Browsing by Author "Mumtaz Ahmad Ansari"

Now showing 1 - 16 of 16

A massive air-fluid level on abdominal skiagram: an indicator of hollow viscous perforation inside an abdominal cocoon
(Springer, 2022) Vivek Srivastava; Pratik K. Jha; Mohit Mangla; Mumtaz Ahmad Ansari
Abdominal cocoon syndrome or sclerosing encapsulating peritonitis is a rare cause of intestinal obstruction due to tuberculosis. It may be complicated further with bowel obstruction and perforation. Perforation of hollow viscera inside a cocoon may present with features of peritonitis. Treatment is by excision of the dense sclerosing membrane over the bowel loops addressing the complications if present and specific medical therapy as indicated. However, there is a high risk of recurrence. We hereby describe a case of a 28 years gentleman who presented in emergency department with acute abdomen along with the clinical features of peritonitis and a single large air-fluid level on erect abdominal skiagram. © 2021, Association of Surgeons of India.
Anti-cancer potential of non-curcuminoid bioactive from Curcuma caesia Roxb. (Black Turmeric): Targeting cervical cancer via PI3K/Akt pathway modulation
(Elsevier Masson s.r.l., 2025) Bishnu Prasad Parida; Megha Periyappilly Radhakrishnan; Varsha Goyal; Astha Sharma; Rupesh Zarekar; Mumtaz Ahmad Ansari; Jasmeet Singh; Sunita K. Singh; Gopeshwar Narayan
Curcuma caesia, or black turmeric, is a significant medicinal plant used in traditional Indian medicine, including Ayurveda and folk practices. Indigenous communities in the northern Indo-Gangetic plains have utilized it for its analgesic, anti-inflammatory, and anti-infective properties. However, the anti-cancer potential of non-curcuminoid bioactive from Curcuma caesia remains underexplored. The current study investigates the anti-cancer potential of non-curcuminoid bioactive compounds derived from Curcuma caesia rhizome extracts, focusing on their effects against cervical cancer. Using high-resolution mass spectrometry (HRMS), key compounds were identified from hexane (HECC) and methanolic (MECC) extracts, among which 3,4-dihydrocoumarin and (+)-ar-turmerone were prominent. In vitro cytotoxicity assays demonstrated that both HECC and MECC selectively inhibited the viability of cervical cancer cell lines, sparing non-tumorigenic HEK293T cells. Mechanistic analyses revealed that 3,4-dihydrocoumarin treatment led to mitochondrial membrane hyperpolarization, suppression of intracellular ROS, and cell cycle arrest at subG1 and G1 phases, while (+)-ar-turmerone had antagonistic modulatory effects. Western blotting confirmed downregulation of PI3K and Akt protein expression. Complementary ADME profiling indicated favorable pharmacokinetic properties, while molecular docking supported strong binding affinity of 3,4-dihydrocoumarin to PI3K and Akt targets, reinforcing its mechanism of action. Curcuma caesia rhizome fractions, especially 3,4-dihydrocoumarin, exhibit anti-cancer properties by modulating key molecular pathways, including the PI3K/Akt pathway. © The Authors
Burden of Chronic Nonhealing Wounds: An Overview of the Worldwide Humanistic and Economic Burden to the Healthcare System
(SAGE Publications Inc., 2024) Aditya Sharma; Ravi Shankar; Ashish Kumar Yadav; Arvind Pratap; Mumtaz Ahmad Ansari; Vivek Srivastava
Chronic wounds have long been a significant public health concern, but the true impact of these wounds is unknown since research designs and measuring techniques vary, leading to inconsistent estimates. The definition of a wound is a loss of epithelial continuity caused by damage to the tissue. The following conditions can cause chronic wounds: panniculitis, pyoderma gangrenosum, traumatic, neurological, metabolic, hematologic, neoplastic, or infection-related. The growing global incidence of diabetes and the aging population necessitate greater attention to chronic wounds. Regrettably, it is sad that significant healthcare institutions have overlooked wound research. The study of health-related illnesses and occurrences in particular populations, including their distribution, frequency, and determinants, and the application of this research to control health problems. © The Author(s) 2024.
