Browsing by Author "Manish Kumar Singh"

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A coumarin based fluorescent probe enabling nanomolar detection of Zn2+ and Cu2+
(Elsevier B.V., 2022) Virendra Kumar; Uzra Diwan; Nidhi Tyagi; Rakesh K. Mishra; Manish Kumar Singh; K.K. Upadhyay
A rationally designed fluorescent probe constructed on a coumarin platform (R-1) distinguished sequentially & distinctly two vital ions viz. Zn2+ and Cu2+ through different mechanistic approaches at their nanomolar concentrations. The native dark state (off-state) of R-1 was illuminated (on-state) upon its interaction with Zn2+ and further reverted back to its natural dark state in the presence of Cu2+ in aqueous medium. The structural details of R-1 and its ensemble with Zn2+ were unambiguously elucidated through their single-crystal XRD studies. The single crystals of R-1-Zn2+ with chloride and acetate counter anions displayed a fascinating 1-D co-ordination polymeric supramolecular structures. The strategic incorporation of pyridine –N in R-1 introduced a yet another binding site and consequently modulated its optical characteristics, while the analogous derivative R-3, inspite of having electron withdrawing –NO2 group, (instead of pyridine –N as in R-1 and R-2) was unable to produce any significant sensing response under similar conditions. The corresponding optical responses with Zn2+/Cu2+ were equated with INHIBIT logic- gate function. Moreover, the R-1 was deployed for imaging the above ionic analytes in rat glioblastoma cell lines (c6) through confocal laser scanning microscopy. © 2021 Elsevier B.V.
A pyrene-benzthiazolium conjugate portraying aggregation induced emission, a ratiometric detection and live cell visualization of HSO3-
(Elsevier B.V., 2016) Uzra Diwan; Virendra Kumar; Rakesh K. Mishra; Nishant Kumar Rana; Biplob Koch; Manish Kumar Singh; K.K. Upadhyay
The present study deals with the photophysical property of a pyrene-benzthiazolium conjugate R1, as a strong intramolecular charge transfer (ICT) probe exhibiting long wavelength emission in the red region. Unlike traditional planar polyaromatic hydrocarbons whose aggregation generally quenches the light emission, the pyrene based R1 was found to display aggregation-induced emission (AIE) property along with simultaneous increase in its quantum yield upon increasing the water content of the medium. The R1 exhibits high specificity towards HSO3-/SO32- by interrupting its own ICT producing there upon a large ratiometric blue shift of ~220 nm in its emission spectrum. The lowest detection limit for the above measurement was found to be 8.90 × 10-8 M. The fluorescent detection of HSO3- was also demonstrated excellently by test paper strip and silica coated TLC plate incorporating R1. The live cell imaging of HSO3 through R1 in HeLa cells was studied using fluorescence microscopic studies. The particle size and morphological features of R1 and R1-HSO3- aggregates in aqueous solution were characterized by DLS along with SEM analysis. © 2016 Elsevier B.V.
A radical approach for fluorescent turn 'on' detection, differentiation and bioimaging of methanol
(Royal Society of Chemistry, 2015) Virendra Kumar; Ajit Kumar; Uzra Diwan; Manish Kumar Singh; K.K. Upadhyay
A simple Schiff base (RC) has been explored as a smart example of fluorescent material for the selective detection, differentiation and bioimaging of methanol. The nucleophilic attack of methanol on the cyclic control unit of RC leads to its opening and formation of a highly fluorescent moiety, RO. The RC displays a good sensitivity for MeOH with a detection limit of 0.042 wt% in water. © The Royal Society of Chemistry 2015.
