Browsing by Author "Martin Valis"

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Anastrozole-mediated modulation of mitochondrial activity by inhibition of mitochondrial permeability transition pore opening: an initial perspective
(Taylor and Francis Ltd., 2023) Somesh Kumar; Neha Choudhary; Mohammed Faruq; Arun Kumar; Ravindra K. Saran; Prem Kumar Indercanti; Vikram Singh; Haseena Sait; Sunita Jaitley; Martin Valis; Kamil Kuca; Sunil K. Polipalli; Manoj Kumar; Tejveer Singh; Prashanth Suravajhala; Rohit Sharma; Seema Kapoor
The mitochondrial permeability transition pore (mtPTP) plays a vital role in altering the structure and function of mitochondria. Cyclophilin D (CypD) is a mitochondrial protein that regulates mtPTP function and a known drug target for therapeutic studies involving mitochondria. While the effect of aromatase inhibition on the mtPTP has been studied previously, the effect of anastrozole on the mtPTP has not been completely elucidated. The role of anastrozole in modulating the mtPTP was evaluated by docking, molecular dynamics and network-guided studies using human CypD data. The peripheral blood mononuclear cells (PBMCs) of patients with mitochondrial disorders and healthy controls were treated with anastrozole and evaluated for mitochondrial permeability transition pore (mtPTP) function and apoptosis using a flow cytometer. Spectrophotometry was employed for estimating total ATP levels. The anastrozole–CypD complex is more stable than cyclosporin A (CsA)–CypD. Anastrozole performed better than cyclosporine in inhibiting mtPTP. Additional effects included inducing mitochondrial membrane depolarization and a reduction in mitochondrial swelling and superoxide generation, intrinsic caspase-3 activity and cellular apoptosis, along with an increase in ATP levels. Anastrozole may serve as a potential therapeutic agent for mitochondrial disorders and ameliorate the clinical phenotype by regulating the activity of mtPTP. However, further studies are required to substantiate our preliminary findings. Communicated by Ramaswamy H. Sarma. © 2023 Informa UK Limited, trading as Taylor & Francis Group.
Anti-viral drug discovery against monkeypox and smallpox infection by natural curcumin derivatives: A Computational drug design approach
(Frontiers Media S.A., 2023) Shopnil Akash; Arafat Hossain; Md. Sarowar Hossain; Md. Mominur Rahman; Mohammad Z. Ahmed; Nemat Ali; Martin Valis; Kamil Kuca; Rohit Sharma
Background: In the last couple of years, viral infections have been leading the globe, considered one of the most widespread and extremely damaging health problems and one of the leading causes of mortality in the modern period. Although several viral infections are discovered, such as SARS CoV-2, Langya Henipavirus, there have only been a limited number of discoveries of possible antiviral drug, and vaccine that have even received authorization for the protection of human health. Recently, another virial infection is infecting worl dwi de (Monkeypox, and Smal l pox), whi ch concerns pharmaci sts, biochemists, doctors, and healthcare providers about another epidemic. Also, currently no specific treatment is available against Monkeypox. This research gap encouraged us to develop a new molecule to fight against monkeypox and smallpox disease. So, firstly, fifty different curcumin derivatives were collected from natural sources, which are available in the PubChem database, to determine antiviral capabilities against Monkeypox and Smallpox. Material and method: Preliminarily, the molecular docking experiment of fifty different curcumin derivatives were conducted, and the majority of the substances produced the expected binding affinities. Then, twelve curcumin derivatives were picked up for further analysis based on the maximum docking score. After that, the density functional theory (DFT) was used to determine chemical characterizations such as the highest occupied molecular orbital (HOMO), lowest unoccupied molecular orbital (LUMO), softness, and hardness, etc. Results: The mentioned derivatives demonstrated docking scores greater than 6.80 kcal/mol, and the most significant binding affinity was at-8.90 kcal/mol, even though 12 molecules had higher binding scores (-8.00 kcal/mol to-8.9 kcal/mol), and better than the standard medications. The molecular dynamic simulation is described by root mean square deviation (RMSD) and root-mean-square fluctuation (RMSF), demonstrating that all the compounds might be stable in the physiological system. Conclusion: In conclusion, each derivative of curcumin has outstanding absorpti on, di stri buti on, metabol i sm, excretion, and toxi ci ty (ADMET) characteristics. Hence, we recommended the aforementioned curcumin derivatives as potential antiviral agents for the treatment of Monkeypox and Smallpox virus, and more in vivo investigations are warranted to substantiate our findings. © 2023 Akash, Hossain, Hossain, Rahman, Ahmed, Ali, Valis, Kuca and Sharma.
