Browsing by Author "Pooja Agarwal"

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Serum cortisol and thyroid hormone levels in neonates with sepsis
(The Indian Journal of Pediatrics, 2002) B.K. Das; Pooja Agarwal; J.K. Agarwal; O.P. Mishra
Objective : To evaluate the thyroid hormone and cortisol levels in neonates with sepsis in relation to the final outcome. It was hypothesized that the hormonal level could act as some prognostic guideline. Methods : Forty nine neonates, aged 8-28 days , diagnosed as neonatal sepsis were selected for the study. Neonates below 8 days of age, 35 weeks of gestation and 2000 g of birth weight were excluded from the study. Twenty FT-AGA neonates beyond day 7 of life served as control for the study. The hormones were estimated by radioimmunoassay. Results : The neonates with sepsis had significantly higher mean serum cortisol and lower mean serum total T4 at admission as compared to healthy neonates. The mean serum total T3 level was also lower, but the difference was not statistically significant. The mean serum TSH levels were comparable in both groups. The levels normalised following recovery. Sixteen neonates succumbed to the disease process. The non-survivors had significantly lower mean total T3 and total T4 levels as compared to the survivors. Conclusion. The endocrinal abnormalities are of transient nature as a response to sepsis. Low total T3 and total T4 are the predictors of adverse outcome in neonates with sepsis.
Study of diagnostic efficacy of bronchoalveolar lavage and bronchoscopic brush cytology in clinico-radiologically suspected cases of lung malignancies
(Indian Academy of Clinical Medicine, 2018) Prashant Prakash; Pooja Agarwal; Arup Dasgupta; Khushboo Gahoi
Background: Fibre-optic bronchoscopy (FOB), along with its guided procedures such as endobronchial biopsy, bronchial brushings, bronchoalveolar lavage (BAL), transbronchial needle aspiration and transbronchial lung biopsy, is the most widely used technique for the diagnosis of lung cancer. This study was undertaken to study the diagnostic efficacy of BAL and bronchoscopic brush cytology in clinico-radiologically suspected cases of lung malignancies, and compare them with biopsy, wherever possible. Methods: FOB was performed on 130 clinico-radiologically suspected cases of lung malignancy, reporting to our tertiary care centre. The samples obtained were sent for histological and cytological examination. Results: Bronchoscopic brushing and BAL was done in all the 130 patients and biopsy was performed in 65 cases. Out of these 130 cases, 100 were diagnosed to have lung malignancies giving an overall sensitivity of 76.92% for FOB. Of these 100 cases, 55 patients were detected by BAL alone and bronchial brushing alone diagnosed 80 cases. The most common histological subtype detected was squamous cell carcinoma (57%) followed by adenocarcinoma (24%). Biopsy had a sensitivity of 93.8% as it diagnosed 61/65 cases. Conclusion: Fibre-optic bronchoscopy - along with its guided procedures - is easy, minimally invasive and is associated with few risks. We recommend that all clinico-radiologically suspected cases of lung cancers should be investigated by BAL, brushings, and endobronchial biopsy wherever possible, since using one or two of the procedures decreases the diagnostic yield of FOB. © 2018, Indian Academy of Clinical Medicine. All rights reserved.
WS2 nanoparticle integrated MWCNT as an efficient electrode material for electrochemical sensing of chloramphenicol in pharmaceutical samples
(Elsevier Inc., 2025) Pinky Sagar; Ayushi Sahrawat; Monika Srivastava; Priyanka; Pooja Agarwal; Sanjay Kumar Srivastava
Detection of an antibiotic drug chloramphenicol (CPN) in pharmaceutical samples is very essential due to its wide application in food, agriculture and healthcare sector and nanomaterials are gaining significant attention as efficient electrode materials in the realm of CPN residue diagnostics. Among these materials, graphene-based compounds stand out due to their intriguing π–π interaction properties. In this study, we present a facile hydrothermal technique for the synthesis of WS2 nanoparticles which later decorated on multiwalled carbon nanotubes (WS2-MWCNT). This nanocomposite material was then employed for the electrochemical detection of CPN simultaneously by two techniques: cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The WS2-MWCNT based sensor exhibited an impressive low limit of detection of 0.041 nM (CV) and 0.34 nM (DPV), and a remarkable sensitivity of 4.29 μAμM−1cm−2 (CV) and 0.71 μAμM−1cm−2 (DPV), along with excellent performance in terms of selectivity, and reproducibility for CPN detection. Furthermore, the sensor demonstrated successful detection of CPN in pharmaceutical and natural matrices with satisfactory results. This proposed voltammetric sensor presents an inexpensive and promising approach for applications in monitoring pharmaceutical samples, with great opportunities for its miniaturization and detection in real samples. © 2025 Elsevier B.V.