Analysis of potential neuropharmacological activity and attenuating effect in chronic constriction induced neuropathic pain using Calotropis procera (Aiton) Dryand flower ethanol extract
(Elsevier B.V., 2025) Ashutosh Kumar; Brijesh Pawan Kumar; Raj Kumar; Vinod N. Tiwari; Pratistha K. Singh; Ajay Kumar; Manish Kumar Singh; Chandra Shekhar Azad; Ankit Uniyal
Background: Calotropis procera, also known as "毒竹 du zhu" in Chainese, is used in several remedies to treat a variety of ailments, including inflammatory diseases, skin concerns, pain disorders, and respiratory issues. It has been observed that the various parts of the plant have been traditionally used for as anti-inflammatory and neuroprotective actions. The flower of this herb has not been investigated for these pharmacological properties. Purpose: The aim of this research is to investigate the neuropharmacological profile and ameliorative potential of ethanolic extract of C. procera flower (EECP) in chronic constriction injury (CCI) induced neuropathic pain in rats. Methods: GCMS analysis was performed to identify the active phytocmpounds of the plant. Neuropharmacological profile has been investigated by maximal electroshock seizure and pentylenetetrazole for antiepileptic, elevated maze plus and open field test for anxiety, tail suspension and forced swim test for depressant activity, and acetylcholinesterase and Morris water maze test for cognition. Anti-neuropathic pain was assessed via heat hyperalgesia and mechanical allodynia tests in rats after inducing CCI. Pro-inflammatory mediators (TNF-α, IL-1β, and IL-6) were determined by ELISA kits. SOD and nitrile level were measured for antioxidant activity. Sciatic nerve's histopathological changes for nerve deformity were evaluated by H &E staining. Results: GCMS analysis revealed the presence of phytocompounds Lupeool, acetate, n-hexadecanoic acid, γ-sitosterol, hexadecanoic acid methyl ester, Octadecenoic acid (Z)-, methyl ester, β-Amyrin, phytol and other compounds. In neuropharmacological profile, EECP had a significant anticonvulsant effect, a decrease in locomotor activity, indicating a sedative effect but showed no anxiolytic effect. The immobility time decreased significantly in both the forced swim test and tail suspension test. The activity of acetylcholinesterase in the brain was decreased and Morris water test results revealed a shorter escape latency and greater time spent in the target quadrant. In anti-neuropathic pain assessment, the EECP reduced CCI-induced hyperalgesia and mechanical allodynia. TNF-α, IL-1β, and IL-6 levels were reduced while SOD levels increased and nitrite levels decreased in the sciatic nerve. Histological analysis revealed sciatic nerve deformity was reduced. Conclusion: It is concluded that extract showed a potent antiepileptic, antidepressant, cognition enhancer and protective against nerve deformity and neuropathic pain. Phytocompounds identified via GCMS having neuroprotective, antioxidant, and anti-inflammatory properties, which may be correlated with the neuropharmacological and analgesic activities of the extract. © 2025
Bioactive metabolites identification of the foxnut and broken millet-based nutritional bar using HR-MS
(Elsevier B.V., 2024) Vishal Kumar; Arvind Kumar; Manish Kumar Singh; Priya Dhyani; Himanshu Mishra; Dinesh Chandra Rai
The by-products of the grain processing industry are a vital resource for the valorization methods in the food industry. In comparison to the whole grain, the broken kernels and seeds own similar nutrient and bioactive compounds having multifaceted health properties. This study aims to develop a nutritional bar by utilizing the by-products from barnyard millet and foxnut with added sweeteners. Furthermore, high-resolution mass spectrometry (HR-MS) metabolomics was carried out in positive and negative both ion modes to identify the major bioactive compounds formed in the matrix of the best-optimized valorized bar. The formulation of the bar having 15 % foxnut flour and the barnyard flour each, was elucidated highest rheological and sensory scores. A sum of 29 bioactive metabolites has been observed in the obtained metabolome. Major metabolites were palmitoyl serinol, glycitein, persin, bufagargarizin, apigenin, carvone, etc. covering a wide area in the mass spectrum. The therapeutic value of these compounds is heart health promotion, anti-inflammatory, anti-carcinogenic, anti-diabetic, anti-microbial, etc. This work highlights the bioactivity of the valorized nutritional bar employing robust and accurate tool of mass spectrometry. The developed snack is a functional food for the consumers. © 2024 The Author(s)
Contagion effect of COVID-19 outbreak: Another recipe for disaster on Indian economy
(John Wiley and Sons Ltd, 2020) Manish Kumar Singh; Yadawananda Neog
The global outbreak of COVID-19 and subsequent lockdowns raises serious economic concern and havoc worldwide. This article tries to provide an economic perspective of COVID-19 in India using some statistical figure of economic indicators. Analysis is based on the data collected for macro-economy, travel & tourism, transportation, stock market, human capital and trade. Finding revel that India could experience a health debacle at present and excruciating economic contraction in the near future if the government is unable to execute a proper policy framework. Based on the discussion, a few policy suggestions have been presented to counter both health and economic crisis. © 2020 John Wiley & Sons, Ltd
Detection of the β-lactoglobulin genotype in zebu cattle (Gangatiri) milk using high-resolution accurate mass spectroscopy
(Cambridge University Press, 2023) Manish Kumar Singh; Arvind Kumar; Ramadevi Nimmanapalli; Anand Kumar Pandey
We studied the genetic polymorphism of beta-lactoglobulin (β-Lg) whey protein in Gangatiri zebu cows for this Research Communication. The polymorphic nature of milk protein fractions and their association with milk production traits, composition and quality has attracted several efforts in evaluating the allelic distribution of protein locus as a potential dairy trait marker. Genetic variants of β-Lg have highly significant effects on casein number (B > A) and protein recovery (B > A) and also determine the yield of cheese dry matter (B > A). Molecular techniques of polyacrylamide gel electrophoresis and high-resolution accurate mass-spectroscopy were applied to characterize the β-Lg protein obtained from the Gangatiri breed milk. Sequence analysis of β-Lg showed the presence of variant B having UniProt database accession number P02754, coded on the PAEP gene. Our study can provide reference and guidance for the selection of superior milk (having β-LgB) from this indigenous breed that could potentially give a good yield of β-Lg for industrial applications. Copyright © The Author(s), 2023. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation.