Cholecysto-hydatid cyst fistula
(2004) Anand Kumar; Divya Narain Upadhyaya; Seema Singh; Mohan Kumar; Mumtaz Ahmad Ansari
A 27-year-old woman developed recurrent hydatid of liver. CT scan showed unilocular cysts in segments IV and VII. Intraoperatively, there was a fistulous communication between the gall bladder and the cyst in segment IV. Partial pericystectomy along with cholecystectomy was done for the segment IV cyst; percutaneous aspiration, instillation and re-aspiration using hypertonic saline was done for the cyst in segment VII. This was followed by albendazole treatment.
Clinical Significance of Frequently Down-Regulated Phosphatidylethanolamine-Binding Protein-1 in Gallbladder Cancer
(Springer, 2024) Amisha Bharti; Mumtaz Ahmad Ansari; Mallika Tewari; Gopeshwar Narayan; Sunita Singh
Background: Promoter hypermethylation of tumor suppressor genes has been demonstrated to be one of the major mechanisms of their epigenetic regulation in various reports. We have studied the promoter methylation status of PEBP1 and evaluated its correlation with gallbladder carcinogenesis. Aims: PEBP1, an endogenous inhibitor of Raf/MEK/ERK signaling pathway, is a tumor suppressor gene. We aimed to study the expression profile of PEBP1 and understand the mechanism and significance of its deregulation in gallbladder cancer. Methods: PEBP1 expression analysis and its promoter methylation status were investigated in 77 gallbladder carcinoma (GBC) and tissue biopsies from 28 patients of gallstone disease by RT-PCR and MS-PCR, respectively. Results: Our results of the mRNA expression profiling demonstrate that PEBP1 is down-regulated in 62.3% (48/77), while 31.2% (24/77) of the gallbladder cancer biopsies show no significant change and 6.5% (5/77) show up-regulated expression compared to tissue samples of gallstone diseases. In GBC, 48.1% (N = 37) GBC biopsy samples exhibited significantly heterozygous promoter hypermethylation compared to tissue samples from gallstone diseases which show promoter hypermethylation in 3 (10.7%) samples only. In gallbladder cancer, the PEBP1 methylation is significantly associated with lymph node metastasis and shorter period of survival. Conclusion: PEBP1 is frequently down-regulated and hypermethylated in gallbladder cancer and its promoter hypermethylation is a frequent and early inactivating mechanism in GBC. © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023.
Clinical Significance of Overexpression of Oct4 in Advanced Stage Gallbladder Carcinoma
(Springer, 2023) Deepika Singh; Dipanjan Biswas; Mallika Tewari; Amrita Ghosh Kar; Mumtaz Ahmad Ansari; Sunita Singh; Gopeshwar Narayan
Background: Oct4 has critical role in maintaining pluripotency, proliferative potential, and self-renewal capacity in embryonic stem and germ cells. Although Oct4 has been shown to be upregulated in many cancers, its clinical significance in gallbladder carcinoma is poorly understood. Methods: We studied the expression profile of Oct4 in 61 GBC and 30 chronic cholecystitis (as control) using real time RT-PCR, western blotting, and immunohistochemistry. The expression data was correlated with clinico-pathological parameters. The diagnostic utility was assessed through ROC curve, and prognostic value was analyzed by Kaplan–Meier method. Results: Oct4 was significantly upregulated at mRNA as well as protein levels. The higher mRNA expression shows significant association with late stage, late T stage, and higher grade of tumor. A significant positive correlation was also observed with stage, T stage, and tumor grade. Sum score analysis of protein expression shows positive correlation with stage and the presence or absence of gallstone in tumor samples. The ROC curve analysis revealed the moderate diagnostic potential of Oct4. Kaplan–Meier analysis showed that patients having higher expression of Oct4 were having low mean survival compared with the patients with lower Oct4 expression. Conclusion: In conclusion, our data suggests that higher expression of Oct4 may serve as potential biological indicator for tumor aggressiveness and poor prognosis of GBC. © 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Comparison of Platelet Functions, Plasma Proteins and Lipids in Filarial and Non-Filarial Edema Patients
(John Wiley and Sons Inc, 2025) Sushil Surtani; Ashutosh Kumar; Alka Singh; Mumtaz Ahmad Ansari; Jyotsna Kailashiya
