.D. Padalia
Growth spirals have been observed in the basal plane of bulk single crystals of Y-123 high Tc cuprate, using scanning tunneling microscope with the unit cell resolution. The density of screw dislocations present is of the order of 107-108/cm2, close to the recently reported values for the sputtered epitaxial thin films of Y-123. The growth steps or terraces which are generally shown to possess a step height equal to the c parameter of the unit cell, have been further resolved into substeps corresponding to each of the triple perovskite block. Further, in the limit of ultimate resolution we have been able to demonstrate the substep heights corresponding to the individual atomic layers of the unit cell and thus provide a persuasive evidence that single crystals of Y-123 grow via layer-by-layer growth on the atomic scale within the purview of the spiral growth mechanism. Growth features are briefly discussed and it is argued that the observed screw dislocation density is still too low and the orientation is not favorable for realizing optimum flux pinning in these HTSC systems.
STM studies of growth spirals in bulk single crystals of superconducting Y-123 and Bi-2122
(1992) A.V. Narlikar; P.K. Dutta; S.B. Samanta; O.N. Srivastava; P. Ramasamy; S.C. Sabarwal; M.K. Gupta; B.D. Padalia
The STM observations of growth spirals in bulk single crystals of Y-123 and Bi-2122 high Tc cuprates are reported for the first time. The density of screw dislocations present is of the same order as recently reported for sputtered epitaxial thin films of Y-123. Growth features are briefly discussed and it is argued that the observed dislocation density is still too low for realizing optimum flux pinning in these HTSC systems. © 1992.
Synthesis and antibacterial activity of semicarbazones and thiosemicarbazones
(Chemical Publishing Co., 2007) M.K. Gupta; A.K. Sachan; S.N. Pandeya; V.S. Gangwar
Aryl semicarbazones and thiosemicarbazones have been synthesized by the condensation of aromatic carbonyl compounds with semicarbazide and thiosemicarbazide compounds respectively. The compounds have been characterized on the basis of analytical and spectral data. They have been screened for antibacterial activity by agar double dilution method. Some of them have significant activity against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa.