Browsing by Author "Pradeep Kumar Patel"

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Antioxidant defence system in chickpea (Cicer arietinum L.): Influence by drought stress implemented at pre- and post-anthesis stage
(2012) Pradeep Kumar Patel; A. Hemantaranjan
Significant differences in the antioxidant system of the leaves of four chickpea (Cicer arietinum L.) genotypes differing in tolerance to drought were observed in a pot experiment. Drought stress was imposed at pre- and post- anthesis stage (50 and 65 days after sowing). We studied the effect of drought stress on hydrogen peroxide (H2O2) content, lipid peroxidation and antioxidant enzyme activities in four chickpea genotypes viz., Tyson, ICC 4958, JG 315 and DCP 92-3. Drought treatment significantly increased hydrogen peroxide accumulation and lipid peroxidation, which indicated the extent of oxidative injury posed by stress conditions. Enzyme activities of Superoxide Dismutase (SOD), Peroxidase (POX), Catalase (CAT) and Ascorbate Peroxidase (APX) increased in chickpea leaves as a consequence of drought conditions and all the enzyme activities were significantly higher in drought stressed genotypes i.e., Tyson and ICC 4958 than in JG 315 and DCP 92-3. Relationship between Lipid Peroxidation (LPO), Hydrogen peroxide (H2O2), Superoxide Dismutase (SOD), Catalase (CAT), Peroxidase (POX) and Ascorbate Peroxidase (APX) was determined in order to find out whether these parameters can be used as selection criteria for drought tolerance in this crop. Early drought stress (pre-anthesis drought) was found to be more damaging than the late drought stress (post-anthesis drought). © 2012 Academic Journals Inc.
Effect of salicylic acid on growth and metabolism of chickpea (Cicer Arietinum L.) Under drought stress
(2012) Pradeep Kumar Patel; A. Hemantaranjan; B.K. Sarma
Four chickpea genotypes (Tyson, ICC 4958, JG 315 and DCP 92-3) were treated with 1.0 mM and 1.5 mM salicylic acid (SA) and subjected to pre- and post flowering drought stress to analyse its influence on nitrate reductase (NR) activity, relative water content (RWC), proline and antioxidant enzymes activity (superoxide dismutase and peroxidase). Leaf RWC significantly reduced during stress at both the growth stages and ranged between 71.67-74.43% (unstressed) and 67.96-71.67% (stressed), whereas in 1.5 mM SA treated plants leaf RWC increased comparable to the control (unstressed plant). NR activity significantly reduced under stress at the post anthesis stage of growth but was maintained higher in 1.5 mM SA treated plants in all the four genotypes studied. On the other hand, activities of antioxidant enzymes superoxide dismutase (SOD) and peroxidase (POX) were upregulated by drought stress and interestingly further enhanced by 1.5 mM SA treatment. The response of SA (1.5 mM) was relatively more in ICC 4958 and Tyson cultivars of chickpea. Hence, results signify the role of SA in protecting metabolic activity along with regulating the drought response of plants.
In vitro cytotoxic potential of cow dung and expired tomato sauces-derived carbon nanodots against A-375 human melanoma cell line
(Elsevier B.V., 2024) Gaurav Gopal Naik; Reena Madavi; Tarun Minocha; Debadatta Mohapatra; Ravi Pratap; Singh Shreya; Pradeep Kumar Patel; Sanjeev Kumar Yadav; Avanish Parmar; Arjun Patra; Nishant Sudhir Jain; Swaha Satpathy; Mohsin Kazi; Muhammad Delwar Hussain; Alakh N Sahu
Converting biowaste into a functional product is put to the test by the growing amount of biowaste in the world and the environmental problems it causes. In this research study, we synthesized, characterized, and evaluated bluish-green luminescent carbon nanodots (CNDs) from cow dung and expired tomato sauces via a hydrothermal synthesis method at 160 °C for 8 h. The carbon nanodots were fabricated without additional passivating agents and exhibited good physicochemical and optical properties. The intrinsic properties of carbon nanodots were characterized using various spectral techniques. First, we evaluated the cytotoxic potential of carbon nanodots against A-375 human melanoma cell lines. This study revealed that carbon nanodots exhibited potent cytotoxicity and significantly inhibited the proliferation of A-375 cells in a dose-dependent manner. Next, we demonstrated these carbon nanodot's free radical scavenging potential by employing 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The bluish-green fluorescent carbon nanodots fabricated using a green synthesis approach have broad potential for biological applications. © 2023 The Authors
Salicylic acid improves salinity tolerance in field pea (Pisum sativum L.) by intensifying antioxidant defense system and preventing salt-induced nitrate reductase (NR) activity loss
(Agricultural Research Communication Centre, 2015) Radha Singh; A. Hemantaranjan; Pradeep Kumar Patel
To improve the antioxidant system and protect the nitrate reductase (NR) activity of two field pea genotypes (DDR 61 and HUDP 15) by seed hardening through optimum concentration salicylic acid (SA) @ 1.0 mM under salinity stress condition. Salinity was imposed by NaCl @ 50,100 and 150 mM with their corresponding EC 4.0, 8.2 and 10.6 dSm-1 respectively. Nitrate reductase (NR) activity significantly reduced under stress at the reproductive (i.e. post anthesis) stage but was maintained higher in 1.0 mM SA treated plants upto the level of 100 mM NaCl. In addition to NR, membrane stability index (MSI) also decreased significantly under salinity stress. In DDR 61 MSI was found to be more as compared to HUDP 15. On the other hand, activities of antioxidant enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT) were up-regulated by salinity stress and further enhanced remarkably by 1.0 mM SA treatment. DDR 61 genotype was found to be more responsive to SA application as compared to HUDP 15. Salt stress was found to have more damaging effects during the pre-anthesis phase than the post-anthesis phase of development. Hence, results signify the role of SA in protecting NR metabolic activity along with regulating salinity response of plants. © 2015, Agricultural Research Communication Centre. All rights reserved.
Salicylic acid induced alteration in dry matter partitioning, antioxidant defence system and yield in chickpea (Cicer arietinum L.) under drought stress
(2012) Pradeep Kumar Patel; A. Hemantaranjan
An experiment was conducted to investigate the possible involvement of exogenously applied salicylic acid (presowing seed soaking at the rate of 1.0 and 1.5 mM salicylic acid (SA) on dry matter production and its partitioning, antioxidant (proline and sugar), antioxidant enzymes i.e., superoxide dismutase, peroxidase and yield of four chickpea (Cicer arietinum L.) genotypes, viz., Tyson, ICC 4958, JG 315 and DCP 92-3 under drought stress. Drought imposed (by withholding irrigation) at 50% flowering and 50% podding phase of development. Results indicate wide variation in tolerance to drought stress amongst chickpea genotypes at both the critical stages i.e., 50% flowering and 50% podding. However, the yield and its components were higher in ICC 4958 as compared to other genotypes. It is inferred that the significant response of applied SA at the rate of 1.5 mM under drought condition was relatively more in ICC 4958 genotype in terms of antioxidant response; substantially alleviated drought induced adverse effect and yield. It is also conclude that drought stress at 50% flowering was found to be more damaging than the 50% podding phase. © 2012 Asian Network for Scientific Information.