Lymphatic filariasis results in chronic edema, pain, elephantiasis and disfigurement in humans. It was previously reported that platelet aggregation is inhibited in lymphatic filariasis patients, compared to healthy controls. However, it was not clear whether the inhibition was due to filarial parasite infection or due to the presence of edema. This study was planned to compare platelet functions, plasma proteins and lipids in filarial and non-filarial edema patients. Edema patients were tested for the presence of filarial antigens and antibodies in their blood and were grouped as filarial and non-filarial edema patients accordingly. Platelet aggregation, size distribution, platelet activation markers, plasma proteins and lipids were measured in collected blood samples. Results showed that platelet aggregation was significantly inhibited in filarial edema patients, compared to non-filarial edema patients. Soluble P-selectin and beta thromboglobulin showed significant positive correlation with each other only in non-filarial edema patients. Plasma total cholesterol was lower in filarial edema patients, and HDL was lower in only female filarial edema patients. Observations confirm that inhibition of platelet functions is due to filarial parasite infection, not merely due to the presence of edema. Results also indicate uncoupling and disturbances of platelet activation processes. © 2025 John Wiley & Sons Ltd.
Frequent promoter hypermethylation and down regulation of BNIP3: An early event during gallbladder cancer progression
(Elsevier B.V., 2022) Amisha Bharti; Amrita Ghosh Kar; Deepika Singh; Mumtaz Ahmad Ansari; Mallika Tewari; Gopeshwar Narayan; Sunita Singh
Background: Epigenetic alterations have been reported as one of the risk factors of gallbladder cancer. Promoter hypermethylation is associated with high incidence and poor prognosis of GBC. Bcl-2/adenovirus E1B 19 kDa interacting protein 3 is a pro-apoptotic protein member of Bcl-2 family. Aims: Present study was aimed to investigate expression profile and promoter methylation status of BNIP3 in GBC and its correlation with clinico-pathological parameters. Methods: The expression analysis and methylation status of BNIP3 was performed by semi-quantitative reverse transcription polymerase chain reaction and Methylation-specific polymerase chain reaction respectively in 84 GBC patients and 29 gallstone tissues (used as normal controls). Results: We demonstrate down regulation of BNIP3 in 56% of the GBC samples. BNIP3 promoter is also frequently hypermethylated (69%) in GBC samples. Interestingly, we found that 69% (40/58) of the BNIP3 promoter hypermethylated samples had also reduced expression of BNIP3. Our data demonstrate significant correlation of the mRNA expression and promoter hypermethylation with late stage and nodal metastasis. Hypermethylation of BNIP3 promoter is associated with low overall survival period. Conclusion: Our results suggest that promoter hypermethylation is an early event and can be a frequent mechanism for downregulation of BNIP3 in GBC. © 2022
Noninvasive biomarkers in head and neck squamous cell carcinoma
(CRC Press, 2014) Anand Kumar; Mumtaz Ahmad Ansari; Vivek Srivastava
The 5-year survival rates of head and neck squamous cell carcinomas (HNSCCs) have remained unchanged despite improved locoregional control and reduced treatment-related morbidity. This can be attributed to multiple factors like lack of suitable markers for screening, presentation of the disease at an advanced stage, failure of advanced lesions to respond to treatment, and variation in site-specific behavior of the tumor. The treatment of these cancers is most effective when the tumor burden is lowest and lymphatic spread is the least. The effective therapy for these tumors will depend on early diagnosis and intervention. Unfortunately, no strategy has yet proven to be consistent and effective with reference to its detection at an early stage. However, newer ongoing researches to detect various “biomarkers” that are biological factors within a tumor that affects the molecular process in tumor progression are in vogue. The present biomarkers for HNSCC include the p53 gene and its protein; microsatellite regions throughout the genome; human papillomavirus; proteins and its metabolites involved in cellular proliferation, apoptosis, angiogenesis, genetic information, and their expression as DNA and RNA; intracellular adhesion molecules; epithelial growth factor receptor; and various measures of immune response to cancer. Biomarkers have potential clinical applications not only in early detection of primary disease but they are also helpful in detection of recurrent cancer as well as could be exploited in selecting molecular targets for possible diagnosis and therapy. However, these tests need validation before these can be used in clinical practice. © 2014 by Taylor & Francis Group, LLC.
