Browsing by Author "Manish Srivastava"

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Algal biohydrogen production: Impact of biodiversity and nanomaterials induction
(Elsevier Ltd, 2023) Tripti Singh; Anisha Sehgal; Rajeev Singh; Shalini Sharma; Dan Bahadur Pal; Hanaa M. Tashkandi; Rajaa Raddadi; Steve Harakeh; Shafiul Haque; Manish Srivastava; Ashraf Aly Hassan; Neha Srivastava; Vijai Kumar Gupta
Fossil fuels are limited in nature and are not environmentally friendly, thus using them to meet the rising energy needs is insufficient. Another major cause of global warming, which is recognized as one of the greatest hazards to the world, is fossil fuels. Finding alternative energy sources that can counteract the drawbacks of fossil fuels is urgently needed. Due to its low environmental impact and a variety of possible sustainable production methods, biohydrogen is one such alternative energy source that has attracted enormous interest and demand. Due to their wide range of environments, rapid growth, and polyphyletic nature, algae-based biological hydrogen production techniques are gaining significant interest. Nevertheless, the main obstacles to the sustainable and commercial application of the algal biohydrogen generation process are low yield, constrained light penetration, low biomass concentration, and expensive downstream processes. Increased attention to algal diversity may help to overcome the limitation of low algal biomass production and yield while enhancing penetration ability. Additionally, the usage of nanomaterials may speed up the process by altering the entire process' response mechanism. Therefore, this review explores algal diversity as one of the strategies of algal biohydrogen production along with elaboration of the impacts of nanomaterials in different pathways of biohydrogen production, namely dark fermentation, photo-fermentation, direct and indirect biophotolysis. Advances in biohydrogen production employing diversified groups of algae with the application of nanomaterials have been extensively summarized with current update mechanisms and existing roadblocks. As a result, the utilization of nanomaterials as a novel and sustainable catalyst has also been thoroughly described for prospective scaling up of algal biohydrogen production. © 2023 Elsevier Ltd
Bacterial cellulase production via co-fermentation of paddy straw and Litchi waste and its stability assessment in the presence of Zn–Mg mixed-phase hydroxide-based nanocomposite derived from Litchi chinensis seeds
(Elsevier B.V., 2023) Mohammed Asiri; Tripti Singh; Akbar Mohammad; Amer Al Ali; Abdulaziz Alqahtani; Mohd Saeed; Manish Srivastava
Co-fermentation via co-cultured bacterial microorganisms to develop enzymes in solid-state fermentation (SSF) is a promising approach. This strategy is imperative in a series of sustainable and effective approaches due to superior microbial growth and the use of a combination of inexpensive feedstocks for enzyme production wherein mutually participating enzyme-producing microbial communities are employed. Moreover, the addition of nanomaterials to this technique may aid in its prominent advantage of enhancing enzyme production. This strategy may be able to decrease the overall cost of the bioprocessing to produce enzymes by further implementing biogenic, route-derived nanomaterials as catalysts. Therefore, the present study attempts to explore endoglucanase (EG) production using a bacterial coculture system by employing two different bacterial strains, namely, Bacillus subtilis and Serratia marcescens under SSF in the presence of a Zn–Mg hydroxide-based nanocomposite as a nanocatalyst. The nanocatalyst based on Zn–Mg hydroxide has been prepared via green synthesis using Litchi waste seed, while SSF for EG production has been conducted using cofermentation of litchi seed (Ls) and paddy straw (Ps) waste. Under an optimized substrate concentration ratio of 5:6 Ps:Ls and in the presence of 2.0 mg of nanocatalyst, the cocultured bacterial system produced 1.6 IU/mL of EG enzyme, which was ~1.33 fold higher as compared to the control. Additionally, the same enzyme showed its stability for 135 min in the presence of 1.0 mg of nanocatalyst at 38 °C. The nanocatalyst has been synthesized using the green method, wherein waste litchi seed is used as a reducing agent, and the nanocatalyst could be employed to improve the production and functional stability of crude enzymes. The findings of the present study may have significant application in lignocellulosic-based biorefinaries and cellulosic waste management. © 2023 Elsevier B.V.
