Browsing by Author "Moin Akhtar"

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Expression analysis of droughtinduced genes in wild tomato line (Solanum habrochaites)
(Indian Academy of Sciences, 2014) Ranjit Singh Gujjar; Moin Akhtar; Ashutosh Rai; Major Singh
Many plant genes are regulated in response to abiotic stresses such as drought, high salinity, heat and cold, and their gene products function in stress response and tolerance. The whole process of plant adaptation to these environmental stresses is controlled by orchestration of complex molecular networks. In the present study, eight genes showing significant difference of expression on exposure to artificial drought stress in tomato, were selected from the previously performed microarray experiment. Expression analysis of the genes was done semi-quantitatively as well as quantitatively under artificially imposed drought stress and the results were almost similar to those of microarray experiment. Tissue-specific analysis of the genes, performed on tolerant line, revealed fairly a similar pattern of expression in root, stem and leaf with notable differences in flower, which experienced the least influence of drought. The results confirmed that SlPRP16, SlCYP51-17, SlMCPI19 and SlGDSL20 were downregulated in both the lines with stronger downregulation in sensitive line. SlWRKY4 was downregulated in both the lines with more folds of downregulation in tolerant line. SlEFH12 and SlSNF4-15 were upregulated in tolerant line. SlUSPA9 was upregulated in both the lines with relatively more folds of upregulation in sensitive line.
Pyramiding of genes for grain protein content, grain quality, and rust resistance in eleven Indian bread wheat cultivars: a multi-institutional effort
(Springer Science and Business Media B.V., 2022) Pushpendra K. Gupta; Harindra S. Balyan; Parveen Chhuneja; Jai P. Jaiswal; Shubhada Tamhankar; Vinod K. Mishra; Navtej S. Bains; Ramesh Chand; Arun K. Joshi; Satinder Kaur; Harinderjeet Kaur; Gurvinder S. Mavi; Manoj Oak; Achla Sharma; Puja Srivastava; Virinder S. Sohu; Pramod Prasad; Priyanka Agarwal; Moin Akhtar; Saurabh Badoni; Reeku Chaudhary; Vijay Gahlaut; Rishi Pal Gangwar; Tinku Gautam; Vandana Jaiswal; Ravi Shekhar Kumar; Sachin Kumar; M. Shamshad; Anupama Singh; Sandhya Taygi; Neeraj Kumar Vasistha; Manish Kumar Vishwakarma
Improvement of grain protein content (GPC), loaf volume, and resistance to rusts was achieved in 11 Indian wheat cultivars that are widely grown in four different agro-climatic zones of India. This involved use of marker-assisted backcross breeding (MABB) for introgression and pyramiding of the following genes: (i) the high GPC gene Gpc-B1; (ii) HMW glutenin subunits 5 + 10 at Glu-D1 loci, and (iii) rust resistance genes, Yr36, Yr15, Lr24, and Sr24. GPC increased by 0.8 to 3.3%, although high GPC was generally associated with yield penalty. Further selection among high GPC lines allowed identification of progenies with higher GPC associated with improvement in 1000-grain weight and grain yield in the backgrounds of the following four cultivars: NI5439, UP2338, UP2382, and HUW468. The high GPC progenies (derived from NI5439) were also improved for grain quality using HMW glutenin subunits 5 + 10 at Glu-D1 loci. Similarly, progenies combining high GPC and rust resistance were obtained in the backgrounds of following five cultivars: Lok1, HD2967, PBW550, PBW621, and DBW1. The improved pre-bred lines developed following multi-institutional effort should prove a valuable source for the development of cultivars with improved nutritional quality and rust resistance in the ongoing wheat breeding programmes. © 2022, The Author(s), under exclusive licence to Springer Nature B.V.