Browsing by Author "Manisha Mishra"

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Bioremediation of contaminated soils: An overview
(Springer Singapore, 2017) Manisha Mishra; Devendra Mohan
Bioremediation has attracted attention of scientists and biotechnologists for environmental control and it employs biological agents mainly microorganisms like yeast, fungi, algae, or bacteria to treat contaminated soils or water derived in the process applied for treatment of such sites. Microbial bioremediation for in-situ removal of organic pollutants, heavy toxic metals, radionuclides, etc., can be applied successfully. Specific bioremediation technologies can also be developed based on toxicity of contaminants and the site conditions. As a component of bioremediation, application of plants for removal of contaminants from environment, known as phytoremediation, can also be made and hyperaccumulator species are capable of accumulating toxic metals about 100 times higher than those typically found in common plants. Uses of a number of biosorbents for soil treatment by uptake of ionic species present even up to tracer levels (around 10-7 M) has been demonstrated successfully. Molecular approaches can be effectively applied to enhance bioremediation. This paper presents an overview of various studies carried out on bioremediation of contaminated soils. © Springer Nature Singapore Pte Ltd. 2017. All rights reserved.
Biosorptive behaviour of casein for Zn2+, Hg2+ and Cr3+: Effects of physico-chemical treatments
(1998) Shuddhodan P. Mishra; Diwakar Tiwari; R.S. Dubey; Manisha Mishra
The uptake behaviour of milk protein (casein) for some heavy metal toxic ions [Zn2+, Hg2+ and Cr3+] was investigated by employing the 'radiotracer technique'. The effects of various physico-chemical treatments were investigated in order to explain the sequestering behaviour of this biomass. In a single batch equilibrium experiment a decrease in adsorptive concentration (10-2-10-8 mol L-1) and increase in temperature (293-323 K) and pH (ca 3-10) specifically increased the uptake of metal ions. The uptake followed a first-order rate law and obeyed the Freundlich isotherm. Changes in standard enthalpy and activation energy involved during the biosorption were deduced for these systems. Also, desorption experiments showed that no significant desorption took place.
Biosorptive behaviour of Mango (Mangifera indica) and Neem (Azadirachta indica) bark for Hg2+, Cr3+ and Cd2+ toxic ions from aqueous solutions: A radiotracer study
(Elsevier Sci Ltd, 1999) Diwakar Tiwari; Shuddhodan P. Mishra; Manisha Mishra; R.S. Dubey
Biosorption of Hg2+ and Cr3+ on dead biomass Mango (Mangifera indica) and Neem (Azadirachta indica) bark has been assessed at micro to tracer level concentrations from aqueous solutions employing the 'radiotracer technique'. A high level of uptake of metal ions on these solid surfaces occurs within ca. 4 h of contact time reaching apparent saturation. The increase of sorptive concentration (10-8 to 10-2 mol dm-3), temperature (293-323 K) and pH (ca. 3 to 10) favoured the removal process of these ions; but in the case of Hg2+ on Neem bark, there was seemingly no temperature effect. The uptake process follows first order rate law and obeys the Freundlich adsorption isotherm. Added anions and cations in the bulk solution inhibit to some extent the removal of these toxic ions. Similarly the inhibition in the uptake was also observed when both biomasses were irradiated by neutron and γ-rays prior to being employed as sorbents. No significant sorption of Cd2+ was observed on these dead biomass solid surfaces under various physical-chemical conditions.; Biosorption of Hg2+ and Cr3+ on dead biomass Mango (Mangifera indica) and Neem (Azadirachta indica) bark has been assessed at micro to tracer level concentrations from aqueous solutions employing the `radiotracer technique'. A high level of uptake of metal ions on these solid surfaces occurs within ca. 4 h of contact time reaching apparent saturation. The increase of sorptive concentration (10-8 to 10-2 mol dm-3), temperature (293-323 K) and pH (ca. 3 to 10) favoured the removal process of these ions; but in the case of Hg2+ on Neem bark, there was seemingly no temperature effect. The uptake process follows first order rate law and obeys the Freundlich adsorption isotherm. Added anions and cations in the bulk solution inhibit to some extent the removal of these toxic ions. Similarly the inhibition in the uptake was also observed when both biomasses were irradiated by neutron and γ-rays prior to being employed as sorbents. No significant sorption of Cd2+ was observed on these dead biomass solid surfaces under various physical-chemical conditions.
