Browsing by Author "Namrata Jaiswal"

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Mixed infections of begomoviruses in pumpkins with yellow vein mosaic disease in North India
(2012) Namrata Jaiswal; R.K. Saritha; D. Datta; M. Singh; R.S. Dubey; A.B. Rai; M. Rai
Yellow vein mosaic disease of pumpkin (Cucurbita moschata) is a serious problem for the cultivation of pumpkin throughout India. Symptomatic samples collected from Varanasi region of North India showed mixed infection of the three begomoviruses which could represent up to three species namely Tomato leaf curl New Delhi virus, Squash leaf curl China virus and Tomato leaf curl Palampur virus. © 2012 Copyright Taylor and Francis Group, LLC.
Phytochemicals and antioxidative enzymes defence mechanism on occurrence of yellow vein mosaic disease of pumpkin (Cucurbita moschata)
(Springer Verlag, 2013) Namrata Jaiswal; M. Singh; R.S. Dubey; V. Venkataramanappa; D. Datta
Pumpkin (Cucurbita moschata) samples showing yellow vein mosaic disease in Varanasi region were identified with begomovirus infection using PCR amplification. A sequencing analysis of the full genome revealed that it is a strain of Tomato leaf curl Palampur virus (GenBank ID. FJ931537). Phytochemical composition and antioxidative enzyme levels were compared in infected and healthy plants. The study revealed that the amount of total protein declined in the infected leaves but elevated up to 135 % in the fruits of infected plants, whereas vitamin C and antioxidants declined in infected leaves as well as fruits. There was substantial increase in total phenol content in leaves (72 %) and fruits (300 %) of infected plants. In infected samples, substantial increase in activities of superoxide dismutases (SOD), ascorbate peroxidase (APX), guaiacol peroxidase (GPX) and catalase (CAT) was observed as compared to the uninfected control plants. The native PAGE showed alterations in the intensities of isozyme bands in the infected plants. The APX, GPX, CAT, SOD and glutamate dehydrogenase (GDH) bands were intense in the infected plants, whereas the GR isozyme showed reduced intensity in diseased plants. © The Author(s) 2012.