Biogenic synthesis of CuO/ZnO nanocomposite from Bauhinia variegate flower extract for highly sensitive electrochemical detection of vitamin B2
(Elsevier Ltd, 2024) Diksha Singh; Rahul Verma; Kshitij RB Singh; Manish Srivastava; Ravindra Pratap Singh; Jay Singh
In this study, we report the preparation of bio-inspired binary CuO/ZnO nanocomposite (bb-CuO/ZnO nanocomposite) via the biological route using Bauhinia variegata flower extract following hydrothermal treatment. The prepared bb-CuO/ZnO nanocomposite was electrophoretically deposited (EPD) on indium tin oxide (ITO) substrate to develop bb-CuO/ZnO/ITO biosensing electrode which is employed for the determination of vitamin B2 (Riboflavin) through electrochemical techniques. Physicochemical assets of the prepared bb-CuO/ZnO nanocomposite have been extensively evaluated and make use of different characterization techniques including powder XRD, FT-IR, AFM, SEM, TEM, EDX, XPS, Raman, and TGA. Electrochemical characteristics of the bb-CuO/ZnO/ITO biosensing electrode have been studied towards vitamin B2 determination. Furthermore, different biosensing parameters such as response time, reusability, stability, interference, and real sample analysis were also estimated. From the linear plot of scan rate, charge transfer rate constant (Ks), surface concentration of electrode (γ), and diffusion coefficient (D) have been calculated, and these are found to be 6.56 × 10−1 s−1, 1.21 × 10−7 mol cm−2, and 6.99 × 10−3 cm2 s−1, respectively. This biosensor exhibits the linear range of vitamin B2 detection from 1 to 40 μM, including sensitivity and limit of detection (LOD) of 1.37 × 10−3 mA/μM cm2 and 0.254 μM, respectively. For higher concentration range detection linearity is 50–100 μM, with sensitivity and the LOD of 1.26 × 10−3 mA/μM cm2 and 0.145 μM, respectively. The results indicate that the bio-inspired nanomaterials are promising sustainable biosensing platforms for various food and health-based biosensing devices. © 2024 Elsevier B.V.
Bioinspired fabrication of zinc hydroxide-based nanostructure from lignocellulosic biomass Litchi chinensis leaves and its efficacy evaluation on antibacterial, antioxidant, and anticancer activity
(Elsevier B.V., 2023) Manish Srivastava; Kshitij RB Singh; Tripti Singh; Mohammed Asiri; Muath Suliman; Haleema Sabia; Prakash Ranjan Deen; Radha Chaube; Jay Singh
Zinc-based nanostructures are known for their numerous potential biomedical applications. In this context, the biosynthesis of nanostructures using plant extracts has become a more sustainable and promising alternative to effectively replace conventional chemical methods while avoiding their toxic impact. In this study, following a low-temperature calcination process, a green synthesis of Zn-hydroxide-based nanostructure has been performed using an aqueous extract derived from the leaves of Litchi chinensis, which is employed as a lignocellulose waste biomass known to possess a variety of phytocompounds. The biogenic preparation of Zn-hydroxide based nanostructures is enabled by bioactive compounds present in the leaf extract, which act as reducing and capping agents. In order to evaluate its physicochemical characteristics, the produced Zn-hydroxide-based nanostructure has been subjected to several characterization techniques. Further, the multifunctional properties of the prepared Zn-hydroxide-based nanostructure have been evaluated for antioxidant, antimicrobial, and anticancer activity. The prepared nanostructure showed antibacterial efficacy against Bacillus subtilis and demonstrated its anti-biofilm activity as evaluated through the Congo red method. In addition, the antioxidant activity of the prepared nanostructure has been found to be dose-dependent, wherein 91.52 % scavenging activity could be recorded at 200 μg/ml, with an IC50 value of 45.22 μg/ml, indicating the prepared nanostructure has a high radical scavenging activity. Besides, the in vitro cytotoxicity investigation against HepG2 cell lines explored that the as-prepared nanostructure exhibited a higher cytotoxic effect and 73.21 % cell inhibition could be noticed at 25.6 μg/ml with an IC50 of 2.58 μg/ml. On the contrary, it was found to be significantly lower in the case of HEK-293 cell lines, wherein ~47.64 % inhibition could be noticed at the same concentration. These findings might be further extended to develop unique biologically derived nanostructures that can be extensively evaluated for various biomedical purposes. © 2023 Elsevier B.V.
