Browsing by Author "Mahalingam R. Vijayakumar"

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Transferrin liposomes of docetaxel for brain-targeted cancer applications: formulation and brain theranostics
(Taylor and Francis Ltd, 2016) Sonali; Rahul Pratap Singh; Nitesh Singh; Gunjan Sharma; Mahalingam R. Vijayakumar; Biplob Koch; Sanjay Singh; Usha Singh; Debabrata Dash; Bajarangprasad L. Pandey; Madaswamy S. Muthu
Diagnosis and therapy of brain cancer was often limited due to low permeability of delivery materials across the blood–brain barrier (BBB) and their poor penetration into the brain tissue. This study explored the possibility of utilizing theranostic d-alpha-tocopheryl polyethylene glycol 1000 succinate mono-ester (TPGS) liposomes as nanocarriers for minimally invasive brain-targeted imaging and therapy (brain theranostics). The aim of this work was to formulate transferrin conjugated TPGS coated theranostic liposomes, which contain both docetaxel and quantum dots (QDs) for imaging and therapy of brain cancer. The theranostic liposomes with and without transferrin decoration were prepared and characterized for their particle size, polydispersity, morphology, drug encapsulation efficiency, in-vitro release study and brain theranostics. The particle sizes of the non-targeted and targeted theranostic liposomes were found below 200 nm. Nearly, 71% of drug encapsulation efficiency was achieved with liposomes. The drug release from transferrin conjugated theranostic liposomes was sustained for more than 72 h with 70% of drug release. The in-vivo results indicated that transferrin receptor-targeted theranostic liposomes could be a promising carrier for brain theranostics due to nano-sized delivery and its permeability which provided an improved and prolonged brain targeting of docetaxel and QDs in comparison to the non-targeted preparations. © 2016 Informa UK Limited, trading as Taylor & Francis Group.
Transferrin receptor-targeted vitamin E TPGS micelles for brain cancer therapy: preparation, characterization and brain distribution in rats
(Taylor and Francis Ltd, 2016) Sonali; Poornima Agrawal; Rahul Pratap Singh; Chellappa V. Rajesh; Sanjay Singh; Mahalingam R. Vijayakumar; Bajrangprasad L. Pandey; Madaswamy Sona Muthu
The effective treatment of brain cancer is hindered by the poor transport across the blood–brain barrier (BBB) and the low penetration across the blood–tumor barrier (BTB). The objective of this work was to formulate transferrin-conjugated docetaxel (DTX)-loaded d-alpha-tocopheryl polyethylene glycol 1000 succinate (vitamin E TPGS or TPGS) micelles for targeted brain cancer therapy. The micelles with and without transferrin conjugation were prepared by the solvent casting method and characterized for their particle size, polydispersity, drug encapsulation efficiency, drug loading, in vitro release study and brain distribution study. Particle sizes of prepared micelles were determined at 25 °C by dynamic light scattering technique. The external surface morphology was determined by transmission electron microscopy analysis and atomic force microscopy. The encapsulation efficiency was determined by spectrophotometery. In vitro release studies of micelles and control formulations were carried out by dialysis bag diffusion method. The particle sizes of the non-targeted and targeted micelles were <20 nm. About 85% of drug encapsulation efficiency was achieved with micelles. The drug release from transferrin-conjugated micelles was sustained for >24 h with 50% of drug release. The in vivo results indicated that transferrin-targeted TPGS micelles could be a promising carrier for brain targeting due to nano-sized drug delivery, solubility enhancement and permeability which provided an improved and prolonged brain targeting of DTX in comparison to the non-targeted micelles and marketed formulation. © 2015 Informa UK Limited, trading as Taylor & Francis Group.