Browsing by Author "Neelam Tia"

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Association of oxidative stress and endothelial dysfunction in hypertension
(Academic Press Inc., 2020) Pritee Chaudhary; Anand Pandey; Chandra Shekhar Azad; Neelam Tia; Manish Singh; Indrajeet Singh Gambhir
Background: Biomarkers of oxidative stress (OS) are involved in the pathophysiology of hypertension (HTN) and endothelial dysfunction is also related to HTN. Still, a significant association of OS, as well as endothelial function, remains unclear in HTN. Methods: Totalling 222 North Indian peoples aged 18–80 participated in the study. Of these participants, 74 were elderly hypertensive subjects (age ≥60 years), and 128 were normotensive subjects (age ≥60 years-control I; n = 74, and <60 years-control II; n = 74). OS was assessed by measurement of total oxidant status (TOS) and total antioxidant status (TAS) using a colorimetric and automated method developed by Erel O. Endothelial dysfunction was assessed by measurement of flow-mediated dilation (FMD) using doppler ultrasound system. Results: TOS and OSI were significantly increased and TAS and FMD significantly decreased in patients with HTN as compared to control I and control II. The increase in the level of TOS and a decrease in the level of TAS and FMD were also evident with advancing age. FMD was negatively correlated with TOS and positively correlated with TAS. Conclusion: Decreased TAS level, increased TOS level reflect OS that may be the reason for reduced FMD in elderly hypertensive patients. © 2019 Elsevier Inc.
Characterisation of bioactive metabolites from endophytic bacterial isolate
(Research Journal of BioTechnology, 2017) Moti Lal; Neelam Tia; Shiv Kr. Verma; Debashis Dutta; Debnath Das Mira
The aim of this study was to isolate and identify endophytic bacteria from stems and root of adhathoda beddomei and to evaluate their antibacterial and antifungal properties. The present investigation was carried out to analyse the morphological, physiological, biochemical and cultural characteristics diversity in the bacterial endophyte of two species of adhathoda beddomei, a total of 10 bacterial. Endophytes were isolated from tissues, stem, root. The antimicrobial and antifungal activity was tested against Escherichia coli. Pseudomonas aeroginosa, Staphylococcus aureus, Penicillium chrysogenum, Lactobacillus lactis, Bacillus subtilis and Candida albican. Minimum inhibitory concentration (MIC) of crude extract against performed microorganisms was determined. NMR, FTIR, element and mass analysis of fractionated crude extract were carried out for empirical formula and functional group. © 2017, Research Journal of BioTechnology. All rights reserved.
Role of Forkhead Box O (FOXO) transcription factor in aging and diseases
(Elsevier B.V., 2018) Neelam Tia; Alok Kumar Singh; Poorti Pandey; Chandra Shekhar Azad; Pritee Chaudhary; Indrajeet Singh Gambhir
Fork head box O (FOXO) transcription factor is a key player in an evolutionarily conserved pathway. The mammalian FOXO family consists of FOXO1, 3, 4 and 6, are highly similar in their structure, function and regulation. To maintain optimum body function, the organisms have developed complex mechanisms for homeostasis. Importantly, it is well known that when these mechanisms dysregulate it results in the development of age-related disease. FOXO proteins are involved in a diverse cellular function and also have clinical significance including cell cycle arrest, cell differentiation, tumour suppression, DNA repair, longevity, diabetic complications, immunity, wound healing, regulation of metabolism and thus treatment of several types of diseases. By the combinations of post-translational modifications FOXO's serve as a ‘molecular code’ to sense external stimuli and recruit it as to specific regions of the genome and provide an integrated cellular response to changing physiological conditions. Akt/Protein kinase B a signaling pathway as a main regulator of FOXO to perform a diverse function in organisms. The present review summarizes the molecular and clinical aspects of FOXO transcription factor. And also elaborate the interaction of FOXO with the nucleosome remodelling complex to target genes, which is essential to cellular homeostasis. © 2017
rs2253310 and rs4946936 common variants of FOXO3 gene in octogenarians and cancer: a pilot study in north India
(Springer Science and Business Media Deutschland GmbH, 2021) Neelam Tia; Moti Lal; I.S. Gambhir
Background: Healthy aging perceives human longevity probably due to carrying the defensive genes. Forkhead box O (FOXO) transcription factors provide the most convincing example of a conserved genetic pathway at the point between aging and cancer. This pilot study was performed to examine the single nucleotide variants rs2253310 and rs4946936 of the Forkhead box O 3 (FOXO3 gene) in octogenarians and gastrointestinal tract (GIT) cancer patients in the north Indian population. Main body: In silico mutational analysis of the FOXO3 gene in 25 participants. Two single nucleotide variants (SNVs) g.7556C>G (rs2253310) heterozygous and g.122284T>C (rs4946936) homozygous observed and reported previously. However, there is a common association of these SNVs in different ethnic groups. No significant differences in the genotype and allele frequencies for the study groups observed. Short conclusion: This study observes two single nucleotide variants, g.7556C>G (rs2253310) and g.122284T>C (rs4946936), of the FOXO3 gene in the study groups which influence human longevity. Longevity-associated FOXO3 variants may be associated with GIT cancer in the north Indian population. As a result, looking for genes linked to longevity will lead to discovering new cancer targets. Further studies with a large population are necessary to elucidate the role of the FOXO3 gene in octogenarians. © 2021, The Author(s).