Bioinspired metal/metal oxide nanoparticles: A road map to potential applications
(Elsevier Ltd, 2022) Prashant B. Chouke; Trupti Shrirame; Ajay K. Potbhare; Aniruddha Mondal; Ankita R. Chaudhary; Sudip Mondal; Sanjay R. Thakare; Eugenie Nepovimova; Martin Valis; Kamil Kuca; Rohit Sharma; Ratiram Gomaji Chaudhary
Manufacturing of metal and metal oxide nanoparticles (M/MO NPs) in large quantities needed a strong reliable, sustainable, and eco-friendly protocol. Present work represents on biogenic approaches to fabricate green nanoparticles using green technology. The fabrications of M/MO NPs using natural bio-resources were engaged by means of alternative technique in place of conventional methods. These methods are naturally benign, straightforward, economical, and renewed technology; they does not content harmful chemicals, zero contaminants, and eco-friendly. The extracts from the biogenic resources are widely accepted owing to its capability to minimise and control the size and shape of metal and metal oxides NPs because of different structure directing agents, usually bioorganic phyto-chemicals. In this present review, we have summarized fabrication of different NPs like silver, gold, copper oxide, cobalt oxide, titanium oxide, cerium oxide, bismuth oxide, zinc oxide and nickel oxide nanoparticles using natural resources. The challenges, limiting factors and future directions of the bioinspired synthesis of metal/metal oxide NPs are also highlighted in this review. Moreover, biogenic materials has explored for further environmental remediation in terms of photocatalytic activity, elimination of organic waste, and antibacterial, antioxidant assay, and protein-metal complexes binding affinities by molecular docking. © 2022 The Author(s)
Corrigendum: Anti-viral drug discovery against monkeypox and smallpox infection by natural curcumin derivatives: a computational drug design approach(Front. Cell. Infect. Microbiol., (2023), 13, (1157627), 10.3389/fcimb.2023.1157627)
(Frontiers Media S.A., 2023) Shopnil Akash; Arafat Hossain; Md. Sarowar Hossain; Md. Mominur Rahman; Mohammad Z. Ahmed; Nemat Ali; Martin Valis; Kamil Kuca; Rohit Sharma
In the published article, there was an error. The error in our article is in the abstract section. Before 6.8 kcal/mol, a minus (-) sign will be added and it will be -6.8 kcal/mol. A correction has been made to Abstract, Result, Three. “The mentioned derivatives demonstrated docking scores greater than 6.80 kcal/mol, and the most significant binding affinity was at -8.90 kcal/mol, even though 12 molecules had higher binding scores (-8.00 kcal/mol to -8.9 kcal/mol), and better than the standard medications”. The corrected sentence appears below: “The mentioned derivatives demonstrated docking scores greater than -6.80 kcal/mol, and the most significant binding affinity was at -8.90 kcal/mol, even though 12 molecules had higher binding scores (-8.00 kcal/mol to -8.9 kcal/mol), and better than the standard medications”. The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. In the published article, there was an error. The 2nd error in our article is in the literature review section. The sub section 2.7 Effects of Curcumin on herpes simplex virus should be Effects of Curcumin on Hepatitis C virus instead of herpes simplex virus. A correction has been made to Literature review, Effects of Curcumin on herpes simplex virus, 0. “Effects of curcumin on herpes simplex virus”. The corrected sentence appears below: “Effects of Curcumin on Hepatitis C virus instead of herpes simplex virus”. The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. Copyright © 2023 Akash, Hossain, Hossain, Rahman, Ahmed, Ali, Valis, Kuca and Sharma.
Designing of SiO2 mesoporous nanoparticles loaded with mometasone furoate for potential nasal drug delivery: Ex vivo evaluation and determination of pro-inflammatory interferon and interleukin mRNA expression
(Frontiers Media SA, 2023) Yasir Mehmood; Hira Shahid; Kashif Barkat; Muhammad Ibraheem; Humayun Riaz; Syed Faisal Badshah; Hitesh Chopra; Rohit Sharma; Eugenie Nepovimova; Kamil Kuca; Martin Valis; Talha Bin Emran
The main objective of the current research work was to synthesize mesoporous silica nanoparticles for controlled delivery of mometasone furoate for potential nasal delivery. The optimized sol–gel method was used for the synthesis of mesoporous silica nanoparticles. Synthesized nanoparticles were processed through Zeta sizer, SEM, TEM, FTIR, TGA, DSC, XRD, and BET analysis for structural characterization. The in vitro dissolution test was performed for the inclusion compound, while the Franz diffusion experiment was performed for permeability of formulation. For the determination of expression levels of anti-inflammatory cytokines IL-4 and IL-5, RNA extraction, reverse transcription, and polymerase chain reaction (RT-PCR) were performed. The MTT assay was also performed to determine cell viability. Synthesized and functionalized mesoporous silica nanoparticles showed controlled release of drugs. FT-IR spectroscopy confirmed the presence of the corresponding functional groups of drugs within mesoporous silica nanoparticles. Zeta sizer and thermal analysis confirmed the delivery system was in nano size and thermally stable. Moreover, a highly porous system was observed during SEM and TEM evaluation, and further it was confirmed by BET analysis. Greater cellular uptake with improved permeability characteristics was also observed. As compared to the crystalline drug, a significant improvement in the dissolution rate was observed. It was concluded that stable mesoporous silica nanoparticles with significant porosity were synthesized, efficiently delivering the loaded drug without any toxic effect. Copyright © 2023 Mehmood, Shahid, Barkat, Ibraheem, Riaz, Badshah, Chopra, Sharma, Nepovimova, Kuca, Valis and Emran.
