Browsing by Author "Namrata"

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Design, synthesis, and biological evaluation of some 2-(3-oxo-5,6-diphenyl-1,2,4-triazin-2(3H)-yl)-N-phenylacetamide hybrids as MTDLs for Alzheimer's disease therapy
(Elsevier Masson s.r.l., 2024) Digambar Kumar Waiker; Akash Verma; T.A. Gajendra; Namrata; Anima Roy; Pradeep Kumar; Surendra Kumar Trigun; Saripella Srikrishna; Sairam Krishnamurthy; Vincent Jo Davisson; Sushant Kumar Shrivastava
Inspite of established symptomatic relief drug targets, a multi targeting approach is highly in demand to cure Alzheimer's disease (AD). Simultaneous inhibition of cholinesterase (ChE), β secretase-1 (BACE-1) and Dyrk1A could be promising in complete cure of AD. A series of 18 diaryl triazine based molecular hybrids were successfully designed, synthesized, and tested for their hChE, hBACE-1, Dyrk1A and Aβ aggregation inhibitory potentials. Compounds S-11 and S-12 were the representative molecules amongst the series with multi-targeted inhibitory effects. Compound S-12 showed hAChE inhibition (IC50 value = 0.486 ± 0.047 μM), BACE-1 inhibition (IC50 value = 0.542 ± 0.099 μM) along with good anti-Aβ aggregation effects in thioflavin-T assay. Only compound S-02 of the series has shown Dyrk1A inhibition (IC50 value = 2.000 ± 0.360 μM). Compound S-12 has also demonstrated no neurotoxic liabilities against SH-SY5Y as compared to donepezil. The in vivo behavioral studies of the compound S-12 in the scopolamine- and Aβ-induced animal models also demonstrated attanuation of learning and memory functions in rats models having AD-like characteristics. The ex vivo studies, on the rat hippocampal brain demonstrated reduction in certain biochemical markers of the AD brain with a significant increase in ACh level. The Western blot and Immunohistochemistry further revealed lower tau, APP and BACE-1 molecular levels. The drosophilla AD model also revealed improved eyephenotype after treatment with compound S-12. The molecular docking studies of the compounds suggested that compound S-12 was interacting with the ChE-PAS & CAS residues and catalytic dyad residues of the BACE-1 enzymes. The 100 ns molecular dynamics simulation studies of the ligand-protein complexed with hAChE and hBACE-1 also suggested stable ligand–protein confirmation throughout the simulation run. © 2024 Elsevier Masson SAS
Genetic variability and association studies for blast disease and yield traits in F2 population of Cross Hur 3022 × TETEP in rice (Oryza sativa L.)
(Plant Archives, 2019) Namrata; R.P. Singh; Ramlakhan Verma; Prashant Bisen; Manish Kumar; Arsode Pandurang; Madhu Choudhary
Analysis of variance revealed significant differences for the traits studied except, weight of panicle and length breadth ratio of grain, indicating the existence of high genetic variability among individuals of F2 population. Less difference was observed between phenotypic coefficients of variation (PCV) and genotypic coefficients of variation (GCV) for all the characters studied, indicating absence of considerable effect of the environment. Higher value for both GCV and PCV were observed for number of effective tillers per plant, weight of panicle, number of filled grains per panicle, grain yield per plant, disease severity per cent and area under disease progress curve. High heritability with high genetic advance observed for number of filled grains per panicle, number of unfilled grains per panicle, spikelet fertility percent and area under disease progress curve hence selection will be highly effective for these traits. Number of effective tillers per plant, number of filled grains per panicle, plant height and spikelet fertility % showed significant positive correlation with grain yield per plant, thus performing selection for these traits will be resulted into improvement of grain yield per plant. Leaf blast infection cause significant decrease in grain yield per plant because both blast disease related traits (disease severity % and area under disease progress curve) in this study showed significant negative correlation with the grain yield per plant. © 2019 Plant Archives. All rights reserved.
Inheritance of blast disease resistance in the cross hur 3022 x tetep of rice (Oryza sativa l.)