Noninvasive early markers in gallbladder cancer
(CRC Press, 2014) Mumtaz Ahmad Ansari; Ruhi Dixit; Vijay Kumar Shukla
Gallbladder cancer (GBC) is the most common biliary tree cancer in the world, but it is only 0.5% of all gastrointestinal cancers with lethal malignancy and marked ethnic and geographical variations. The presenting symptoms are typically vague so patient presents in advanced stage. The overall mean survival rate for patients with advanced GBC is 6 months, with a 5-year survival rate of 5% (Levy et al. 2001). Early GBC (confined to the mucosa), though infrequent, offers the potential for a cure by cholecystectomy. Most (>80%) GBCs are adenocarcinomas that originate from the fundus (60%), body (30%), or neck (10%). The basis likely is genetic susceptibility, perhaps elicited by chronic gallbladder inflammation, often a product of cholelithiasis (Pandey 2003). One reasonable hypothesis focuses on chronic irritation of the mucosa (e.g., from the physical presence of the stones and/or superimposed chronic infection such as from Salmonella typhi) leading to dysplasia (perhaps abetted by mutagenic secondary bile acids) and terminating in malignant change. © 2014 by Taylor & Francis Group, LLC.
Pain in acute and chronic wounds: a descriptive study.
(2005) Dinesh Shukla; Anuj Kumar Tripathi; Saurabh Agrawal; Mumtaz Ahmad Ansari; Amit Rastogi; Vijay Kumar Shukla
The significance of pain in wound healing is much neglected because of biases against pain management in general, a lack of knowledge of available analgesics, and difficulties associated with pain measurement. To assess pain in patients suffering from acute and chronic wounds, a 1-day descriptive study was conducted involving 50 patients, 26 with acute wounds and 24 with chronic wounds, served by the wound clinic of a university hospital. Patients responded to questions regarding onset, location, type, and intensity of pain using the Visual Analog and Visual Reporting Scales and to statements about aggravating and relieving factors and overall impact on their quality of life using a 5-point scale where 5 = totally agree and 1 = completely disagree. Results showed pain was commonly mild to moderate (41 patients, 88%), located in and around wound (43 patients, 93.5%), occurred most frequently during dressing change (30 patients, 65%), and was relieved by medications (39 patients, 84.8%) and positioning (17 patients, 37%). The most commonly affected quality of life variables were physical activity (40 patients, 87% of patients) and social functioning (23 patients, 50%). Controlling wound pain can play a major role in improving patient quality of life.
Platelet functions in lymphatic filariasis patients
(Academic Press Inc., 2024) Sushil Surtani; Jyotsna Kailashiya; Mumtaz Ahmad Ansari; Debabrata Dash; Awadesh Kumar Yadav; Ashutosh Kumar
Filariasis is a chronic disease where parasitic worms survive in human hosts even for decades and lead to complications like lymphedema and elephantiasis. Despite the persistent existence of filarial parasites in human hosts, fatal and thrombotic complications are not known, unlike other parasitic diseases like malaria. This suggests that filarial parasites might be affecting the host's platelet functions. This study was conducted to examine platelet functions in confirmed filariasis patients and healthy controls. Results showed that filariasis patients had larger platelets, inhibited aggregation, and slower speed of aggregation, compared to controls. However, in vivo markers of platelet activation and degranulation (beta thromboglobulin and soluble P-selectin) were not affected. Observations suggested that there is increased platelet turnover, cellular apoptosis and inhibited platelet functions in filariasis patients compared to controls. Platelet function inhibition was not associated with the duration of disease, lymphedema-affected organs, or gender of patients. This study confirms that filarial parasites modulate platelet functions in human hosts. © 2023 Elsevier Inc.