Exploring the role of nanomedicines for the therapeutic approach of central nervous system dysfunction: At a glance
(Frontiers Media S.A., 2022) Md. Mominur Rhaman; Md. Rezaul Islam; Shopnil Akash; Mobasharah Mim; Md. Noor alam; Eugenie Nepovimova; Martin Valis; Kamil Kuca; Rohit Sharma
In recent decades, research scientists, molecular biologists, and pharmacologists have placed a strong emphasis on cutting-edge nanostructured materials technologies to increase medicine delivery to the central nervous system (CNS). The application of nanoscience for the treatment of neurodegenerative diseases (NDs) such as Alzheimer’s disease (AD), Parkinson’s disease (PD), multiple sclerosis (MS), Huntington’s disease (HD), brain cancer, and hemorrhage has the potential to transform care. Multiple studies have indicated that nanomaterials can be used to successfully treat CNS disorders in the case of neurodegeneration. Nanomedicine development for the cure of degenerative and inflammatory diseases of the nervous system is critical. Nanoparticles may act as a drug transporter that can precisely target sick brain sub-regions, boosting therapy success. It is important to develop strategies that can penetrate the blood–brain barrier (BBB) and improve the effectiveness of medications. One of the probable tactics is the use of different nanoscale materials. These nano-based pharmaceuticals offer low toxicity, tailored delivery, high stability, and drug loading capacity. They may also increase therapeutic effectiveness. A few examples of the many different kinds and forms of nanomaterials that have been widely employed to treat neurological diseases include quantum dots, dendrimers, metallic nanoparticles, polymeric nanoparticles, carbon nanotubes, liposomes, and micelles. These unique qualities, including sensitivity, selectivity, and ability to traverse the BBB when employed in nano-sized particles, make these nanoparticles useful for imaging studies and treatment of NDs. Multifunctional nanoparticles carrying pharmacological medications serve two purposes: they improve medication distribution while also enabling cell dynamics imaging and pharmacokinetic study. However, because of the potential for wide-ranging clinical implications, safety concerns persist, limiting any potential for translation. The evidence for using nanotechnology to create drug delivery systems that could pass across the BBB and deliver therapeutic chemicals to CNS was examined in this study. Copyright © 2022 Rhaman, Islam, Akash, Mim, Noor alam, Nepovimova, Valis, Kuca and Sharma.
Metformin: Activation of 5′ AMP-activated protein kinase and its emerging potential beyond anti-hyperglycemic action
(Frontiers Media S.A., 2022) Sanjay Goel; Ravinder Singh; Varinder Singh; Harmanjit Singh; Pratima Kumari; Hitesh Chopra; Rohit Sharma; Eugenie Nepovimova; Martin Valis; Kamil Kuca; Talha Bin Emran
Metformin is a plant-based drug belonging to the class of biguanides and is known to treat type-2 diabetes mellitus (T2DM). The drug, combined with controlling blood glucose levels, improves the body’s response to insulin. In addition, trials have identified the cardioprotective potential of metformin in the diabetic population receiving the drug. Activation of 5′ AMP-activated protein kinase (AMPK) is the major pathway for these potential beneficial effects of metformin. Historically, much emphasis has been placed on the potential indications of metformin beyond its anti-diabetic use. This review aims to appraise other potential uses of metformin primarily mediated by the activation of AMPK. We also discuss various mechanisms, other than AMPK activation, by which metformin could produce beneficial effects for different conditions. Databases including PubMed/MEDLINE and Embase were searched for literature relevant to the review’s objective. Reports from both research and review articles were considered. We found that metformin has diverse effects on the human body systems. It has been shown to exert anti-inflammatory, antioxidant, cardioprotective, metabolic, neuroprotective, anti-cancer, and antimicrobial effects and has now even been identified as effective against SARS-CoV-2. Above all, the AMPK pathway has been recognized as responsible for metformin’s efficiency and effectiveness. Owing to its extensive potential, it has the capability to become a part of treatment regimens for diseases apart from T2DM. Copyright © 2022 Goel, Singh, Singh, Singh, Kumari, Chopra, Sharma, Nepovimova, Valis, Kuca and Emran.
The role of microRNA-21 in the onset and progression of cancer
(Future Medicine Ltd., 2021) Ashutosh Singh; Ashutosh Kumar Singh; Rajanish Giri; Dhruv Kumar; Rohit Sharma; Martin Valis; Kamil Kuca; Neha Garg
MicroRNAs (miRNAs), a class of small noncoding RNA, posttranscriptionally regulate the expression of genes. Aberrant expression of miRNA is reported in various types of cancer. Since the first report of oncomiR-21 involvement in the glioma, its upregulation was reported in multiple cancers and was allied with high oncogenic property. In addition to the downregulation of tumor suppressor genes, the miR-21 is also associated with cancer resistance to various chemotherapy. The recent research is appraising miR-21 as a promising cancer target and biomarker for early cancer detection. In this review, we briefly explain the biogenesis and regulation of miR-21 in cancer cells. Additionally, the review features the assorted genes/pathways regulated by the miR-21 in various cancer and cancer stem cells. © 2021 Newlands Press.