CISS-Based Label-Free Novel Electrochemical Impedimetric Detection of UVC-Induced DNA Damage
(American Chemical Society, 2022) Neeraj Bangruwa; Manish Srivastava; Debabrata Mishra
In this work, we demonstrate chiral-induced spin selectivity (CISS)-based label-free electrochemical impedimetric detection of radiation-induced DNA damage using the electrons' spin as a novel tool of sensing. For this, self-assembled monolayers (SAMs) of short ds-DNA (of length 7.14 nm) are prepared on arrays of multilayer thin film devices comprising a gold overlay (500 μm diameter with 10 nm thickness) on a nickel thin film (100 nm) fabricated by the physical vapor deposition technique. Subsequently, the SAMs of ds-DNA are exposed to ultraviolet C (UVC) radiation for a prolonged period of 8 h to induce structural perturbations in DNA. The susceptibility of DNA to radiation-induced damage was probed by recording the spin-dependent electrochemical impedimetric spectra, wherein a continuous sinusoidal wave of the amplitude of 10 mV was superimposed on DC bias in the frequency range of 100-105 Hz, with simultaneous spin injection through the attached DNA. The inherent correlation between the charge-transfer resistance (Rct) and the spin selectivity of electrons through DNA was taken into account for the detection of DNA damage for the first time with a limit of detection achieved up to 10 picomolar concentrations of DNA. As the spin-polarized electrons directly probe the structural symmetry, it is robust against perturbation from electronic signals usually found in conventional electrochemical biosensors. © 2016 American Chemical Society.
Controlled synthesis and magnetic properties of monodispersed ceria nanoparticles
(American Institute of Physics Inc., 2015) Sumeet Kumar; Manish Srivastava; Jay Singh; Samar Layek; Madhu Yashpal; Arnulf Materny; Animesh K. Ojha
In the present study, monodispersed CeO2 nanoparticles (NPs) of size 8.5 ± 1.0, 11.4 ± 1.0 and 15.4 ± 1.0 nm were synthesized using the sol-gel method. Size-dependent structural, optical and magnetic properties of as-prepared samples were investigated by X-ray diffraction (XRD), field emission scanning electron microscope (FE-SEM), high resolution transmission electron microscopy (HR-TEM), ultra-violet visible (UV-VIS) spectroscopy, Raman spectroscopy and vibrating sample magnetometer (VSM) measurements. The value of optical band gap is calculated for each particle size. The decrease in the value of optical band gap with increase of particle size may be attributed to the quantum confinement, which causes to produce localized states created by the oxygen vacancies due to the conversion of Ce4+ into Ce3+ at higher calcination temperature. The Raman spectra showed a peak at ∼461 cm-1 for the particle size 8.5 nm, which is attributed to the 1LO phonon mode. The shift in the Raman peak could be due to lattice strain developed due to variation in particle size. Weak ferromagnetism at room temperature is observed for each particle size. The values of saturation magnetization (Ms), coercivity (Hc) and retentivity (Mr) are increased with increase of particle size. The increase of Ms and Mr for larger particle size may be explained by increase of density of oxygen vacancies at higher calcination temperature. The latter causes high concentrations of Ce3+ ions activate more coupling between the individual magnetic moments of the Ce ions, leading to an increase of Ms value with the particle size. Moreover, the oxygen vacancies may also produce magnetic moment by polarizing spins of f electrons of cerium (Ce) ions located around oxygen vacancies, which causes ferromagnetism in pure CeO2 samples. © 2015 Author(s).