Effect of nitrogen levels on growth attributes, yield and nutrient uptake of different rice (Oryza sativa l.) varieties under the transplanted condition
(Editorial board of Journal of Experimental Biology and Agricultural Sciences, 2021) Rakesh Maurya; Manoj Kumar Singh; Nikhil Kumar Singh; Manish Kumar Singh; Anurag Kumar Singh
Rice is very responsive to nitrogen fertilization under transplanted conditions; however N use efficiency and uptake by different varieties may vary. A field study was conducted during the rainy season of 2017 to assess the effect of four nitrogen levels in three rice varieties under the transplanted condition at Agricultural Research Farm, Banaras Hindu University, Varanasi, Uttar Pradesh. The experiment was carried out in a split-plot design (SPD) with three replications. The total number of treatments was twelve involving three rice varieties (V1-BPT-5204, V2-Rajendra Kasturi and V3-HUBR 2-1) in main plots and four nitrogen levels (N1-100, N2-120, N3-140, and N4-160 kg ha-1) in sub-plots. Rice variety BPT-5204 recorded significantly higher growth parameters viz. leaf number hill-1(37.17), SPAD value (chlorophyll content 31.83), the higher number of days taken to 50 percent flowering (103 days), maturity (130.17 days), grain yield (50.73 q ha-1), biological yield (112.90 q ha-1) and harvest index (0.45) as well as NPK uptakes by grain (60.96, 13.01 & 10.95 kg ha-1) and straw (29.72, 7.15 & 101.55 kg ha-1) as compared to other varieties. However, straw yield (62.17 q ha-1) was reported higher under HUBR 2-1 as compared to the rest varieties. Among the nitrogen levels, N4-160 kg N ha-1 was recorded significantly higher above the same parameter as compared to other nitrogen levels. Hence, the application of N @ 160 kg ha-1 along with rice variety BPT-5204 can be recommended for achieving higher rice yield, nutrient uptake, and efficiency in Eastern Uttar Pradesh. © 2021, Editorial board of Journal of Experimental Biology and Agricultural Sciences. All rights reserved.
Evaluation of anti-allergic and anti-anaphylactic activity of methanolic leaves extract of Chinese native shrub, Premna latifolia Roxb. in rodents
(Springer Science and Business Media Deutschland GmbH, 2025) Raj N. Kumar; Ashutosh Kumar; Brijesh Pawan Kumar; Manish Kumar Singh; Chandra Shekhar Azad
Premna latifolia Roxb. is used in a variety of traditional medicinal usages by the local community people of China and Southeast Asian regions to treat allergy and inflammatory diseases due to its great biodiversity. The anti-allergic efficacy of a methanolic extract of Premna latifolia Roxb. leaves was investigated in this study. The effect of Premna latifolia Roxb. extract at different doses (100, 200, And 300 mg/kg, p. o.) was evaluated on Animal models of asthma And allergy, such as milk-induced eosinophilia and leukocytosis, compound 48/80-induced mast cell degranulation, and active and passive cutaneous anaphylaxis. The impact of Premna latifolia Roxb. extract on sensitized guinea pig ileum and tracheal chain preparations was also studied. At various concentrations, treatment with Premna latifolia Roxb. extract significantly reduced (p value < 0.001) milk-induced eosinophilia, while stabilizing the compound 48/80-induced mast cell degranulation and lowering passive cutaneous and active anaphylactic reactions. Furthermore, Premna latifolia Roxb. extract prevented acetylcholine and histamine-induced tracheal chain contraction, as well as egg albumin-induced ileum contraction in sensitized guinea pigs (Shultz-Dale inhibition test). The anti-allergic and anti-anaphylactic effect of Premna latifolia Roxb. extract could be attributed to the stability of mast cells. Premna latifolia Roxb. showed anti-allergic and anti-anaphylactic properties at various doses, indicating that it is an effective phytomedicine for treating such illnesses. © The Author(s), under exclusive licence to Springer-Verlag London Ltd., part of Springer Nature 2025.