Biosorptive behaviour of rice hulls for Cs-134 from aqueous solutions: A radiotracer study
(2007) Shuddhodan P. Mishra; Shailesh K. Prasad; Ram S. Dubey; Manisha Mishra; Diwakar Tiwari; Seung-Mok Lee
Removal behaviour of rice hulls was assessed for the removal of Cs-134 from aqueous solutions. Results obtained from batch-type experiments revealed that relatively low uptake of Cs(I) was favoured with increasing the sorptive concentration (from 1×10-8 to 1×10-2 mol L-1), temperature (298-328), and pH (2.40-10.20). The concentration dependence data fitted well for Freundlich adsorption isotherm. Thermodynamic parameters revealed that the uptake process was endothermic and proceeded via 'ion exchange' along with 'surface complexation'. Moreover, the adsorbed species were not to be desorbed into the bulk concentration even at elevated temperatures, i.e., up to 328 K hence forming a stable adsorption phase. Further, the radiation stability of the rice hulls samples was also assessed by exposing it towards 300 mCi neutron source having the neutron flux of ca. 3.85×106 n cm-2 s-1 associated with nominal γ-dose of ca. 1.72 Gy h-1 and indeed it was observed that the rice hulls samples were found to be stable at least for the removal of Cs-134. © 2006 Elsevier Ltd. All rights reserved.
Environmental contaminants and their management using microorganisms
(Elsevier, 2020) Manisha Mishra; Sandeep Kumar Singh; Monika Singh; Vipin Kumar Singh; Hariom Verma; Kumari Rashmi; Ajay Kumar
Nowadays environment faces rise in various contaminants of both organic and inorganic origin that adversely affect the texture productivity of soil, plants, and ultimately the health of human beings. The traditional physicochemical methods of environment management are still a major challenge due to their toxic residuals products and high cost effectiveness. Bioremediation or the remediation using microbes or their products is an emerging and sustainable method of contaminant management. The microbial strains including bacteria, cyanobacteria, and fungi secrete or synthesize various enzymes, metabolites, surfactant and also produce siderophore, EPS, HCN that help in transforming complex or toxic environmental contaminants such as hydrocarbons, petro products, heavy metals, pesticides, xenobiotics into simpler ones or even complete degradations. In this chapter, we summarize the different aspects of bioremediation and methodologies used by microbial strains used during the bioremediation of various environmental contaminants. © 2021 Elsevier Inc.
Environmental factors affecting the bioremediation potential of microbes
(Elsevier, 2020) Manisha Mishra; Sandeep Kumar Singh; Ajay Kumar
Removal of pollutants in a sustainable manner can be made possible through bioremediation. The process can be done be brought about by biological organisms such as bacteria, fungi, and actinomycetes. The bioremediation process is also important in making free or remediate the waste water and solid wastes rich in organic matter pollutant. Needless to mention, the microorganisms in their metabolic processes release certain enzymes, biosurfactants, and microbial products that convert these hazardous pollutants to less toxic ones. The bioremediation process as such also generates solid residue that finds application as organic manure. However, there are certain limiting factors that need to explore for the effective, sustainable bioremediation process. In the light of these, the present chapter discusses methodology as well as limiting factors of effective, economic, and ecofriendly bioremediation process. © 2021 Elsevier Inc.
Expression of an insecticidal fern protein in cotton protects against whitefly
(Nature Publishing Group, 2016) Anoop Kumar Shukla; Santosh Kumar Upadhyay; Manisha Mishra; Sharad Saurabh; Rahul Singh; Harpal Singh; Nidhi Thakur; Preeti Rai; Paras Pandey; Aradhana L. Hans; Subhi Srivastava; Vikram Rajapure; Sunil Kumar Yadav; Mithlesh Kumar Singh; Jitendra Kumar; K. Chandrashekar; Praveen C. Verma; Ajit Pratap Singh; K.N. Nair; Smrati Bhadauria; Muhammad Wahajuddin; Sarika Singh; Sharad Sharma; Omkar; Ram Sanmukh Upadhyay; Shirish A. Ranade; Rakesh Tuli; Pradhyumna Kumar Singh
Whitefly (Bemisia tabaci) damages field crops by sucking sap and transmitting viral diseases. None of the insecticidal proteins used in genetically modified (GM) crop plants to date are effective against whitefly. We report the identification of a protein (Tma12) from an edible fern, Tectaria macrodonta (Fee) C. Chr., that is insecticidal to whitefly (median lethal concentration = 1.49 μg/ml in in vitro feeding assays) and interferes with its life cycle at sublethal doses. Transgenic cotton lines that express Tma12 at ∼0.01% of total soluble leaf protein were resistant to whitefly infestation in contained field trials, with no detectable yield penalty. The transgenic cotton lines were also protected from whitefly-borne cotton leaf curl viral disease. Rats fed Tma12 showed no detectable histological or biochemical changes, and this, together with the predicted absence of allergenic domains in Tma12, indicates that Tma12 might be well suited for deployment in GM crops to control whitefly and the viruses it carries.