Efficient energy storage performance of in situ grown Co3V2O8-RGO composite nanostructure for high performance asymmetric Co3V2O8-RGO//RGO supercapacitors and consequence of magnetic field induced enhanced capacity
(Elsevier Ltd, 2021) Pooja Devi; Manish Srivastava; Nam Hoon Kim; Joong Hee Lee; Debabrata Mishra
In this work, cobalt vanadate/reduced graphene oxide (Co3V2O8/RGO) composite nanostructure has been synthesized via in-situ reduction of graphene oxide (GO) in the presence of cobalt chloride and sodium metavanadate through hydrothermal following post calcinations treatment. Characterizations through various techniques have been performed to probe the physicochemical properties of Co3V2O8/RGO, RGO and Co3V2O8 nanostructures. Our Results show that because of the synergistic effect, Co3V2O8/RGO composite nanostructure exhibits superior electrochemical properties as compared to bare RGO and Co3V2O8. At a current density of 0.5 A/g the specific capacity has been recorded to be 118.82, ∼179, and ∼241.67 Cg-1 in case of Co3V2O8, RGO and Co3V2O8/RGO nanostructures, respectively. Moreover, Co3V2O8/RGO composite nanostructure (positive electrode) and RGO (negative electrode) have been employed to prepare the asymmetric supercapacitor, exhibited high specific capacity (127.62Cg-1), energy density (28.36 Whkg−1), and power density (400 Wkg-1) at a current density 0.5 A/g. This asymmetric supercapacitor shows excellent capacity retention (∼91.64%) and columbic efficiency (∼98.61%) measured at a current density of 5 A/g after 10,000 charge/discharge cycles. More importantly, a dramatic increase ∼170% (344 Cg-1) and ∼67% (185 Cg-1) in the specific capacity have been recorded at a current density of 0.5 A/g and 1.0 A/g, respectively when asymmetric supercapacitor device is subjected to an external magnetic field of strength of 0.5 T. Additionally, under the applied magnetic field, capacity retention ∼97.75% and columbic efficiency ∼98.84% has been recorded at a current density of 5 A/g after 10,000 cycles. © 2021 Elsevier Ltd
Fungal Cellulases Production for Biodegradation of Agriculture Waste
(Springer, 2018) Neha Srivastava; Manish Srivastava; Ambepu Manikanta; P.W. Ramteke; Ram Lakhan Singh; P.K. Mishra; S.N. Upadhyay
Cellulases have several existing and potential applications in various industries including biofuel, pulp and paper, detergent, juice, and textile. Different enzyme components found in the cellulase system can effectively depolymerize the cellulose into monomeric sugars. Fungi are regarded as an ideal candidate to produce a complete cellulase system. Cellulase production via solid-state fermentation (SSF) using fungi is one of the most desirable and cost-effective routes. The present chapter provides an outline on the major steps of fungal cellulase production using SSF. This chapter presents a concise account of fungal cellulases as well as their production and key role in bioconversion of lignocellulosic waste. Various aspects of fungal cellulase production using the agriculture wastes and their components have been discussed. Additionally, potential fungal sources of cellulase along with effective bioconversion of agricultural biomass are also summarized. © Springer Nature Singapore Pte Ltd. 2018.