Foodomics-based metabolites profiling of the Greek yogurt incorporated with unripened papaya peel powder
(Elsevier B.V., 2024) Sohan Lal Bajya; Durga Shankar Bunkar; Sunil Kumar Goyal; Manish Kumar Singh; Vinod Kumar Paswan; Shankar Lal; Priya Dhyani
The food waste of the fruit processing industry is rich in many bio-active components such as polysaccharides, polyphenols, peptides, etc. that own multifaceted health benefits. The valorization of this waste is an intriguing optimization method for various dairy products. Meanwhile, LC-MS-based foodomics has been an emerging approach for the quantitative and qualitative analysis of dairy foods. Untargeted metabolomics has been done of the optimized functional yogurt that contains different levels of unripened papaya peel powder (UPPP) using high-resolution mass spectroscopy for analysis of added bio-active components in the matrix. UPPP comprises a high content of phytochemicals which could give functionality and therapeutic effect to the Greek yogurt. A total of 36 functional metabolites have been identified which have various health-beneficial attributes. Kaempferol, ostruthin, putative carpaine derivatives, etc. are some of the metabolites of high importance with a wide area coverage in the metabolome. This work highlights the bioactivity of the UPPP and its prebiotic properties added to the functional yogurt as an independent ingredient. The incorporated plant-based ingredients like UPPP can effectively enhance the functional attributes of Greek yogurt, which is a potential synbiotic food. © 2024 The Author(s)
High-resolution mass spectrometer-based identification of β-Casein variant (A2/A1) in the milk of Indian Holstein Friesian crossed cows
(Academic Press Inc., 2024) Manish Kumar Singh; Arvind Kumar; Ramadevi Nimmanapalli; Mohit Malik; Ankur Aggarwal; Vishal Kumar; Mahendra Kumar
Foodomics tools are ameliorating the compositional and quality analysis part of various domains of food products. The use of proteomics in the polymorphic product investigation of beta-casein (β-Cn) is intriguing because of the fast and reliable results from the accurate mass spectrometry. This study delineates the LC-MS/MS-based sequential analysis of gel fractionated β-Cn of eighteen Holstein Friesian (HF) crossed cow milk for the identification of A2 milk. The probe of genetically induced polymorphism of β-Cn milk protein in the crossed animals is essential for the quality concern and fulfilling the demand of the consumers. Several in-vivo studies have shown that proteolytic digestion of the A1 milk secreted an opioid, i.e., β-casomorphin-7 that can cause many types of non-communicable diseases. SDS-PAGE was done on 15% resolving gel and an orbitrap mass spectrometer equipped with UniProt database search was used for the detection of the genetic variants in the HF crossed cows have shown ten animals are lactating milk with A2 β-Cn (Pro67), whereas only eight animals have A1 β-Cn (His67). The high number of HF-crossed cows are producing A2 milk. Among the molecular biology methods, top-down proteomics is the most efficient, accurate, and sustainable technique of milk protein genetic variant analysis. © 2024 Elsevier Inc.
