Browsing by Author "Manish Tripathi"

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A therapeutic approach to target mitochondrial dysfunction using molecular docking studies: Screening of natural drugs for oral carcinoma
(Wolters Kluwer Medknow Publications, 2018) Manish Singh; Manish Tripathi; Alok Singh; Chandra Azad; Indrajeet Gambhir; Brijesh Kumar; Suresh Purohit
Background: Mitochondrial dysfunction is the major cause of various types of cancer, leading to death worldwide. The present study investigated the in silico binding potential of natural flavonoids and essential oils with human cyclophilin D (CyPD) protein. CyPD protein is a major molecular marker for apoptosis and has been reported to be elevated in oral carcinoma. Methods: PubChem database was used to check the efficacy of different active phytoconstituents (kaempferol, quercetin, eugenol, oxyresveratrol, tanshinone 2a, catechin, epicatechin, cinnamaldehyde, and emodin). These compounds were used as ligands to check their potential as anticancer agents against the inner mitochondrial membrane protein, CyPD. Docking studies were performed with the help of Discovery Studio 2.5 and Autodock. Emodin was used as a reference inhibitor to compare the results. Results: The binding energy (B.E.) of the reference inhibitor (known/established drug) emodin was observed-28.9 kcal/mol while novel inhibitors (catechin, cinnamaldehyde, epicatechin, eugenol, kaempferol, oxyresveratrol, quercetin, and tanshinone 2a) exhibited a range from-51.51 to-5.89 kcal/mol. Quercetin, kaempferol, and epicatechin (B.E.:-51.51,-34.79, and-30.62 kcal/mol, respectively) showed strong affinity as compared to reference inhibitor (B.E.:-28.9 kcal/mol). Conclusion: Quercetin, kaempferol, and epicatechin can be used as lead inhibitors against targeting CyPD. © 2018 Pharmacognosy Magazine. Published by Wolters Kluwer.
HBsAg Level as Predictor of Liver Fibrosis in HBeAg Positive Patients With Chronic Hepatitis B Virus Infection
(Elsevier B.V., 2015) Sundeep K. Goyal; Ashok K. Jain; Vinod K. Dixit; Suneet K. Shukla; Mohan Kumar; Jayant Ghosh; Arttrika Ranjan; Neha Gupta; Manish Tripathi
Background and aims: Preliminary data suggests lower serum hepatitis B surface antigen level is associated with more severe liver fibrosis in HBeAg positive patients. We evaluated the association of HBsAg level with biochemical, virological, and histological features in asymptomatic patients with chronic HBV infection. Methods: HBsAg levels were measured at baseline in 481 asymptomatic, treatment naive patients with chronic HBV infection. Subjects were followed-up prospectively (median, 12; range, 8-36 months). Phases of HBV infection were defined after regular monitoring of HBV-DNA and transaminases. Liver histology was scored using the METAVIR system. Results: HBeAg positive (n, 126) patients were significantly younger than HBeAg negative (n, 355), median age 26 vs 30 years; P<0.01. HBV genotype could be determined in 350 patients, 240 (68.57%) had genotype D and 100 (28.57%) had genotype A. HBsAg level had modest correlation with serum HBV DNA(r = 0.6 vs 0.4 in eAg positive & negative respectively). HBeAg+ve patients with fibrosis score≥F2 showed significantly lower median serum HBsAg levels and serum HBV DNA levels compared with patients with F0-F1 score (median, range; 4.51, 2.99-6.10 vs 5.06, 4.13-5.89, P<0.01) and (8.39, 3.85-10.60, P<0.01) respectively. Significant inverse correlation of HBsAg level was found with liver fibrosis in eAg positive group (r = -0.76; P<0.001). HBsAg level cut off value 4.7log10IU/ml predicted moderate to advance fibrosis (F≥2) with 92% sensitivity, 85% specificity & 91% negative predictive value. Conclusion: Lower HBsAg level might reflect the status of advanced liver fibrosis in HBeAg positive chronic hepatitis B subjects. © 2015 INASL.