Light-Amplified CISS-Based Hybrid QD-DNA Impedimetric Device for DNA Hybridization Detection
(American Chemical Society, 2023) Prashant K. Bhartiya; None Suryansh; Neeraj Bangruwa; Manish Srivastava; Debabrata Mishra
We design and build a novel light-amplified electrochemical impedimetric device based on the CISS effect to detect DNA hybridization using a hybrid quantum dot (QD)-DNA monolayer on a ferromagnetic (FM) Ni/Au thin film for the first time. Using spin as a detection tool, the current research considers the chiral-induced spin selectivity (CISS) phenomenon. After injecting a spin current into the QD-DNA system with opposite polarities (up and down), the impedimetric device revealed a large differential change in the charge-transfer resistance (ΔRct) of ∼100 ohms for both spins. Nearly, a threefold increase in the ΔRct value to ∼270 ohms is observed when light with a wavelength of 532 nm is illuminated on the sample, owing to the amplified CISS effect. The yield of spin polarization as extracted from the Nyquist plot increases by a factor of more than 2 when exposed to light, going from 6% in the dark to 13% in the light. The impact of light on the CISS effect was further corroborated by the observation of the spin-dependent asymmetric quenching of photoluminescence (PL) in the same hybrid system. These observations are absent in the case of a noncomplementary QD-DNA system due to the absence of a helical structure in DNA. Based on this, we develop a spin-based DNA hybridization sensor and achieve a limit of detection of 10 fM. These findings open a practical path for the development of spin-based next-generation impedimetric DNA sensors and point-of-care devices. © 2023 American Chemical Society
Microbial beta glucosidase enzymes: Recent advances in biomass conversation for biofuels application
(MDPI AG, 2019) Neha Srivastava; Rishabh Rathour; Sonam Jha; Karan Pandey; Manish Srivastava; Vijay Kumar Thakur; Rakesh Singh Sengar; Vijai K. Gupta; Pranab Behari Mazumder; Ahamad Faiz Khan; Pradeep Kumar Mishra
The biomass to biofuels production process is green, sustainable, and an advanced technique to resolve the current environmental issues generated from fossil fuels. The production of biofuels from biomass is an enzyme mediated process, wherein β-glucosidase (BGL) enzymes play a key role in biomass hydrolysis by producing monomeric sugars from cellulose-based oligosaccharides. However, the production and availability of these enzymes realize their major role to increase the overall production cost of biomass to biofuels production technology. Therefore, the present review is focused on evaluating the production and efficiency of β-glucosidase enzymes in the bioconversion of cellulosic biomass for biofuel production at an industrial scale, providing its mechanism and classification. The application of BGL enzymes in the biomass conversion process has been discussed along with the recent developments and existing issues. Moreover, the production and development of microbial BGL enzymes have been explained in detail, along with the recent advancements made in the field. Finally, current hurdles and future suggestions have been provided for the future developments. This review is likely to set a benchmark in the area of cost effective BGL enzyme production, specifically in the biorefinery area. © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
Novel conducting lithium ferrite/chitosan nanocomposite: Synthesis, characterization, magnetic and dielectric properties
(Elsevier, 2014) Manish Srivastava; Jay Singh; Rajneesh K. Mishra; Manish K. Singh; Animesh K. Ojha; Madhu Yashpal; Srivastava Sudhanshu
A study on Lithium ferrite/chitosan nanocomposite (LFCN), easily moldable into arbitrary shapes, as the conducting polymer and ferromagnetic characteristics is presented. The composite material is produced in the presence of Li0.5Cr0.1Fe2.4O4 and Li 0.5Co0.1Fe2.4O4 nanoparticle by ex-situ polymerizations process. Various characterizations techniques have been used to explore the characteristic of the synthesized products. The frequency dependent dielectric properties and electrical conductivity of all the samples have been measured through complex impedance plot in the frequency range of 1 kHz-6 MHz at room temperature. It was observed that in case of (LFCN), fluctuation in value of (ε′) and (ε″) is ceased over the frequency range of 4 Mz which can be attributed to the steady storage and dissipation of energy in the nanocomposite system. Moreover, it is also observed that electrical conductivity of (LFCN) increases with frequency and its value was found to be (0.032-0.048) (ohm-cm)-1 in frequency range of 1 kHz-6 MHz. Due to its low cost, a simple synthesis process and high flexibility, the proposed LFCN may find applications in various types of electronic components. © 2014 Elsevier B.V. All rights reserved.