Identification of β-casein phenotypes (A1/A2) in the milk of the Indian Jersey crossbreed bovine using the high-resolution accurate mass spectrometer
(John Wiley and Sons Inc, 2024) Manish Kumar Singh; Arvind Kumar; Dinesh Chandra Rai; Ankur Aggarwal; Mohit Malik
Foodomics is an emerging probing method of phenotype investigation of the different milk proteins and their subtypes. The polymorphic nature of the β-casein (β-Cn) protein has shown fourteen different protein variants to date in bovines. The analysis of the β-Cn genetic polymorphism from the milk of the crossbred dairy animals is crucial for the quality assurance of the consumers from the various health concerns, especially those linked with the A1 phenotype which yields β-casomorphin-7 on in vivo digestion. Jersey-crossed Indian cattle have been widely utilised in dairy because of their better milk production and survival performance trait. In this investigation, an SDS-PAGE coupled with a high-resolution accurate mass spectrometry-based proteomics approach has been applied to identify the presence of specific phenotype of the β-Cn protein in the milk of the 24 Indian crossbred (Jersey crossed) animals. Amino acid sequential analysis has been done using different search modules, as MS Amanda and Sequest HT showed 17 cows are producing A2 β-Cn (Pro~67) while only seven animals yielded the A1 variant (His~67). The maximum number of Indian Jersey-crossed animals are lactating milk having A2 β-Cn. The A2 milk from the crossbred animals is free from the negative impact on health caused by β-casomorphin-7 (BCM-7) released during digestion of the A1 phenotype. Among the molecular biology techniques, top-down proteomics has been an intriguing technique for the identification of protein genetic polymorphic products. © 2023 Institute of Food, Science and Technology (IFSTTF).
Impact of farm sector electricity subsidy on water use efficiency and water productivity in India
(Indian Society of Agricultural Economics, 2014) O.P. Singh; Rakesh Singh; Manish Kumar Singh
Irrigation plays an important role in the growth of India agriculture and it helped the farmers to grow multiple crops during the year. The share of groundwater in total net irrigated area (20.85 million hectares) increased by 28.67 per cent to 61.40 per cent during 1950-51 to 2010-11. Out of the total net irrigated area in the country, the share of groundwater irrigated area was 28.67 per cent and 61.40 per cent in 1950-51 and 2010-11 respectively. The growth in net area irrigated by groundwater is growing at a compound growth rate of 3.54 per cent per annum during 1950-51 to 2010-11. The total electric operated pump for pumping groundwater in the country increased from 10.27 million to 11.05 million during 2001 to 2006 whereas, the diesel operated pumps declined from 6.55 million to 6.30 million during 2001 to 2006. The electric subsidy to farm sector has increased from Rs 7334.9 crore to Rs 45561.0 crore during 1992-93 to 2011-12 registering a compound growth rate of 8.65 per cent per annum. The present study has tried to assess the impact of electricity on groundwater use and water use efficiency. The study was confined to three Indian states viz., Urtar Pradesh, Bihar and Punjab. In case of Urtar Pradesh and Bihar, diesel pump owners were using less irrigation water for all the crops as compared to electric pump owners. The diesel pump owners were getting the highest physical and economic water productivity for all the crops except paddy as compared to electric pump owners. In case of Punjab, farmers were using less groundwater for all the crops as compared to canal water. The physical water productivity was higher for all the crops grown under groundwater irrigation except bajra and barseem. The net economic value of water productivity was found higher for groundwater irrigated field in maize, bajra and wheat. The study suggests that the introduction of pro-rata pricing of electricity supply to farm sector could be the best option for better management of groundwater. This would help in equitable, efficient and sustainable use of groundwater and reduce the burden of gigantic electricity subsidy to farm sector. Pre-paid metering to farm sector may also increase water use efficiency.
Investigating NAC Transcription Factor Role in Redox Homeostasis in Solanum lycopersicum L.: Bioinformatics, Physiological and Expression Analysis under Drought Stress
(MDPI, 2022) Nagendra Rai; Krishna Kumar Rai; Manish Kumar Singh; Jagdish Singh; Prashant Kaushik
NAC transcription factors regulate stress-defence pathways and developmental processes in crop plants. However, their detailed functional characterization in tomatoes needs to be investigated comprehensively. In the present study, tomato hybrids subjected to 60 and 80 days of drought stress conditions showed a significant increase in membrane damage and reduced relative water, chlorophyll and proline content. However, hybrids viz., VRTH-16-3 and VRTH-17-68 showed superior growth under drought stress, as they were marked with low electrolytic leakage, enhanced relative water content, proline content and an enhanced activity of enzymatic antioxidants, along with the upregulation of NAC and other stress-defence pathway genes. Candidate gene(s) exhibiting maximum expression in all the hybrids under drought stress were subjected to detailed in silico characterization to provide significant insight into its structural and functional classification. The homology modelling and superimposition analysis of predicted tomato NAC protein showed that similar amino acid residues were involved in forming the conserved WKAT domain. DNA docking discovered that the SlNAC1 protein becomes activated and exerts a stress-defence response after the possible interaction of conserved DNA elements using Pro72, Asn73, Trp81, Lys82, Ala83, Thr84, Gly85, Thr86 and Asp87 residues. A protein–protein interaction analysis identified ten functional partners involved in the induction of stress-defence tolerance. © 2022 by the authors.