(Editorial board of Journal of Experimental Biology and Agricultural Sciences, 2019) Namrata; R.P. Singh; Ramlakhan Verma; Prashant Bisen; Prakash Singh; Basavaraj Teli
An experiment was carried out using six generations (P1, P2, F1, F2, B1 and B2) of cross HUR 3022 x Tetep in rice at BHU, Varanasi during year 2017-2018 to know the inheritance pattern of leaf blast disease under artificial inoculation with LB-TN-2 isolate of Magnaporthe oryzae in the field condition. The blast disease resistant cultivar ‘Tetep’ showed 9.32% disease severity, while high yielding, early maturing susceptible cultivar HUR 3022 showed 43.65% disease severity against M. oryzae. The area under the disease progress curve (AUDPC) of resistance cultivar was observed 127.95 which are significantly less than the susceptible cultivar 605.62. The F1 (HUR 3022 x Tetep) plants were observed to be resistant with an average disease severity and AUDPC are 17.54% and 224.7, respectively. The F2 population was observed to show three distinct phenotypic classes resistant, moderately resistant and susceptible with a ratio of 9:6:1, respectively. Two backcross Populations, B1 and B2 showed different response from each other during evaluation which results in the phenotypic ratio of 1R:2MR:1S in B1 and 1R:0S in B2, respectively. The results showed that blast disease resistance occurs in the cross is due to duplicate cumulative effects or polymeric gene effect of two dominant resistant genes i.e., Pi1 and Pi54. © 2019, Editorial board of Journal of Experimental Biology and Agricultural Sciences. All rights reserved.
Variability analysis and character association for sodicity tolerance in F2:3 biparental population of rice under sodic condition
(Plant Archives, 2020) Prashant Bisen; P.K. Singh; Bapsila Loitongbam; Namrata; Ashok Singmasetti; B. Sinha
The present investigation was under taken with the objectives to estimate genetic variability for sodicity related traits and its association with grain yield in F2:3 segregating population under slightly sodic condition.. The experiment was conducted at the Agricultural Research Farm of Banaras Hindu University, Varanasi (U. P.), India during kharif, 2018. Analysis of variance revealed significant differences for the traits studied indicating the existence of high genetic variability among the lines. Estimates of phenotypic coefficients of variation (PCV) were higher than genotypic coefficient of variation (GCV) for all the characters studied, indicating considerable effect of environment. Tolerant parent CSR 43 performed better than sensitive parent Pusa 44 for all the traits studied under sodic stress. CSR 43 and the F2:3 lines which showed tolerance response, recorded low shoot Na+ concentration and Na+: K+ ratio, high shoot K+ concentration, total chlorophyll content and all together respond to gain higher yield than susceptible lines. Heritability was highest among the traits studied for total chlorophyll content. Selection for shoot Na+ concentration, Na+: K+ ratio and shoot K+ concentration, total chlorophyll content will support the breeder in any breeding program for further improvement of the traits under sodic condition as these traits showed significant association with grain yield per plant in favorable negative and positive direction, respectively. The present investigation was under taken with the objectives to estimate genetic variability for sodicity related traits and its association with grain yield in F2:3 segregating population under slightly sodic condition.. The experiment was conducted at the Agricultural Research Farm of Banaras Hindu University, Varanasi (U. P.), India during kharif, 2018. Analysis of variance revealed significant differences for the traits studied indicating the existence of high genetic variability among the lines. Estimates of phenotypic coefficients of variation (PCV) were higher than genotypic coefficient of variation (GCV) for all the characters studied, indicating considerable effect of environment. Tolerant parent CSR 43 performed better than sensitive parent Pusa 44 for all the traits studied under sodic stress. CSR 43 and the F2:3 lines which showed tolerance response, recorded low shoot Na+ concentration and Na+: K+ ratio, high shoot K+ concentration, total chlorophyll content and all together respond to gain higher yield than susceptible lines. Heritability was highest among the traits studied for total chlorophyll content. Selection for shoot Na+ concentration, Na+: K+ ratio and shoot K+ concentration, total chlorophyll content will support the breeder in any breeding program for further improvement of the traits under sodic condition as these traits showed significant association with grain yield per plant in favorable negative and positive direction, respectively. © 2020 Plant Archives. All rights reserved.