Inorganic ion-exchangers in radioactive waste management. Part XVI: Uptake of some metal phosphates (stannic and zirconium) for 134Cs
(2006) Shuddhodan P. Mishra; Diwakar Tiwari; Shailesh Kumar Prasad; R.S. Dubey; Manisha Mishra
The uptake of indigenously synthesized amorphous stannic and zirconium phosphate was assessed for, one of the important fission fragment, cesium from aqueous solutions using a radiotracer technique. A virtual increase in sorptive concentration (from 1.0·10-8 to 1.0·10-2 mol·dm-3) and pH (from 2.4 to 10.2) and temperature (from 303 to 333 K) enhanced the uptake of cesium on stannic phosphate. However, the extremely high degree of uptake of cesium on zirconium phosphate was almost unaffected with the dilution beyond 10-5 mol·dm-3 and pH (i.e., from 2.4 to 10.2) and temperature (from 303 to 323 K). Irreversible uptake occurring for these solids follow the Freundlich adsorption isotherm and the presence of several complexing agents viz., sulphate, phosphate, glycine and EDTA did not affect appreciably the uptake of cesium on zirconium phosphate but it did affect for stannic phosphate system. Both these solids showed good radiation stability towards a 11.1 GBq Ra-Be neutron source having neutron flux ca. 3.2·106 n·cm -2·s-1 and associated with a nominal γ-dose of ca. 1.72 Gy/h, at least for the uptake of cesium. © 2006 Akadémiai Kiadó.
Inorganic particulates in removal of toxic heavy metal ions: Part X: Removal behavior of aluminum hydroxide for Hg(II): A radiotracer study
(2007) S.P. Mishra; D. Tiwari; S.K. Prasad; R.S. Dubey; Manisha Mishra
The removal behavior of amorphous aluminum hydroxide for Hg(II) ions from aqueous solutions was investigated by employing a radiotracer technique at micro down to trace level concentrations. The batch type experiments were performed to obtain various physico-chemical parameters, viz., effect of sorptive concentration, temperature and pH. It was observed that the increase in sorptive concentration (from 1.10-8 to 1.10-2 mol·dm -3), temperature (from 303 to 333K) and pH (from 3.4 to 10.3) apparently favored the uptake of Hg(II) by this solid. Similarly, the presence of anions (six fold) viz., oxalate, phosphate, glycine and EDTA also enhanced the uptake behavior of aluminum hydroxide for Hg(II). Whereas, the added cations viz., Na+, K+, Ba2+, Sr2+, Mg 2+, Cd2+ and Fe3+ more or less suppressed the removal behavior of the adsorbent. Further, the adsorption process followed the classical Freundlich adsorption isotherm and deductions of various thermodynamic data revealed that the uptake of Hg(II) on aluminum hydroxide followed the ion-exchange type mechanism and thermodynamically it was found to be endothermic in nature. © 2007 Springer Science+Business Media, Inc.