Lanthanide doped ultrafine hybrid nanostructures: Multicolour luminescence, upconversion based energy transfer and luminescent solar collector applications
(Royal Society of Chemistry, 2017) Priyam Singh; Praveen Kumar Shahi; Sunil Kumar Singh; Akhilesh Kumar Singh; Manish Kumar Singh; Rajiv Prakash; Shyam Bahadur Rai
We herein demonstrate novel inorganic-organic hybrid nanoparticles (HNPs) composed of inorganic NPs, NaY0.78Er0.02Yb0.2F4, and an organic β-diketonate complex, Eu(TTA)3Phen, for energy harvesting applications. Both the systems maintain their core integrity and remain entangled through weak interacting forces. HNPs incorporate the characteristic optical behaviour of both the systems i.e. they give an intense red emission under UV excitation, due to Eu3+ in organic complexes, and efficient green upconversion emission of Er3+ in inorganic NPs for NIR (980 nm) excitation. However, (i) an energy transfer from Er3+ (inorganic NPs) to Eu3+ (organic complex) under NIR excitation, and (ii) an increase in the decay time of 5D0 → 7F2 transition of Eu3+ for HNPs as compared to the Eu(TTA)3Phen complex, under different excitation wavelengths, are added optical characteristics which point to an important role of the interface between both the systems. Herein, the ultra-small size (6-9 nm) and spherical shape of the inorganic NPs offer a large surface area, which improves the weak interaction force between both the systems. Furthermore, the HNPs dispersed in the PMMA polymer have been successfully utilized for luminescent solar collector (LSC) applications. © The Royal Society of Chemistry 2017.
Metabolomics and microscopic profiling of flaxseed meal- incorporated Peda
(Elsevier B.V., 2024) Sachin Maurya; Tarun Verma; Ankur Aggarwal; Manish Kumar Singh; Abhishek Dutt Tripathi; Ankur Trivedi
Functional dairy foods are in high demand due to their convenience, enhanced nutrition, intriguing flavors, and natural ingredients. The valorization of flaxseed by-products can potentially boost the functionality of these foods. This work involves the optimization of flaxseed meal powder (2%, 2.5%, 3%) during Peda preparation based on sensory and textural attributes. The optimized Peda (2%) exhibited significantly reduction in moisture (39.6%) and water activity (18.9%), while significantly increasing crude fiber (1.88%), protein (26.4%), fat (8%) and DPPH inhibition (274.5%) as compared to control Peda. Scanning electron microscopy of the optimized Peda revealed the surface displayed a dense, uneven texture, heavily coated with fat, and intergranular spaces filled with milk serum. Twenty-three primary compounds were recognized in high-resolution mass spectrometry (HR-MS), including 6 organic acids, 6 amino acids, 3 fatty acids, 3 other metabolite derivatives, 2 lipids, 2 bioactive components, and 1 sugar. Besides gas chromatography mass spectrometry (GC–MS) found six separate types of fatty acids. These compounds have been proven to possess various bioactivities, such as promoting brain activity, antioxidant, anti-diabetic, anti-inflammatory, cardiovascular-protective effects, etc. Flaxseed meal, as a plant-based substitute for dairy ingredients, offers a sustainable and healthy alternative, making flaxseed-incorporated Peda a functional food. © 2024 The Author(s)
Molecular characterization and expression analysis of hsp60 gene homologue of sheep blowfly, Lucilia cuprina
(Elsevier Ltd, 2015) Manish Kumar Singh; P.V. Janardhan Reddy; A.S. Sreedhar; P.K. Tiwari
The 60 kDa heat shock protein (Hsp60) or chaperonin is one among the highly conserved families of heat shock proteins, known to be involved in variety of cellular activities, including protein folding, thermal protection, etc. In this study we sequence characterized hsp60 gene homologue of Lucilia cuprina, isolated and cloned from the genomic library as well as by genomic PCR, followed by RACE- PCR. The L. cuprina hsp60 gene/protein expression pattern was analyzed in various tissues, either at normal temperature (25±1 °C) or after exposure to heat stress (42 °C). The analysis of nucleotide sequence of Lchsp60 gene revealed absence of intron and the nuclear localizing signal (NLS). The deduced amino acid sequence showed presence of unique conserved sequences, such as those for mitochondrial localization, ATP binding, etc. Unlike Drosophila, Lucilia showed presence of only one isoform, i.e., hsp60A. Phylogenetic analysis of hsp60 gene homologues from different species revealed Lchsp60 to have >88.36% homology with D. melanogaster, 76.86% with L. sericata, 58.31% with mice, 57.99% with rat, and 57.72% with human. Expression analysis using Real Time PCR and fluorescence imaging showed significant enhancement in the expression level of Lchsp60 upon heat stress in a tissue specific manner, indicating its likely role in thermo-tolerance as well as in normal cellular activities. © 2015 Elsevier Ltd.
