Browsing by Author "Mithilesh Kumar Singh"

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Antioxidant supplementation during treatment of outpatient dogs with parvovirus enteritis ameliorates oxidative stress and attenuates intestinal injury: A randomized controlled trial
(Elsevier B.V., 2023) Gollahalli Eregowda Chethan; Ujjwal Kumar De; Mithilesh Kumar Singh; Vishal Chander; Raguvaran Raja; Babul Rudra Paul; Om Prakash Choudhary; Neeraj Thakur; Kalyan Sarma; Hridayesh Prasad
A prospective randomized controlled clinical study was conducted to determine whether antioxidant supplementation as an adjunct therapy alters hemogram, oxidative stress, serum intestinal fatty acid binding protein-2 (IFABP-2) level, fecal viral load, clinical score (CS) and survivability in outpatient canine parvovirus enteritis (CPVE) dogs. The dogs with CPVE were randomized to one of the five treatment groups: supportive treatment (ST) alone, ST with N-acetylcysteine (ST+NAC), resveratrol (ST+RES), coenzyme Q10 (ST+CoQ10) or ascorbic acid (ST+AA). The primary outcome measures were reduction of CS and fecal HA titre, and enhancement of survivability. Secondary outcome measures were reduction of oxidative stress indices and IFABP-2 level from day 0 to day 7. The mean CS and HA titre were significantly (P < 0.05) decreased from day 0 to 7 in ST and all antioxidant groups. The supplementations of NAC, RES and AA along with ST markedly (P < 0.05) reduced the concentrations of malondialdehyde, nitric oxide and IFABP-2 on day 7 as compared to ST alone. Additionally, NAC and RES supplementations markedly (P < 0.05) improved the total leukocyte count and neutrophil count in CPVE-affected dogs. NAC and RES could serve as better antioxidants for the amelioration of oxidative stress in CPVE but, the antioxidants did not confer any additional benefits in reduction of CS, fecal HA tire, or survivability when compared with ST alone. © 2023
Multiomics Approaches with Advanced Breeding Tools for Crop Improvement
(CABI International, 2025) Garima Aggarwal; Shalu Kumari; Namata Kumari; Kajal Mehra; Mayank Tiwari; Mithilesh Kumar Singh; Bishawajit Kumar
The escalating challenges posed by climate change, environmental stresses, and rapid population growth call for urgent advancements in crop strategies to ensure global food security. Central to this mission is the development of resilient crop varieties through higher genetic gains, achieved by leveraging modern molecular breeding techniques such as marker-assisted and genomic selection. Multiomics approaches encompassing genomics, transcriptomics, proteomics, and metabolomics offer valuable insights into the intricate biological mechanisms governing crop traits and their interactions with environmental stressors. High-throughput sequencing technologies further enable the identification of essential genes and metabolic pathways, facilitating the development of high-yielding, stress-tolerant varieties. Tools like genome-wide association studies and quantitative trait loci mapping have refined gene discovery, accelerating crop improvement. In combination with genome editing technologies like CRISPR/Cas9, multiomics empowers breeders to create climate-resilient and nutrient-enhanced crops. This chapter delves into the transformative potential of multiomics in plant breeding, emphasizing its role in linking genotype and phenotype through systems biology. Additionally, it addresses challenges and future directions for integrating these advanced methods into agricultural practices, ensuring sustainable solutions in a rapidly changing global environment. The collaboration between multiomics and modern breeding technologies underscores the importance of interdisciplinary research in tackling the pressing demands of food security. © CAB International 2025. All rights reserved.
Omics Approaches for Climate-resilient Oilseed Crops
(CABI International, 2025) Namata Kumari; Bishawajit Kumar; Mithilesh Kumar Singh; Kajal Mehra; Mayank Tiwari; Shalu Kumari; Garima Aggarwal
The increasing challenges posed by climate change, including rising global temperatures, erratic rainfall patterns, and intensifying abiotic and biotic stresses, threaten the productivity of oilseed crops critical to global food, nutritional, and energy security. Abiotic stresses such as drought, heat, salinity, and cold severely impair physiological processes like photosynthesis, nutrient uptake, and water-use efficiency, reducing yield and quality. Biotic stresses, exacerbated by climate change, further compound these issues by promoting pests, diseases, and weed proliferation. Developing climate-resilient oilseed crops through advanced breeding strategies and omics technologies presents a promising solution. Omics approaches—genomics, transcriptomics, proteomics, metabolomics, phenomics, and ionomics—enable the identification and manipulation of stress-responsive genes, proteins, and metabolites, enhancing crop resilience. Integrated omics-assisted breeding, combined with the exploration of genetic diversity from crop wild relatives and underutilized species, offers a sustainable path to improving oilseed crop adaptation. This chapter delves into the potential of multiomics technologies to address abiotic and biotic stress in oilseed crops, fostering the development of climate-resilient cultivars and ensuring sustainable oil production amid global climate change. © CAB International 2025. All rights reserved.