Sustainable approaches towards green synthesis of TiO2 nanomaterials and their applications in photocatalysis-mediated sensing to monitor environmental pollution
(John Wiley and Sons Ltd, 2023) Tripti Singh; Shalini Sharma; Rajeev Singh; Dan Bahadur Pal; Irfan Ahmad; Mohammad Mahtab Alam; Nand Lal Singh; Manish Srivastava; Neha Srivastava
Nanomaterials are gaining enormous interests due to their novel applications that have been explored nearly in every field of our contemporary society. In this scenario, preparations of nanomaterials following green routes have attracted widespread attention in terms of sustainable, reliable, and environmentally friendly practices to produce diverse nanostructures. In this review, we summarize the fundamental processes and mechanisms of green synthesis approaches of TiO2 nanoparticles (NPs). We explore the role of plants and microbes as natural bioresources to prepare TiO2 NPs. Particularly, focus has been made to explore the potential of TiO2-based nanomaterials to design a variety of sensing platforms by exploiting the photocatalysis efficiency under the influence of a light source. These types of sensing are of massive importance for monitoring environmental pollution and therefore for inventing advanced strategies to remediate hazardous pollutants and offer a clean environment. © 2022 John Wiley & Sons Ltd.
Synthesis of superparamagnetic bare Fe 3O 4 nanostructures and core/shell (Fe 3O 4/alginate) nanocomposites
(2012) Manish Srivastava; Jay Singh; Madhu Yashpal; Dinesh Kumar Gupta; R.K. Mishra; Shipra Tripathi; Animesh K. Ojha
In this article we report about the synthesis of superparamagnetic bare Fe 3O 4 nanostructures and core/shell (Fe 3O 4/alginate) nanocomposites by simple low-temperature based method at pH values 5, 9, and 14. The structural morphology and magnetic behavior of Fe 3O 4 nanostructures and core/shell (Fe 3O 4/alginate) nanocomposites (Fe 3O 4/alg NCs) have been investigated by X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy (RS), ultraviolet-visible (UV-vis) spectroscopy, transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDX) and vibrating sample magnetometer (VSM). The particle size was calculated by TEM measurements and it turns out to be ∼10 nm and ∼14 nm for bare Fe 3O 4 nanoparticle and Fe 3O 4/alg NCs with core/shell structure, respectively. The magnetic properties of the synthesized products were found to be function of pH at which the synthesis has been done. The synthesized Fe 3O 4 nanoparticle and Fe 3O 4/alg NCs were found to be superparmagnetic in nature at room temperature. We observed that the value of saturation magnetization in case of Fe 3O 4/alg NCs decreases by increasing the pH value. © 2012 Elsevier Ltd. All rights reserved.
Synthesis, growth mechanism and characterization of single crystalline λ-Fe 2o 3 Spherical Nanoparticles
(2012) Manish Srivastava; Jay Singh; Madhu Yashpal; Animesh K. Ojha
In the present report, we proposed a simple and efficient method for synthesizing single crystalline-Fe 2o 3 spherical nanoparticles array into hexagonal dipyramid (HGDP) hierarchical structures using urea as a surface-active agent to control the growth and nucleation of the iron species. Growth mechanisms for the formation of HGDP hierarchical structures have been also proposed. Single crystalline feature, structural morphology and size of the nanoparticles were investigated by X-ray diffraction (XRD), Scanning and Transmission electron microscopy (SEM). The spectroscopic techniques such as; FT-IR, UV-VIS absorption and Raman spectroscopy were used to investigate optical response of the synthesized nanoparticles. Optical energy band gap was calculated to be 2.57 eV and 2.21 eV corresponding to direct and indirect transitions, respectively. The magnetic properties of the single crystalline nanoparticle were also investigated by Vibrating sample magnetometer (VSM) and found that they are weak ferromagnetic in nature. The values of saturation magnetization, remanent magnetization and coercivity were found to be 0.5925 emu/g and 0.1642 emu/g and 0.1650 Oe, respectively. Copyright © 2012 American Scientific Publishers.