Browsing by Author "O.P. Tiwari"

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Adaptogenic anti-stress activity of standardised extract of Marselia minuta L
(2009) O.P. Tiwari; S.K. Bhattamisra; P.N. Singh; Vikas Kumar
Marsilea minuta (Mm) was investigated on a 14-day mild, unpredictable and inescapable foot shock stress (FSS) induced perturbations in behaviour (depression), suppressed male sexual behaviour and cognitive dysfunction in albino rats. Gastric ulceration, and adrenal gland and spleen weights were also used as the stress indices. Panax ginseng (PG) was used as the standard adaptogenic agent for comparison. FSS induced marked gastric ulceration, significant increase in adrenal gland weight with concomitant decrease in spleen weight. Chronic stress also suppressed male sexual behaviour, induced behavioural depression (Porsolt's swim despair test and learned helplessness test) and cognitive dysfunction (attenuated retention of learning in active and passive avoidance tests). All these FSS induced perturbations were attenuated dose dependently by Mm (100, 200 and 400 mg/kg, p.o.) and PG (100 mg/kg, p.o.). The results indicate that Mm has significant anti-stress activity, qualitatively comparable to PG, against a variety of behavioural and physiological perturbations induced by chronic stress, which has been proposed to be a better indicator of clinical stress than acute stress, and may indicate adaptogenic activity.
Anti-inflammatory properties of BHUx, a polyherbal formulation to prevent atherosclerosis
(2004) Yamini B. Tripathi; M. Mallikarjuna Reddy; R.S. Pandey; J. Subhashini; O.P. Tiwari; B.K. Singh; P. Reddanna
BHUx is a polyherbal formulation consisting of water-soluble fractions of five medicinal plants (Commiphora mukul, Terminalia arjuna, Boswellia serrata, Semecarpus anacardium and Strychnos nux vomica). The present study was undertaken to evaluate its antioxidant and antiinflammatory effects. BHUx, standardized by HPLC fingerprinting and filtered through 0.2 μm filter paper, was employed for different studies under in vivo and in vitro conditions. Under in vivo conditions, BHUx significantly reduced inflammation in the carrageenan-induced rat paw oedema model of inflammation, suggesting its anti-inflammatory properties. In order to test the mechanism of action of BHUx, further in vitro studies were undertaken on cumene-hydroperoxide-induced lipid peroxidation (CHP) in liver homogenate, LPS-induced NO production in peritoneal macrophages and on key enzymes of arachidonic acid cascade, involved in the mediation of inflammation. Under the conditions, BHUx showed concentration-dependent inhibition of CHP-induced lipid peroxidation in liver homogenate, suggesting its antioxidant properties. Similarly the potent anti-inflammatory effects of BHUx are evident by (a) preferential inhibition of COX-2 (IC50 for COX-2 = 80 μg/ml and IC50 for COX-1 = 169 μg/ml), (b) low ratios in the IC50 values of COX-2/COX-1 (0.47), (c) decreased production of NO in LPS-induced peritoneal macrophages and (d) inhibition of 5-LOX (IC50 = 795 μg/ml). BHUx also showed a preference for inhibiting 15-lipoxygenase (IC50 = 44 μg/ml), a key enzyme implicated in LDL oxidation. These studies suggest that BHUx is acting mainly at three levels, i.e., as a potent natural antioxidant, by reduction of key inflammatory mediators of arachidonic acid cascade and by preventing 15-LOX-mediated LDL oxidations, to prevent atherosclerosis.
Antioxidant properties of different fractions of tubers from Pueraria tuberosa Linn
(2007) Nidhi Pandey; J.K. Chaurasia; O.P. Tiwari; Yamini B. Tripathi
Pueraria tuberosa Linn. (PT), Leguminosae, is a perennial climber, growing throughout tropical parts of India. In the Ayurvedic system of medicine, it is used as a drug of choice to manage pain, inflammation and other related diseases. The antioxidant potency of P. tuberosa was investigated for the first time. Total antioxidant capacity was determined using an ABTS*+ assay. Lipid peroxidation was assessed in terms of thiobarbituric acid-reactive substances by using egg-yolk homogenates as lipid-rich media. Superoxide radical-scavenging was measured using riboflavin-light-nitro blue tetrazolium (NBT) assay. Hydroxyl radical trapping potential was determined by evaluating hydroxyl radical induced deoxyribose degradation using thiobarbituric acid method. In order to assess the metal chelation property, hydroxyl radical induced deoxyribose degradation was evaluated in the absence of ethylenediaminetetraacetic acid. Both hexane and methanol fractions inhibited lipid peroxidation and also chelated the iron, showing potent antioxidant property. © 2007 Elsevier Ltd. All rights reserved.
Green synthesis, characterization and in vitro biological studies of quercetin complexes with Zn (II) acetate and N^N Moiety
(Research Journal of Pharmacy and Technology, 2021) Tanu Srivastava; S.K. Mishra; O.P. Tiwari; Kavindra Nath Tiwari; Pradeep Kumar; Jitendra Kumar; Amit Kumar Singh; Angaraj Singh; Ashutosh Dwivedi
Green synthesis of two coordination complexes of zinc acetate with N^N moiety on quercetin, which is a flavonoid is carried out. The complexes were obtained in high yields (> 97%) by grinding methods without the involvement of any solvent. Neither catalyst nor any additives were needed to perform the reactions. It was characterized by FT-IR, UV-Vis, NMR, HRMS, and elemental analysis. Antioxidant activity was done through the DPPH method which was compared with ascorbic acid and ligand (Quercetin). The study reveals that Complex 1 (IC50 163.093µg/ml) has significant free radical scavenging activity as compared to complex 2 (IC50 258.683µg/ml). Biological activity was performed against microbes (E. coli and S. aureus). MIC value of complex 1 (15.50µg/ml E. coli, 7.18µg/ml S. aureus) was found more significant as compared to complex 2 (22.51µg/ml E. coli, 18.62µg/ml S. aureus) and quercetin. © RJPT All right reserved.
Some anisotropic non-static perfect fluid cosmological models in general relativity
(1982) S.R. Roy; O.P. Tiwari
The authors have derived perfect fluid cosmological models that are anisotropic, non-static and have homogeneous distributions of density and pressure. Various physical properties of the models have been explored.