Political competition and environment quality: a study of India
(Springer Science and Business Media Deutschland GmbH, 2023) Yadawananda Neog; Manish Kumar Singh; Anup Kumar Yadava; Achal Kumar Gaur
The focus of sustainable development goals (SDGs) is to promote the use of renewable energy so that countries can achieve better environmental quality. However, the progression is plodding, and still, 80% of energy comes mainly from conventional sources in developing countries. The implementation of procedures depends on the political attitudes, political stability, and quality of institutions. India has a diverse political structure ranging from central government to state government to local governments. In the late ‘80 s, India witnessed a stiff rise in regional and national political parties, which leads more political competition. This paper tries to explain the possible relationship between political competition and CO2 emission in India. With the application of the time series non-linear ARDL (NARDL) model, this study tries to find the asymmetric relationship between political competition and CO2 emission. In our empirical model, we also include other important elements of environmental quality like innovation and fossil fuel consumption. Empirical results show that political competition is asymmetrically related to CO2 emissions in the long run. Fossil fuel consumption and innovation also have a significant relationship with emissions. Based on the results, a few policy recommendations have been discussed. © 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
Probing of alpha, beta and kappa-caseins polymorphic variants in Gangatiri cow milk with the use of polyacrylamide gel electrophoresis and HRAMS
(Cambridge University Press, 2023) Manish Kumar Singh; Arvind Kumar; Ramadevi Nimmanapalli; Ankita Hooda
This research communication aimed to probe the genetic polymorphisms of alpha, beta, and kappa caseins in Gangatiri cows (an indigenous Indian cattle). Detection of variants has received considerable research interest in the dairy industry and animal breeding in recent years as a source of good quality protein, but also of bioactive peptides that may be linked to health implications. The polymorphic nature of casein fractions and their association with milk production traits, composition, and quality also attracted several efforts in evaluating the allelic distribution of different casein locus as a potential dairy trait marker. Molecular techniques of polyacrylamide gel electrophoresis and high-resolution accurate mass-spectrometry have been applied to this probe. Sequence analysis of different casein types in the cows showed the presence of four specific variants. Copyright © The Author(s), 2023. Published by Cambridge University Press on behalf of Hannah Dairy Research Foundation.
Proteomics-based milk whey proteome profiling of Indian Jersey crossbreed cows followed by chromosomal mapping
(John Wiley and Sons Ltd, 2023) Manish Kumar Singh; Arvind Kumar; Ramadevi Nimmanapalli; Anand Kumar Pandey
BACKGROUND: Milk contains a massive class of minor proteins that are known for their various biological and molecular functions. Many whey proteins transfer the host defense mechanism to the human body. In this assay, electrophoresis followed by a high-resolution mass spectrometry-based proteomic approach has been applied to identify the whey proteome of Indian Jersey crossbreed bovines. RESULTS: Two search engines, MS Amanda and Sequest HT, have shown more than 29 minor proteins. Chromosomal mapping revealed that chromosomes 5 and 9 are expressing maximum proteins in the whey proteome. The principal component analysis, outlier plots, scree plots, score plots, and loading plots were generated to further assess the results. CONCLUSION: The majorly expressed ones are glycosylation-dependent cell adhesion molecule-1, ubiquitin, desmoglein, annexin, glycoprotein, arginase, histones, peroxiredoxin, vimentin, desmin, catenin, peripherin, and 70 kDa heat shock protein. © 2023 Society of Chemical Industry. © 2023 Society of Chemical Industry.