Raised blood markers of chronic inflammation confer poor prognosis in gallbladder cancer
(Wolters Kluwer Medknow Publications, 2025) Shivam Gaba; Mallika Tewari; Mumtaz Ahmad Ansari
Background: Various pre-operative blood inflammatory markers and ratios have been used to prognosticate different cancers. We investigated a few and correlated it with Disease-Free Survival (DFS) and Overall Survival (OS) in Gallbladder Cancer (GBC) patients. Methods: Neutrophil to Lymphocyte ratio (NLR), Platelet to Lymphocyte ratio (PLR), Lymphocyte to Monocyte ratio (LMR), C-Reactive Protein (CRP), Lymphocyte to CRP ratio (LCR), modified Glasgow Prognostic Score (mGPS), CRP to Albumin ratio (CAR), and D dimer values were measured in 139 GBC patients (50 surgically resected and 89 not-operated). Results: Significantly higher risk of mortality was observed in patients with the high NLR, PLR, CRP, CAR, mGPS, and D dimer with hazard ratio of 6.935 (4.048 to 11.881), 17.101 (7.652 to 38.218), 5.916 (3.167 to 11.049), 5.532 (3.157 to 9.695), 6.912 (4.249 to 11.245), and 1.810 (1.203 to 2.725), respectively, on Univariate analysis. High LMR and LCR had significantly lower risk of mortality with hazard ratio of 0.066 (0.026 to 0.166) and 0.183 (0.11 to 0.303), respectively. Upon multivariate analysis, only high NLR, PLR, and mGPS remained statistically significant with adjusted hazard ratio of 8.278 (2.046 to 33.492), 3.907 (1.236 to 12.356), and 3.350 (1.796 to 6.249), respectively. Conclusions: High NLR, PLR, and mGPS were significantly and independently associated with higher risk of mortality. © 2025 Journal of Cancer Research and Therapeutics.
Role of Bovine Colostrum Dressing on Chronic Non-Healing Wounds in Comparison to Conventional Dressing: A Case-Control Study
(SAGE Publications Inc., 2024) Vikas Mandloi; Tuhina Banerjee; Aditya Sharma; Arvind Pratap; Mumtaz Ahmad Ansari; Vivek Srivastava
Colostrum has been shown to be suitable for oral and/or topical applications. Colostrum decreases the amount of discharge from wounds and also accelerates healing, leading to a decrease in the number of dressings. In this study, 40 patients with chronic non-healing wounds were divided into two groups, considering the inclusion and exclusion criteria. Group I included 15 patients with conventional dressings, and Group II included 25 patients with added topical colostrum dressings. All patients were assessed at the time of presentation and after 21 days. The results of the present study indicate that colostrum powder dressings may be used as an adjunct in the management of chronic non-healing wounds. © The Author(s) 2024.
Triple inguinal hernia: Rare clinical presentation
(BMJ Publishing Group, 2020) Vivek Srivastava; Pratik K. Jha; Awgesh Kumar Verma; Mumtaz Ahmad Ansari
[No abstract available]
Vulvar aggressive angiomyxoma: a surgical challenge
(BMJ Publishing Group, 2021) Vivek Srivastava; Pratik K. Jha; Awgesh Kumar Verma; Mumtaz Ahmad Ansari
Aggressive angiomyxoma is a rare and locally aggressive mesenchymal tumour, predominantly occurring in women of reproductive age group. The term aggressive is attributed to the infiltrative nature and frequent local recurrences. They arise commonly from the vulvovaginal region, perineum or pelvis and are usually misdiagnosed as other common entities in these regions. Radiological investigations aid in the diagnosis and planning of surgery. However, the final diagnosis in most of the cases is established by histopathological examination. We herein report a case of a middle-aged woman presenting with recurrent large right vulvar mass highlighting the surgical challenges posed by its intrapelvic extension. © BMJ Publishing Group Limited 2021. No commercial re-use. See rights and permissions. Published by BMJ.