Intrauterine foreign body presenting as a rectal bezoar and causing chronic diarrhea
(2013) Jayant Ghosh; Sundeep Goyal; Sangey Lamtha; Pankaj Kaushik; Manish Tripathi; Sunit Shukla; Vinod Dixit; A.K. Jain
[No abstract available]
Low vacuum annealing of polymer at low temperatures towards direct and scalable growth of graphene
(Elsevier Ltd, 2018) Rajanish N. Tiwari; Manish Tripathi; Masamichi Yoshimura; A. Kumar
A transfer-free method is demonstrated for the synthesis of graphene flakes directly on insulating substrate (SiO2/Si) by carbon decomposition and diffusion through a layer of nickel. The 100 nm nickel catalyst was deposited on the top of SiO2 (300 nm)/Si substrate. The sandwich structure was prepared wherein spin-coated polymer was used as a carbon source for the graphene formation. The carbon molecules decomposed and diffused into the nickel layer during annealing of sandwich structures in low vacuum (∼5 × 10−1 Pa) at 600–750 °C for 10 min. When cooled to room temperature (25 °C), graphene is formed at the interface between Ni and SiO2/Si, the nickel film was removed by chemical etching. The observed graphene on SiO2/Si substrates is confirmed by optical microscopy, Raman spectroscopy and atomic force microscopy. The Raman spectroscopy mapping over a 55 × 55 μm2 area confirmed the good quality of graphene flakes obtained. © 2018 Elsevier Ltd
Prolonged use of tenofovir and entecavir in hepatitis B virus-related cirrhosis
(Indian Society of Gastroenterology, 2015) Sundeep Kumar Goyal; Vinod Kumar Dixit; Suneet Kumar Shukla; Jayant Ghosh; Manas Behera; Manish Tripathi; Neha Gupta; Arttrika Ranjan; Ashok Kumar Jain
Background and Aims: Limited data is available from India on outcome and efficacy of tenofovir and entecavir in hepatitis B virus (HBV)-related cirrhosis when used for prolonged time. We report the long-term efficacy and outcome of these antiviral drugs in patients with chronic HBV infection, with compensated or decompensated cirrhosis. Methods: We retrospectively analyzed laboratory and clinical data of 400 HBV-related cirrhotic patients without access to liver transplantation, who were treated with tenofovir/entecavir therapy, from January 2007 to January 2014. Two hundred and ten (52.5 %) patients had at least one of the components of decompensation at baseline. Two hundred and twenty (55 %) and 180 (45 %) patients were initiated tenofovir and entecavir, respectively. Follow up period was 45 (12–68) months for tenofovir and 36 (11–60) months for entecavir. Results: At the end of 1 year, levels of HBV DNA <20 IU/mL were achieved in 91.8 % and 88.8 % of patients, and alanine aminotransferase normalized in 54.5 % and 55.5 % of patients who received tenofovir and entecavir, respectively. At the last visit, Child-Turcotte-Pugh scores improved among 29.5 % of patients who received tenofovir, 25 % of those who received entecavir, and remained stable in 61.9 % and 65 % patients, respectively, in both groups. The 5-year cumulative rate of liver decompensation, hepatocellular carcinoma, and cirrhosis-related complications were 3.1 %, 1.9 %, and 2.1 % with an annual incidence of 0.8 %, 0.3 %, and 0.5 % per person-year, respectively. Conclusion: Tenofovir and entecavir were effective and potent drugs for prolonged treatment of HBV cirrhosis and improved the overall clinical course. © 2015, Indian Society of Gastroenterology.
Silver Nanoparticles/Gelatin Composite: A New Class of Antibacterial Material
(Wiley-Blackwell, 2017) Ashwani Kumar Singh; Manish Tripathi; Onkar Nath Srivastava; Rajiv Kumar Verma
A simple and fast, one-step, cost effective protocol for silver nanoparticle (AgNP) synthesis has been reported. AgNO3 is reduced using black cardamom (B.C.) extract to produce nanoparticles. Variable size nanoparticles were obtained by choosing the different composition of a reaction mixture comprised of AgNO3 solution and B.C. extract. Nanoparticle formation was monitored by the color change of reaction mixture as well as by UV-Visible spectroscopy. AgNPs has been characterized thoroughly by XRD and TEM analysis for shape and size. A plausible mechanism for reduction of Ag+ has been proposed based on oxidation products of 1,8-cineole, which is one of the major components of B.C. extract. A very important application of these AgNPs has also been shown by preparing AgNP-gelatin composite to check its activity against different strains of bacteria. The results suggest excellent antibacterial activities of the above-mentioned composite films, which can be further developed as antibacterial films for different purposes. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim