Browsing by Author "Navneet Kumar"

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Cadmium toxicity in plants and alleviation through seed priming approach
(Springer, 2021) Navneet Kumar; Vivek Kumar; Bandana Bose; Rajesh Kumar Singhal
Heavy metals (Cd) contamination of soil and water due to anthropogenic activity, causing toxicity/stress, has potential to turn down the crop productivity and quality globally. Cd affects the fundamental and crucial processes of plants from seed germination to grain maturity, among them antioxidant defense and photosynthesis are prime one. Recent development in various disciplines of agricultural and biological sciences such as molecular breeding, integrative multiomics, and advance agronomical technology involved in heavy metal tolerance, although they are limited to lab conditions or less popular among farmers fields. Therefore, seed priming is promising and versatile approach to heavy metal stress tolerance. Seed priming with various organic and inorganic salts (CaCl2, Mg (NO3)2, proline), plant growth regulators (auxin, gibberellins, salicylic acid), showed the promising results in counteracts the effect of Cd. Seed priming involve in improvements of seed germination, seedling establishment, antioxidant defense, water and mineral nutrition, and carrying stress memory in progenies. To consider the above points, this review summarizes the effect of Cd toxicity on plant system and mineral nutrition. We also provide a glance of seed priming technology in respect to Cd stress tolerance at physiological, biochemical and molecular levels at different plant growth stages. © 2021, Indian Society for Plant Physiology.
Exploration of Neuroprotective Properties of a Naturally Inspired Multifunctional Molecule (F24) against Oxidative Stress and Amyloid β Induced Neurotoxicity in Alzheimer’s Disease Models
(American Chemical Society, 2022) Yash Pal Singh; Navneet Kumar; Khushbu Priya; Brijesh Singh Chauhan; Gauri Shankar; Saroj Kumar; Gireesh Kumar Singh; Saripella Srikrishna; Prabha Garg; Gourav Singh; Geeta Rai; Gyan Modi
The pathological hallmarks of Alzheimer’s disease (AD) are manifested as an increase in the level of oxidative stress and aggregation of the amyloid-β protein. In vitro, in vivo, and in silico experiments were designed and carried out with multifunctional cholinergic inhibitor, F24 (EJMC-7a) to explore its neuroprotective effects in AD models. The neuroprotection ability of F24 was tested in SH-SY5Y cells, a widely used neuronal cell line. The pretreatment and subsequent co-treatment of SH-SY5Y cells with different doses of F24 was effective in rescuing the cells from H2O2 induced neurotoxicity. F24 treated cells were found to be effective in the reduction of cellular reactive oxygen species, DNA damage, and Aβ1–42 induced neurotoxicity, which validated its neuroprotective effectiveness. F24 exhibited efficacy in an in vivo Drosophila model by rescuing eye phenotypes from degeneration caused by Aβ toxicity. Further, computational studies were carried out to monitor the interaction between F24 and Aβ1–42 aggregates. The computational studies corroborated our in vitro and in vivo studies suggesting Aβ1–42 aggregation modulation ability of F24. The brain entry ability of F24 was studied in the parallel artificial membrane permeability assay. Finally, F24 was tested at doses of 1 and 2.5 mg/kg in the Morris water maze AD model. The neuroprotective properties shown by F24 strongly suggest that multifunctional features of this molecule provide symptomatic relief and act as a disease-modifying agent in the treatment of AD. The results from our experiments strongly indicated that natural template-based F24 could serve as a lead molecule for further investigation to explore multifunctional therapeutic agents for AD management. © 2021 American Chemical Society
Exploring the recognition behavior of a fluorescein-based probe towards the significant detection of Cu2+ and Zn2+ ions: Experimental and computational studies
(Elsevier B.V., 2025) Navneet Kumar; Pawan Kumar Sada; Amit Kumar Kundan; Amit Bar; Amanpreet Kaur Jassal; Surya Prakash Rai; Vipendra Kumar Singh; Neha Garg; Alok Kumar Singh; Ankit Kumar Singh; Sumit Mohan Kumar; Laxman Singh; Abhishek Rai
A new fluorescein hydrazone (FA1) has been synthesized employing fluorescein hydrazide and 3,5-diiodosalicylaldehyde and its complete physiochemical characterization have been carried out. A single crystal XRD studies of FA1 has been performed. Among the tested metal ions FA1 significantly detects Cu2+ and Zn2+ in EtOH/H2O (8:2, v/v)). There is an emergence of a new absorbance peak at λmax 431 nm with Cu2+ ions. A “turn-on” behavior in fluorescence at λem510 nm was observed with Zn2+ ions owing to chelation enhanced fluorescence. This act of spectrophotometric changes and naked eye color variation from colorless to yellow is because of coordination with the metal ions rather than most common opening of spirolactum ring. FA1-Cu2+ and FA1-Zn2+ensembles display reversible behavior with EDTA2− ions. In a study on latent fingerprint detection using powder compounds, it was found that FA1 and FA1–Zn2+ showed excellent adherence to finger ridges and produced clear features without any background stains. The Hirshfeld surface and fingerprint analysis of FA1 offer a detailed examination of pairwise interactions between atoms. Additionally, topological analysis of FA1 has been conducted using NCI, AIM, ELF, and LOL methodologies. Alamar assay of FA1 on HEK-293 cell lines exhibited biocompatibility with minimal cytotoxicity. Hence, they were effectively applied for live cell imaging for intracellular sensing of Cu2+ and Zn2+ in HEK-293 cells. © 2025 Elsevier B.V.
Micro-nutrient seed priming: A pragmatic approach towards abiotic stress management
(Springer Singapore, 2020) Vivek Kumar; Rajesh Kumar Singhal; Navneet Kumar; Bandana Bose
Global population is increasing presently at a rate of 1.08% per year and that represents about an increase in 82 million people yearly. Over two centuries sevenfold increment in global population has magnified human's influence towards natural environment and disturbed its stability. Likewise, different abiotic and biotic stresses and climate change phenomenon are also responsible for catastrophic losses of the food value and productivity in agriculturally important crops. Moreover, stresses or adverse conditions lower the quality of food by curtailing the essential nutrient content in food grains. Among these essential nutrients, micro-nutrients hold the most important position for the optimum metabolic and cellular functions of living organisms. Living organism suffers from many kinds of disease/adverse conditions due to the absence of these micro-nutrients. Therefore, the optimum allocation of nutrition to plant is an important factor for improving the yield and quality food production. To diminish these losses in crops, many techniques were developed by the agriculturist, which helped in the increase in quality and production under the various adverse situations. Among them, seed priming is one of the cheap, reliable and cost-effective technique to ameliorate the effects of diverse stresses and improve food quality and production in important agriculture crops. Considering these facts, this chapter depicts the role of different micro-nutrients in plant system and responses of the plant under different adverse climatic situations. Furthermore, it will also illustrate the management of these adverse climatic conditions through the micro-nutrient seed priming (MNSP). © Springer Nature Singapore Pte Ltd. 2020. All rights reserved.
Physiological, biochemical, and molecular mechanisms of gasotransmitter-mediated heavy metal stress tolerance in plants
(CRC Press, 2021) Vivek Kumar; Akash Hidangmayum; Ankita Singh; Rekha Sodani; Basant Kumar Dadrwal; Navneet Kumar; Surendra Kumar Chaudhary; Brijesh Kumar Chaudhary; S.P. Kushwaha; Jyoti Chauhan; Hirdayesh Anuragi; Udit Nandan Mishra; Devidutta Lenka; Rajesh Kumar Singhal
[No abstract available]