Minocycline, levodopa and MnTMPyP induced changes in the mitochondrial proteome profile of MPTP and maneb and paraquat mice models of Parkinson's disease
(2013) Anubhuti Dixit; Garima Srivastava; Divya Verma; Manisha Mishra; Pradhyumna Kumar Singh; Om Prakash; Mahendra Pratap Singh
Mitochondrial dysfunction is the foremost perpetrator of the nigrostriatal dopaminergic neurodegeneration leading to Parkinson's disease (PD). However, the roles played by majority of the mitochondrial proteins in PD pathogenesis have not yet been deciphered. The present study investigated the effects of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) and combined maneb and paraquat on the mitochondrial proteome of the nigrostriatal tissues in the presence or absence of minocycline, levodopa and manganese (III) tetrakis (1-methyl-4-pyridyl) porphyrin (MnTMPyP). The differentially expressed proteins were identified and proteome profiles were correlated with the pathological and biochemical anomalies induced by MPTP and maneb and paraquat. MPTP altered the expression of twelve while combined maneb and paraquat altered the expression of fourteen proteins. Minocycline, levodopa and MnTMPyP, respectively, restored the expression of three, seven and eight proteins in MPTP and seven, eight and eight proteins in maneb- and paraquat-treated groups. Although levodopa and MnTMPyP rescued from MPTP- and maneb- and paraquat-mediated increase in the microglial activation and decrease in manganese-superoxide dismutase expression and complex I activity, dopamine content and number of dopaminergic neurons, minocycline defended mainly against maneb- and paraquat-mediated alterations. The results demonstrate that MPTP and combined maneb and paraquat induce mitochondrial dysfunction and microglial activation and alter the expression of a bunch of mitochondrial proteins leading to the nigrostriatal dopaminergic neurodegeneration and minocycline, levodopa or MnTMPyP variably offset scores of such changes. © 2013 Elsevier B.V.
Plant growth-promoting bacteria: Application in bioremediation of salinity and heavy metal-contaminated soils
(Elsevier, 2020) Divya Singh; Sandeep Kumar Singh; Vipin Kumar Singh; Hariom Verma; Manisha Mishra; Kumari Rashmi; Ajay Kumar
Salinity and accumulation of heavy metals in the soil are some prominent abiotic stress that limits yields and productivity of plant and soil. The physiochemical properties of the soil are highly specific and the ultimate requirement for optimum crop yields; however, the presence of any abiotic stress factors disturbs natural texture as well as physicochemical properties of the soil. Currently, to mitigate the challenges of salinity and heavy metals, various physical and chemical methods have been applied, but cost-effectiveness and the adverse impact of residues on the environment open a new door to find a sustainable and safe approach for the mitigation challenge of salinity and heavy metal stress. In this regard, plant growth-promoting bacteria (PGPB) can be a suitable alternative in mitigation of stress challenge by their natural, eco-friendly, and sustainable approach. Besides remediation, utilization of PGPB benefitted the soil as well as crops by maintaining the nutrients status, synthesizing phytohormones, and solubilizing phosphate. © 2021 Elsevier Inc.
Removal Behaviour of Rice (Oryza sativa L) Hulls for Submicro Concentrations of Hg2+ and Cr3+ from Aqueous Solutions: A Radiotracer Study
(R. Oldenbourg Verlag GmbH, 1998) Shuddhodan P. Mishra; Diwakar Tiwari; R.S. Dubey; Manisha Mishra
The uptake behaviour of rice (Oryza sativa L) hulls for the micro to trace levels of Hg2+ and Cr3+ from aqueous solutions has been carried out in batch equilibrium experiments, employing a 'radiotracer technique'. The parametric study reveals that the increase of adsorptive concentration (10-8 to 10-2 mol dm-3), temperature (293 to 323 K) and pH ca. (3 to 10) enhances the removal of these ions and from thermal and from kinetic data it is inferred that the sorption of these metal ions on rice hulls follows first order rate law, obeys Freundlich isotherm and is endothermic in nature. Desorption experiments allow further conclusion that the process is apparently irreversible as no significant desorption could take place in the bulk concentration of the adsorptives.
Role of omics approaches in microbial bioremediation
(Elsevier, 2020) Manisha Mishra; Sandeep Kumar Singh; Ajay Kumar
Bioremediation is an emerging technology that can clean-up contaminated environments in an effective and economically feasible way. However, gaps exist as the growth, and regulatory mechanisms operating in these microorganisms are not completely known. Earlier approaches in bioremediation included treatability via microbial culture but it has several drawbacks, which can be overcome by metagenomics approaches via studying and exploring uncultured microorganisms present in the environment at the contaminated site. Recent advances in omics technologies, however, are making it possible to understand microbial physiology and their metabolic functioning as well. Availability of genome sequences of most of the microorganisms involved in bioremediation and advancement in the omics approaches including transcriptomics, proteomics and metabolomics have led to much progress in the understanding of metabolic functioning at the level of microbial cell to that of the entire microbial population. It is expected that the omics tools will further help in understanding the response of microbial communities to the expanding list of contaminants and xenobiotics that would lead to successful bioremediation initiatives worldwide. The present chapter is a small effort to discuss the omics approaches in bioremediation. © 2021 Elsevier Inc.