Browsing by Author "Manmath Kumar Nandi"

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Targeting biosignatures of hyperglycemia and oxidative stress in diabetes comorbid depressive rats: effectiveness of hydroethanolic extract of the whole plant of Ludwigia octovalvis
(TMR Publishing Group, 2023) Stuti Pandey; Pawan Kumar Pandey; Alakh Niranjan Sahu; Himanshu Verma; Manmath Kumar Nandi
Background: The prime objective of the current research was to evaluate the whole plant hydroalcoholic extract of Ludwigia octovalvis (HLO) against hyperglycemia, and oxidative stress biomarkers in rats induced with diabetes comorbid depression, diabetes comorbid depression (streptozotocin-nicotinamide + electric footshocks). Methods: 2,2-Diphenyl-1-picrylhydrazyl assay of HLO versus ascorbic acid was done. Effects of 200 and 400 mg/kg body weight/day HLO doses versus 25 mg/kg body weight/day metformin was studied through insulin, glucose, superoxide dismutase, lipid peroxidation, catalase, and behavioral assessment (forced swim and open field tests). Results: IC50 values of HLO and ascorbic acid were 33.52 and 27.86 µg/mL respectively. Both the HLO doses showed intended results with respect to oxidative stress biomarkers in diabetes comorbid depression rats in comparison to metformin. Open field test showed better results for HLO in diabetes comorbid depression rats. However, hypoglycemic effects, and forced swim test performance of metformin was slightly higher than the 400 mg dose, followed by the 200 mg dose of HLO. Ethyl gallate, gallic acid, β-sitosterol, and quercetin in HLO might resulted in attenuating diabetic as well as depression biomarkers. Conclusion: Inhibition of glucosidase and lipase activity, and AMP-activated protein kinase phosphorylation might be the possible biochemical changes occurred in HLO treated rats. © 2023 By Author(s). Published by TMR Publishing Group Limited.
Toxicity (acute and subacute) assessment and in-vivo antiurolithiatic activity of ethanolic extract of Caesalpinia bonducella seed in albino Wistar rat
(Open Science Publishers LLP Inc., 2022) Ajay Kumar; Manmath Kumar Nandi; Brijesh Kumar; Ashutosh Kumar; Rajesh Kumar; Vikas Kailashiya; Ambrish Kumar Singh
The objective of the present work is to find supportive evidence for the use of Caesalpinia bonducella in nephrolithiasis. For safety assessment of ethanolic extract of seed of C. bonducella (ECB), acute and subacute oral toxicity studies was performed. In the acute oral toxicity study at a dose of 2,000 mg/kg and subacute oral toxicity study at dose of 250, 500, and 1,000 mg/kg, no changes were seen in the behavior, haematological parameters, biochemical parameters, histopathology of major organ, and body weight of rats. The efficacy of ECB at dose of 200 and 400 mg/kg was studied in ethylene glycol, glycolic acid, and sodium oxalate induced urolithiasis in rats and marketed cystone tablet at a dose of 750 mg/kg was used as the standard drug. The mean body weight difference, urinary parameters (urine pH, urine volume, levels of calcium, magnesium, and phosphorus), and serum biochemical parameters [urea, Blood urea nitrogen (BUN), creatinine, uric acid, and alkaline phosphatase] were measured. Urolithiasis caused a significant decrease in mean body weight, pH, and volume of urine and increased in both serum biochemical parameters and urinary electrolyte concentration as compared with the normal control group. Histopathology of kidneys were showed increased glomerular hypercellularity, hydropic tubular degeneration, and cast in the induced control group. Ethanolic Extract of Caesalpinia bonducella (ECB) was found to be effective to normalise the above all changes. It was concluded that the ECB was effective in the prevention and elimination of calcium oxalate crystal deposition into kidneys. © 2022. Ajay Kumar et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).