Browsing by Author "Nandini Sarkar"

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Biophysical characterization of fibroblast growth factor homologous factor-1b (FHF-1b): Sodium dodecyl sulfate promotes two state folding
(Bentham Science Publishers B.V., 2008) Vikash Kumar Dubey; Bishal Kumar Singh; Nandini Sarkar; Monu Pande; Medicherla Venkata Jagannadham
The current article describes the biophysical characterization and folding studies of fibroblast growth factor homologous factor-1b (FHF-1b) in comparison with acidic fibroblast growth factor (FGF-1). Our data indicates that FHF-1 is significantly more stable than FGF-1. The folding mechanism of these two proteins seems to be different although they share high degree of sequence and structural similarity. FHF-1 unfolds through stable intermediate state while unfolding of FGF-1 is two-state. Interestingly, low concentration of sodium dodecyl sulfate (SDS) drives the folding pathway of FHF-1b to two-state. © 2008 Bentham Science Publishers Ltd.
Modeled structure of trypanothione reductase of Leishmania infantum
(The Biochemical Society of the Republic of Korea, 2008) Bishal K. Singh; Nandini Sarkar; M.V. Jagannadham; Vikash K. Dubey
Trypanothione reductase is an important target enzyme for structure-based drug design against Leishmania. We used homology modeling to construct a three-dimensional structure of the trypanothione reductase (TR) of Leishmania infantum. The structure shows acceptable Ramachandran statistics and a remarkably different active site from glutathione reductase(GR). Thus, a specific inhibitor against TR can be designed without interfering with host (human) GR activity.
Understanding the language of vitamin C
(2009) Nandini Sarkar; Pramod Kumar Srivastava; Vikash Kumar Dubey
Vitamin C (L-ascorbate) is a good antioxidant. Because of its water soluble nature it can work both inside and outside the cells to combat free radical damage. It has several applications starting from application in cancer therapeutics to treatment of common cold. Human, primates and guinea pigs can not synthesize this nutrient and must have to take this nutrient with diet. The current review brings together information available about the applications of Vitamin C with emphasis on antioxidant property and application in cancer therapy. The contradicting reports about application of Vitamin C in cancer therapy are also discussed. © 2009 Bentham Science Publishers Ltd.