Browsing by Author "Nadeem A. Ansari"

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Amadori glycated proteins: Role in production of autoantibodies in diabetes mellitus and effect of inhibitors on non-enzymatic glycation
(International Society on Aging and Disease, 2013) Nadeem A. Ansari; Debabrata Dash
Nonenzymatic glycation of macromolecules, especially proteins leading to their oxidation is increased in diabetes mellitus due to hyperglycemia and play an important role in associated complications of the disease. The glycation primarily occurs at intrachain lysine residues of proteins and results in the formation of an early stage stable product as Amadori-lysine which undergo further irreversible chemical reactions to form advanced glycation endproducts. This review deals with the role of Amadori modified proteins in pathogenesis of diabetes. We aim to explain immunogenicity of Amadori-glycated proteins, which might be involve in production of serum autoantibodies in the diabetic patients, and effect of inhibitors on the glycation process.
Clinical usefulness of a modified imunoelectrophoresis method for detection of myeloma immunoglobulins
(Nova Science Publishers, Inc., 2011) Nadeem A. Ansari; M. Owais; Usha
Immunoelectrophoresis is a clinically prevalent and sensitive technique used for isotyping of multiple myeloma. Immunoglobulin heavy and light chain types are known as potential biomarkers for the disease. In the present study, we used a modified immunoelectrophoresis method to detect the immunoglobulins of myeloma patients. Immunoglobulins of myeloma patients showed sharp precipitin bands while that of normal healthy subjects showed no characteristic bands of myeloma. The method proved to be rapid and cost effectivewith no loss of sensitivity for detection of precipitin bands corresponding to immunoglobulin heavy and light chains of myeloma patients. © 2011 Nova Science Publishers, Inc. All rights reserved.