Browsing by Author "P. Maiti"

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Chitosan coated PLA nanoparticles for ophthalmic delivery: Characterization, in-vitro and in-vivo study in rabbit eye
(2010) Ramesh C. Nagarwal; P.N. Singh; S. Kant; P. Maiti; J.K. Pandit
The objective of the present study is to develop a poly (D, L-lactic acid) (PLA) nano-carrier for topical ocular applications. PLA nanoparticles (PLA-NPs) with 5-fluorouracil were prepared using varying concentration and molecular weight of PLA to regulate the particle size. The dimension and shape of nanoparticles were verified by using dynamic light scattering (DLS), atomic force microscope (AFM) and scanning electron microscope (SEM). Ex-vivo permeation study was conducted by goat and rabbit excised cornea. In-vivo experiment was conducted in rabbit eye and 5-FU concentration was measured in aqueous and vitreous humor by HPLC. In-vitro experiments indicated a diffusion controlled release of 5-FU. No significant interaction was observed in between mucin and PLA NPs that measured in terms of viscosity change. Ex-vivo permeation was significantly higher with rabbit cornea as compared to goat cornea. PLA and CH-PLA DNPs showed increased level of 5-FU as comparison to 5-FU solution. In-vivo study showed significantly higher concentration in case of uncoated and CH coated PLA nanoparticles in rabbit eye as compared to free 5-FU solution. PLA nanoparticle was found non-irritant in nature by modified Draize test. Copyright © 2010 American Scientific Publishers All rights reserved.
Mesoporous Fe3O4 nanoparticle: A prospective nano heat generator for thermo-therapeutic cancer treatment modality
(Elsevier B.V., 2023) S.K. Shaw; A. Sharma; J. Kailashiya; Santosh K. Gupta; Sher Singh Meena; D. Dash; P. Maiti; N.K. Prasad
In the present work, we have synthesized Fe3O4 MNPs with a mesoporous feature by the solvothermal process. Through iteration of experiments, we found that the specific solvent helped in stabilizing the mesoporous structure. We have used techniques, such as transmission electron microscopy (TEM) and high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM) to study the structural features of the sample. The material demonstrated effective heating under an alternating magnetic field (AMF), and the concentration of aqueous ferro-fluid influences the same. The experimental intrinsic loss power (ILP) value of ∼ 5.30 ± 0.25 nHm2kg−1 is suggestively higher than the commercially available ferro-fluids and equivalent to previously reported values for iron oxide MNPs. The material demonstrated an impressive heating performance under near infra-red (NIR) irradiation with a concentration as small as 0.1 mg/mL. The photoluminescence (PL) spectroscopy has allowed corroborating the efficient heating behaviour of the material under NIR irradiation. An effective decrease in SiHa cells viability was observed after NIR exposure with concentration of 250 and 500 μg/mL of MNPs. We believe this work will pave a new pathway in the area of biomaterials thermo-therapeutic cancer treatment with improved efficacy via mesoporous structure, efficient heating under NIR exposure and high ILP value. © 2023 Elsevier B.V.
