Browsing by Author "Navneet Kumar Dubey"

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Clinical Spectrum of Non-motor Symptoms in Correlation with Quality of Life in Parkinson’s Disease and Atypical Parkinsonism: Evidence in Reaching Consensus
(SAGE Publications Inc., 2025) Madhusudan Rajendrakumar Tapdia; Anand Kumar; Ajay Kumar Yadav; Varun Kumar Singh; Abhishek Pathak; Rameshwar Nath Chaurasia; Vijay Nath Mishra; Navneet Kumar Dubey; Neetu Rani Dhiman; Monika Shailesh; Deepika Srivastava Joshi
Background: Non-motor symptoms (NMS) are frequently overlooked, yet they significantly contribute to the progression of Parkinson’s disease (PD) or atypical parkinsonism (AP), which include multiple system atrophy (MSA), progressive supranuclear palsy (PSP). Moreover, discrepancies exist in non-motor symptom scale (NMSS) scores for AP and PD, and no consensus has yet been reached. Purpose: We evaluated and compared the NMS and their association with life quality in patients with AP and PD. Methods: This cross-sectional observational report at a single-centre enrolling 204 patients (155 PD, 49 AP (27 MSA), and 22 PSP) from a tertiary care hospital’s movement disorder clinic. We used Movement Disorder Society Unified Parkinson’s Disease Rating Scale (MDS UPDRS)-III and modified Hoehn and Yahr (H&Y) to compute a motor score and disease severity, respectively. We assessed patients’ mental capabilities, such as cognitive impairment, through a Mini-Mental State Examination (MMSE). Meanwhile, the NMSS determined the NMSs. Quality of life (QoL) was estimated by PD Questionnaire-39 (PDQ-39). Results: We observed insignificant differences between the PD and atypical parkinsonian syndrome (APS) groups based on disease duration and gender. Worsened motor disability and disease severity were observed in AP (PSP>MSA) (P <.001). The mean NMSS scores for PD, PSP and MSA were 23.7 ± 27.9, 47.6 ± 41.3 and 65.6 ± 35.5, respectively (P <.05). MSA had a comparatively high score for sexual, cardiovascular and urinary domains, while PSP scored higher for memory/attention domains. In contrast, PD group revealed significantly lower scores for perceptual and sexual domains. Conclusion: Compared to PD, NMS was severe and highly prevalent among AP (MSA > PSP), which could be confirmed through the prevalence of sexual cardiovascular and urinary domains in MSA, while attention and mood/cognition, and sleep in PSP. © The Author(s) 2025.
Evidence in Reaching Consensus in Usage of Mandibular Advancement Device for Pre- and Post-obstructive Sleep Apnea Treatment with Blood and Salivary Inflammatory Biomarkers Profiles
(Springer, 2024) Thakur Prasad Chaturvedi; Pooja Priyadarshani; Vipul Kumar Sharma; Ishita Shrivastava; Deepak Singh; Surendra Pratap Mishra; Navneet Kumar Dubey
Purpose: To date, inconsistencies in biological sample-based biomarkers and processing protocols exist for determining obstructive sleep apnea (OSA), characterized by recurrent upper airway collapse. Hence, in this study, we investigated blood and salivary inflammatory biomarker profiles in pre- and post-MAD treated OSA patients. Methods: We corroborated OSA characteristics in 12 patients through polysomnography, which were treated with customized titratable MAD. Saliva and blood samples were obtained to determine inflammatory and stressed states through salivary α-amylase and cortisol. serum levels of CRP, IL-6, and TNF-α were also measured. Results: Levels of salivary alpha-amylase (12.86 ± 7.81 to 10.73 ± 6.60) p = 0.001 were significantly reduced with no significant difference between cortisol levels in the morning (19.79 ± 15.67 to 21.09 ± 17.97, p = 0.647), afternoon (26.73 ± 38.50 to 22.47 ± 19.36, p = 0.566), and evening (10.51 ± 7.86 to 8.02 ± 6.14, p = 0.054). Additionally, CRP (2.65 ± 0.88 to 2.19 ± 0.60) p = 0.010, IL-6 (3.12 ± 1.92 to 2.61 ± 1.50) p = 0.009, and TNF-α (40.43 ± 98.84 to 38.18 ± 100.82) p = 0.026 were significantly suppressed. Conclusion: MAD exerted anti-inflammatory, anti-stress & depression-exerting impacts in OSA patients, possibly via hypothalamic–pituitary–adrenal (HPA) axis. In our opinion, MAD is an economical alternative compared to positive airway pressure (PAP) in mild-moderate OSA, while in severe cases, a synergistic approach of MAD and PAP may be considered. © The Author(s), under exclusive licence to Springer Nature Singapore Pte Ltd. 2024.
Transcriptomic analysis reveals distinct molecular signatures and regulatory networks of osteoarthritic chondrocytes versus mesenchymal stem cells during chondrogenesis
(Palacky University Olomouc, 2025) Tsungyu Lin; Viraj Krishna Mishra; Rajni Dubey; T. P. Chaturvedi; A. Shankar Narayan; Hsuwei Fang; Lungwen Tsai; Navneet Kumar Dubey
Background. Recent regenerative studies imply conflicting results on knee osteoarthritic (OA) chondrocytes and mes-enchymal stem cells (MSC)-mediated cartilage constructs in terms of compressive properties and tensile strength. This could be attributed to different gene expression patterns between MSC and OA chondrocytes during chondrogenic differentiation. Therefore, we analyzed differentially expressed genes (DEGs) between OA and MSC-derived chondro-cytes using bioinformatics tools. Methods. We downloaded and analyzed the GSE19664 dataset from the Gene Expression Omnibus to identify DEGs. DAVID was used to perform Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses, while a protein-protein interaction network of DEGs was constructed through the Search Tool for the Retrieval of Interacting Genes (STRING) and identified hub genes by CytoHubba. Results. A total of 43 DEGs identified (15 downregulated and 28 upregulated) were found to be deregulated between OA and MSC-derived chondrocytes. KEGG analysis revealed the enrichment of complement and coagulation cascades and other pathways among the studied chondrocytes. The pathway enrichment identified top KEGG, gene ontology biological process, molecular function, and cellular component. The hub networks identified the top 5 hub genes involved in chondrogenesis, including CLU, PLAT, CP, TIMP3, and SERPINA1. Conclusions. Our results identified significant genes involved in chondrogenesis. These findings provide new avenues for exploring the genetic mechanism underlying cartilage synthesis and novel targets for preclinical intervention and clinical treatment. © 2025 The Authors.