Browsing by Author "P.S. Yadav"

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Introgression of the high grain protein gene Gpc-B1 in an elite wheat variety of Indo-Gangetic Plains through marker assisted backcross breeding
(Elsevier B.V., 2014) Manish K. Vishwakarma; V.K. Mishra; P.K. Gupta; P.S. Yadav; H. Kumar; Arun K. Joshi
Grain protein content (GPC) in wheat has been a major trait of interest for breeders since it has enormous end use potential. In the present study, marker-assisted backcrossing (MABC) was successfully used to improve GPC in wheat cultivar HUW468. The genotype Glu269 was used as the donor parent for introgression of the gene Gpc-B1 that confers high GPC. In a segregating population, SSR marker Xucw108, with its locus linked to Gpc-B1 was used for foreground selection to select plants carrying Gpc-B1. Background selection, involving 86 polymorphic SSR markers dispersed throughout the genome, was exercised to recover the genome of HUW468. For eliminating linkage drag, markers spanning a 10. cM region around the gene Gpc-B1 were employed to select lines with a donor segment of the minimum size carrying the gene of interest. Improved lines had significantly higher GPC and displayed 88.4-92.3 per cent of the recurrent parent genome (RPG). For grain yield, selected lines were at par with the recurrent parent HUW468, suggesting that there was no yield penalty. The whole exercise of transfer of Gpc-B1 and reconstitution of the genome of HUW468 was completed within a period of two and half years (five crop cycles) demonstrating practical utility of MABC for developing high GPC lines in the background of any elite and popular wheat cultivar with relatively higher speed and precision.(. http://creativecommons.org/licenses/by-nc-nd/3.0/). © 2014 The Authors.
Marker-assisted improvement of grain protein content and grain weight in Indian bread wheat
(Springer Netherlands, 2016) Manish K. Vishwakarma; B. Arun; V.K. Mishra; P.S. Yadav; H. Kumar; Arun K. Joshi
The leading Indian wheat cultivar ‘HUW234’ produces grain with relatively low protein content (GPC) and thousand grain weight (TGW). Therefore, marker-assisted backcrossing was used to improve these two important traits by introducing favorable genes/alleles from cv. ‘Glu133’, which harbors both alleles for both GPC (Gpc-B1) and TGW. Foreground selection for GPC was achieved using microsatellite markers Xucw108 linked with Gpc-B1 and Xgwm297 linked with TGW. Background selection applied to support recovery of the recurrent parent genotype was based on 86 genomically distributed microsatellites. A selected BC2F1 plant was the progenitor of 15 BC2F4 families, in which representation of the cv. HUW234 genome ranged from 89.5 to 93.0 %. The new derivatives of HUW234 were comparable in yield to the original cultivar, but with both significantly higher GPC and heavier kernels. © 2015, Springer Science+Business Media Dordrecht.
Validation of molecular markers for stem rust resistance and identification of suitable wheat germplasm targeting Eastern Gangetic plains of India
(Indian Society of Genetics and Plant Breeding, 2015) P.S. Yadav; V.K. Mishra; B. Arun; R. Chand; A.K. Joshi
Five molecular markers were used to identify stem rust resistance genes, namely, Sr2, Sr22, Sr25/Lr19, Sr50 and SrWeb in eight diverse wheat lines from CIMMYT, Mexico and India that included three popular cultivars of north eastern Gangetic plains of India. One of the CIMMYT line, PMWBIR4 carried all the above mentioned five Sr genes, while other lines showed different combinations. The identified wheat genotypes may be useful in stem rust resistance breeding. Validation of linked markers for rust resistance suggested that the markers can be deployed in marker assisted molecular breeding program. © 2015, Indian Society of Genetics and Plant Breeding. All rights reserved.