Browsing by Author "Pooja Pandey"

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Characterization of Pseudofusicoccum adansoniae, an Endophytic Fungus Residing in Photosynthetic Root of Tinospora cordifolia, a Medicinal Plant
(Springer, 2019) Ashish Mishra; Surendra K. Gond; Vijay K. Sharma; Satish K. Verma; Jitendra Kumar; Dheeraj K. Singh; Anuj Kumar; Pooja Pandey; R.N. Kharwar
An endophytic fungus isolated from photosynthetic root of Tinospora cordifolia Miers. was identified as Pseudofusicoccum adansoniae with 100% 18S ITS rRNA partial gene sequence similarity (accession JX951181). The fungus exhibited amylase, lipase and protease activities and was also able to assimilate the galactose, glucose, fructose, lactose and maltose along with l-arginine and alanine. In addition to siderophore production, the fungus was found to be tolerant against various concentrations of NO2 −, NO3 − and NaCl. The maximum biomass and metabolite yield were observed at pH range 5.4–7.0, whereas yield of secondary metabolites was highest in potato dextrose broth (36.66 ± 0.33 mg/100 ml) extracted through ethyl acetate (EtOAc). Interestingly, metabolites extracted through ethyl acetate from PDB and MEB grown cultures were found to be active against all 8 human bacterial pathogens used in this study. The isolation and characterization of P. adansoniae done in this experiment are the initial steps which pave the way for purified isolation of bioactive compounds and enzymes of immense use. © 2018, The National Academy of Sciences, India.
Fumonisins in Food and Feed: Their Detection and Management Strategies
(CRC Press, 2022) Shubhangi Srivastava; Ashok Kumar Yadav; Mousumi Ghosh; Dipendra Kumar Mahato; Madhu Kamle; Pooja Pandey; Sreemoyee Chakraborty; Pradeep Kumar
Contamination by fumonisins in agricultural crops and food-related products is affected by agro-climatic conditions. Cereals are the most often contaminated food categories. The fumonisin levels in popcorn or sweet corn, milk, meat, and eggs are generally low, while those in corn meal, bran, flour, grits, distiller grains, gluten, milling fractions, and baking mixes are quite high. Fumonisin dietary intake can result in a variety of negative consequences in agricultural and laboratory animals. From 1980-95, fumonisins were detected by thin layer chromatography on silica plates. After spraying the plate with p-anisaldehyde, followed by heating, the fumonisins develop as light to dark purple dots. Some chemical and physical methods for fumonisin reduction have been marketed entailing solvent extraction, sorting and flotation, detoxification by chemical alkalization. Mycotoxin adsorption methods have been adopted by the use of natural clay as adsorbent media in the food processing sector, resulting in the detoxification of food. © 2023 Pradeep Kumar, Madhu Kamle, and Dipendra Kumar Mahato.