Browsing by Author "Pitambara"

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An insight into the biodiversity and biotechnology of Fusarium species
(Elsevier, 2025) Jeetu Narware; Prachi Singh; Pitambara; Shraddha Bhaskar Sawant; Devanshu Dev; Harikesh Bahadur Singh
Fusarium species is one of the major fungi that poses challenges for food and nutritional security, as this fungus can able to cause up to 100% crop loss in many crop plants. Fusarium spp. have a wide host range and can produce a variety of symptoms in their hosts, including rot, canker, blights, and wilt. Because of the great biodiversity of the fungal species and its being soil-borne in nature, it can be linked to a variety of soil types and disseminated worldwide. Managing the fungus can be extremely challenging, leading to significant crop losses and financial losses. The fungus can survive for several years with the formation of chlamydospores or perennating structures in the soil and seeds. Therefore, it is imperative to investigate the distribution and diversity of this fungus in order to comprehend its nature and mechanism. Fusarium spp. are ubiquitous, have different ecological niches, and are soil-borne, which makes it difficult to study and manage the fungus. In light of these difficulties, Fusarium poses problems; hence, biotechnology interventions are vital for precise and timely identification and diagnosis of the fungus as well as for pathogen management. Plant pathologists have used a variety of biotechnological tools, including RNA interference (RNAi), HIGS, VIGS, genome analysis, and sequencing techniques, to better comprehend nature and biodiversity. The fungus Fusarium is worth studying in detail and gathering as much information as possible for the scientific community to better manage the diseases produced by these species due to their expanding host range and geographical presence. © 2025 Elsevier Inc. All rights reserved.
High-Throughput sequencing: A tool to curb banana diseases of quarantine importance
(Springer Nature, 2025) Prachi Singh; Priya Bhargava; Sawant Shraddha Bhaskar; Jeetu Narware; Sudha Nandni; Pitambara; Devanshu Dev
The number of plant diseases of economic importance is rapidly growing, and their status is shifting from minor to major diseases. To restrict the transboundary spread of these diseases quarantine plays an important role. Many diseases that affect banana fall under either domestic or international quarantines. The use of High-Throughput sequencing (HTS) technology is transforming research and trying to address the problem of banana diseases. HTS tools are widely used in genomics, transcriptomics, epigenomics, and metagenomics. The main benefits of HTS that make it a promising tool for further research are its applicability, sensitivity, and ease of use when working with large genomic datasets. HTS has been widely used in the precise, rapid, and early detection of Fusarium wilt of banana, including TR4, Banana mosaic, and Moko disease of banana, etc. HTS tools like Illumina, PacBio, Oxford Nanopore, and MiSeq pyrosequencing have also been used in the evolutionary study of Ralstonia solanancearum phylotypes, Banana viral diseases, delineating the Fusarium oxysporum f. sp. cubense TR4 (Foc TR4) race etiology in Panama wilt. Several studies showed the applicability of HTS tools in unravelling the mechanism of resistance, host specificity, and identification of resistance genes of banana diseases which helped in the development of integrated management strategies. This review will provide a close insight into the importance and application of HTS technology in various banana diseases of quarantine importance and the future scope of these tools to explore new possibilities of research to manage these diseases. © Koninklijke Nederlandse Planteziektenkundige Vereniging 2025.
Nanomaterial-based biosensors: a new frontier in plant pathogen detection and plant disease management
(Frontiers Media SA, 2025) Jeetu Narware; Jharjhari Chakma; Satyendra Pratap Singh; Divya Raj Prasad; Jhumishree Meher; Prachi Singh; Priya Bhargava; Shraddha Bhaskar Sawant; Pitambara; Jyoti Prakash Singh; Nazia Manzar; Abhijeet Shankar Kashyap
Nanotechnology has significantly advanced the detection of plant diseases by introducing nano-inspired biosensors that offer distinct advantages over traditional diagnostic methods. These biosensors, enhanced with novel nanomaterials, exhibit increased sensitivity, catalytic activity, and faster response times, resulting in improved diagnostic efficiency. The increasing impact of climate-induced stress and emerging plant pathogens have created an urgent demand for real-time monitoring systems in agriculture. Nanobiosensors are revolutionizing plant disease management by enabling on-site detection of pests and weeds, facilitating precise pesticide applications. This article provides a comprehensive overview of the development and application of nanobiosensors in real-time plant disease diagnosis. It highlights key innovations, such as smartphone-integrated nanozyme biosensing and lab-on-a-chip technologies. Special emphasis is placed on the detection of molecular biomarkers, demonstrating the critical role of nanobiosensors in addressing the evolving challenges of plant disease management and agricultural sustainability. © © 2025 Narware, Chakma, Singh, Prasad, Meher, Singh, Bhargava, Sawant, Pitambara, Singh, Manzar and Kashyap.