Browsing by Author "Neera Yadav"

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Allelic sequence variation in the Sub1A, Sub1B and Sub1C genes among diverse rice cultivars and its association with submergence tolerance
(Nature Research, 2020) Anuradha Singh; Yashi Singh; Ajay K. Mahato; Pawan K. Jayaswal; Sangeeta Singh; Renu Singh; Neera Yadav; A.K. Singh; P.K. Singh; Rakesh Singh; Rajesh Kumar; Endang M. Septiningsih; H.S. Balyan; Nagendra K. Singh; Vandna Rai
Erratic rainfall leading to flash flooding causes huge yield losses in lowland rice. The traditional varieties and landraces of rice possess variable levels of tolerance to submergence stress, but gene discovery and utilization of these resources has been limited to the Sub1A-1 allele from variety FR13A. Therefore, we analysed the allelic sequence variation in three Sub1 genes in a panel of 179 rice genotypes and its association with submergence tolerance. Population structure and diversity analysis based on a 36-plex genome wide genic-SNP assay grouped these genotypes into two major categories representing Indica and Japonica cultivar groups with further sub-groupings into Indica, Aus, Deepwater and Aromatic-Japonica cultivars. Targetted re-sequencing of the Sub1A, Sub1B and Sub1C genes identfied 7, 7 and 38 SNPs making 8, 9 and 67 SNP haplotypes, respectively. Haplotype networks and phylogenic analysis revealed evolution of Sub1B and Sub1A genes by tandem duplication and divergence of the ancestral Sub1C gene in that order. The alleles of Sub1 genes in tolerant reference variety FR13A seem to have evolved most recently. However, no consistent association could be found between the Sub1 allelic variation and submergence tolerance probably due to low minor allele frequencies and presence of exceptions to the known Sub1A-1 association in the genotype panel. We identified 18 cultivars with non-Sub1A-1 source of submergence tolerance which after further mapping and validation in bi-parental populations will be useful for development of superior flood tolerant rice cultivars. © 2020, The Author(s).
Correction to: PLGA-Quercetin Nano-Formulation Inhibits Cancer Progression via Mitochondrial Dependent Caspase-3,7 and Independent FoxO1 Activation with Concomitant PI3K/AKT Suppression (Pharmaceutics, (2022), 14, 7, (1326), 10.3390/pharmaceutics14071326)
(Multidisciplinary Digital Publishing Institute (MDPI), 2024) Neera Yadav; Amit Kumar Tripathi; Amna Parveen; Shama Parveen; Monisha Banerjee
In the published publication [1], there was an error regarding the affiliation for Neera Yadav. In addition to affiliation 1, the updated affiliation should include: Molecular and Human Genetics Lab, Department of Zoology, University of Lucknow, Lucknow 226007, India. Neera Yadav did not act as the corresponding author any more. Shama Parveen and Monisha Banerjee (the corresponding author) were not included as authors in the original publication [1]. The corrected Author Contributions statement appears here. The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated. Shama Parveen: investigation, formal analysis, methodology; Monisha Banerjee: project administration, funding acquisition, resources, supervision, writing—review and editing. The authors also acknowledge Ratan Singh Ray, CSIR-Indian Institute of Toxicology Research (IITR), Lucknow, India, for the synthesis of nanoparticles; Birbal Sahni Institute of Palaeosciences (BSIP), Lucknow, for the SEM facility; Centre of Excellence, Higher Education Government of Uttar Pradesh for the cell culture facility at the Molecular and Human Genetics Laboratory, Department of Zoology, University of Lucknow, Lucknow-226007, India. The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor and Editor-in-Chief. The original publication has also been updated. © 2024 by the authors.