MnFe2O4 nano-flower: A prospective material for bimodal hyperthermia
(Elsevier Ltd, 2022) S.K. Shaw; J. Kailashiya; Santosh K. Gupta; C.L. Prajapat; Sher Singh Meena; D. Dash; P. Maiti; N.K. Prasad
Magnetic nanoparticles (MNPs) have emerged as efficient materials for thermo-therapeutic modalities viz. magnetic fluid hyperthermia (MFH) and photo-thermal therapy (PTT). Nevertheless, the quest to further enhance the effectiveness of such materials is a major challenge to their successful endeavour. In the present work, we have synthesised MnFe2O4 nano-flowers utilising solvo-thermal method to be employed as nano-heat generators during MFH and PTT. The material, in addition to effective heating under an alternating magnetic field (AMF), demonstrated impressive heating ability under near infra-red (NIR) irradiation. Various techniques, such as, X-ray diffraction (XRD), transmission electron microscopy (TEM), X-ray photo-electron spectroscopy (XPS) and Mössbauer spectroscopy were used to study the structural features and chemical composition of the sample. The photoluminescence spectroscopy (PLS) has further corroborated the efficient heating behaviour of the sample under NIR irradiation. The observed intrinsic loss power value of 5.31 ± 0.079 nH m2 kg−1 is significantly higher than the commercially available ferro-fluids and comparable to previously reported values for iron oxide nano-flowers. A substantial SiHa cells death during photo-thermal treatment was noticed at a concentration of 500 μg/mL of MnFe2O4 nano-flowers after exposure to 808 nm laser of 0.66 W cm-2 power density for only 10 min. However, at the same concentration of nanoflowers (i.e. 500 μg/mL) and exposure time (i.e. 10 min) during MHT with SiHa cells, a significant decrease in cell viability was not observed. © 2021 Elsevier B.V.
Polymeric nanoparticulate system: A potential approach for ocular drug delivery
(2009) Ramesh C. Nagarwal; Shri Kant; P.N. Singh; P. Maiti; J.K. Pandit
Various efforts in ocular drug delivery have been made to improve the bioavailability and to prolong the residence time of drugs applied topically onto the eye. The potential use of polymeric nanoparticles as drug carriers has led to the development of many different colloidal delivery vehicles. Drug loaded polymeric nanoparticles (DNPs) offer several favorable biological properties, such as biodegradability, nontoxicity, biocompatibility and mucoadhesiveness. These submicron particles are better than conventional ophthalmic dosage forms to enhance bioavailability without blurring the vision. DNPs have been shown to be amenable to targeting of the drug to the site of action, leading to a decrease in the dose required and a decrease in side effects. Additionally, DNPs can be fabricated by simple techniques with better physical stability than liposomes. This unique combination of properties makes DNPs a novel polymeric drug delivery device, which fulfils the requirements for ophthalmic application. This review discusses the polymeric nanoparticles, physiochemical characterization, fabrication techniques, therapeutic significances, patented technology of nanoparticles and future possibility in the field of ocular drug delivery. Crown Copyright © 2009.
γ-Fe2O3 nanoflowers as efficient magnetic hyperthermia and photothermal agent
(Elsevier B.V., 2021) S.K. Shaw; J. Kailashiya; A. Gangwar; S.K. Alla; Santosh K. Gupta; C.L. Prajapat; Sher Singh Meena; D. Dash; P. Maiti; N.K. Prasad
Recent reports on the magnetic nanoparticles (MNPs) as an efficient and alternative photothermal agent have excited the researchers worldwide. While MNPs have been explored well for high heating performance during magnetic hyperthermia (MHT), their full potential is yet to be explored as an efficient photothermal agent. In addition, the simultaneous exposure of alternating magnetic field (for MHT) and near infrared irradiation (for photothermal therapy PTT) can drastically enhance the heating behaviour of MNPs. In the present work we explored microwave assisted polyol method to get γ-Fe2O3 nanoflowers. The use of sodium acetate in varying amounts, as an alkali source, allowed the modification of structural and magnetic properties leading to the formation of nanoflower with high heating performance during MHT and PTT. Role of defects in γ-Fe2O3 nanoflowers were investigated using photoluminescence spectroscopy which highlighted distinct role of oxygen vacancies and surface states. The nanoflowers with better crystallinity and relatively higher coercive field performed well during MHT. The observed high intrinsic loss power value of 15.21 ± 0.34 nHm2Kg-as significantly higher than the commercially available ferrofluids and previously reported values for nanoflowers. During PTT, the therapeutic temperature of 42 °C was achieved for the aqueous suspension with a concentration as low as 100 µg/mL which demonstrates the superiority of γ-Fe2O3 nanoflowers as an efficient PTT agent. © 2021 Elsevier B.V.