From QTL to variety-harnessing the benefits of QTLs for drought, flood and salt tolerance in mega rice varieties of India through a multi-institutional network
(Elsevier Ireland Ltd, 2015) Renu Singh; Yashi Singh; Suchit Xalaxo; S. Verulkar; Neera Yadav; Shweta Singh; Nisha Singh; K.S.N. Prasad; K. Kondayya; P.V. Ramana Rao; M. Girija Rani; T. Anuradha; Y. Suraynarayana; P.C. Sharma; S.L. Krishnamurthy; S.K. Sharma; J.L. Dwivedi; A.K. Singh; P.K. Singh; Nilanjay; N.K. Singh; Rajesh Kumar; S.K. Chetia; T. Ahmad; M. Rai; P. Perraju; Anita Pande; D.N. Singh; N.P. Mandal; J.N. Reddy; O.N. Singh; J.L. Katara; B. Marandi; P. Swain; R.K. Sarkar; D.P. Singh; T. Mohapatra; G. Padmawathi; T. Ram; R.M. Kathiresan; K. Paramsivam; S. Nadarajan; S. Thirumeni; M. Nagarajan; A.K. Singh; Prashant Vikram; Arvind Kumar; E. Septiningshih; U.S. Singh; A.M. Ismail; D. Mackill; Nagendra K. Singh
Rice is a staple cereal of India cultivated in about 43.5Mha area but with relatively low average productivity. Abiotic factors like drought, flood and salinity affect rice production adversely in more than 50% of this area. Breeding rice varieties with inbuilt tolerance to these stresses offers an economically viable and sustainable option to improve rice productivity. Availability of high quality reference genome sequence of rice, knowledge of exact position of genes/QTLs governing tolerance to abiotic stresses and availability of DNA markers linked to these traits has opened up opportunities for breeders to transfer the favorable alleles into widely grown rice varieties through marker-assisted backcross breeding (MABB). A large multi-institutional project, "From QTL to variety: marker-assisted breeding of abiotic stress tolerant rice varieties with major QTLs for drought, submergence and salt tolerance" was initiated in 2010 with funding support from Department of Biotechnology, Government of India, in collaboration with International Rice Research Institute, Philippines. The main focus of this project is to improve rice productivity in the fragile ecosystems of eastern, northeastern and southern part of the country, which bear the brunt of one or the other abiotic stresses frequently. Seven consistent QTLs for grain yield under drought, namely, qDTY1.1, qDTY2.1, qDTY2.2, qDTY3.1, qDTY3.2, qDTY9.1 and qDTY12.1 are being transferred into submergence tolerant versions of three high yielding mega rice varieties, Swarna-Sub1, Samba Mahsuri-Sub1 and IR 64-Sub1. To address the problem of complete submergence due to flash floods in the major river basins, the Sub1 gene is being transferred into ten highly popular locally adapted rice varieties namely, ADT 39, ADT 46, Bahadur, HUR 105, MTU 1075, Pooja, Pratikshya, Rajendra Mahsuri, Ranjit, and Sarjoo 52. Further, to address the problem of soil salinity, Saltol, a major QTL for salt tolerance is being transferred into seven popular locally adapted rice varieties, namely, ADT 45, CR 1009, Gayatri, MTU 1010, PR 114, Pusa 44 and Sarjoo 52. Genotypic background selection is being done after BC2F2 stage using an in-house designed 50K SNP chip on a set of twenty lines for each combination, identified with phenotypic similarity in the field to the recipient parent. Near-isogenic lines with more than 90% similarity to the recipient parent are now in advanced generation field trials. These climate smart varieties are expected to improve rice productivity in the adverse ecologies and contribute to the farmer's livelihood. © 2015 Elsevier Ireland Ltd.
PLGA-Quercetin Nano-Formulation Inhibits Cancer Progression via Mitochondrial Dependent Caspase-3,7 and Independent FoxO1 Activation with Concomitant PI3K/AKT Suppression
(Multidisciplinary Digital Publishing Institute (MDPI), 2022) Neera Yadav; Amit Kumar Tripathi; Amna Parveen; Shama Parveen; Monisha Banerjee
Quercetin is one of the most important plant flavanols, having several pharmacological and biological uses. Quercetin (Q) is an extremely hydrophobic phytochemical and has poor intracellular absorption, which makes its use limited. Present research demonstrates that quercetin-loaded PLGA nanoparticles (PLGA-QNPs) could overcome its low hydrophilicity and improve its anti-cancer potential. PLGA nanoparticles loaded with Q were prepared by the solvent evaporation technique and its anticancer activity was examined in vitro as well as in vivo. The cell viability was assessed through MTT assay and apoptosis was assayed through Hoechst-PI and EB/AO double staining followed by mitochondrial damage through Mito-tracker RMX-Ros. Gene expression was examined through RT-PCR. Cell cycle arrest in G2/M phase was analyzed through FACS. The results obtained revealed that PLGA-QNPs significantly reduced the viability of human cervical and breast cancer cell lines. PLGA-QNPs induced apoptosis in human cervical cancer cells in a dose dependent manner. The gene expression of PI3K/AKT was down-regulated and FoxO1 was upregulated in PLGA-QNP-treated cells, which showed a high expression level of active Caspase-3 and 7, which are responsible for apoptosis. In addition, PLGA-QNPs reduced the average number of tumors and prolonged the tumor latency period in DMBA-induced mammary adenocarcinoma SD rats. These findings suggest that PLGA-QNPs inhibit cervical and breast cancer progression via mitochondrial dependent Caspase-3 and 7 and mitochondrial independent FoxO1 activation with concomitant suppression of the PI3K/AKT pathway. For future studies, we suggest that potential druggability efficacy and clinical development of anticancer PLGA-QNPs need to be evaluated intensely for successful anticancer drug development. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.