Browsing by Author "Pradyot Prakash"

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A case report of tinea nigra from North India
(2009) Ragini Tilak; Sanjay Singh; Pradyot Prakash; Dharmendra Singh; Anil Gulati
[No abstract available]
Assessing genetic diversity of Mycobacterium tuberculosis by spoligotyping and IS6110-based restriction fragment length polymorphism in North India
(Elsevier Ltd, 2016) Jitendra Prasad Mathuria; Pragya Sharma; Pradyot Prakash; Jai Kumar Samaria; Vishwa Mohan Katoch; Shampa Anupurba
Objective/background Molecular epidemiology methods are very useful for differentiating between strains, assessing their diversity, and measuring the prevalence of the most circulating strain in an area. Various molecular typing methods using different molecular markers have been utilized worldwide, such as restriction fragment length polymorphism (RFLP), spoligotyping, Mycobacterial Interspersed Repetitive Unit – Variable Number of Tandem Repeat (MIRU-VNTR), and Double repetitive element-PCR (DRE-PCR) typing, for simultaneous detection and epidemiologic typing of Mycobacterium tuberculosis. The present study is conducted to assess the genetic diversity of M. tuberculosis by IS6110-RFLP and spoligotyping in patients attending a tertiary care hospital in eastern Uttar Pradesh, North India. Methods A total of 83 representative isolates of M. tuberculosis were included in this study. These isolates were subjected to spoligotyping and IS6110-RFLP DNA fingerprinting techniques as described previously. Results The spoligotype patterns were compared with SpolDB4.0; patterns of 64 out of 83 M. tuberculosis isolates were matched with the available data, while 19 isolates were found to be orphan, that is, absent in the SpolDB4.0 database. The majority of the M. tuberculosis strains (56.5%) belong to central Asian (32.5%), ill defined T (13.2%), and Beijing (10.8%) families. On IS6110-RFLP analysis, in 19.2% (16/83) of these isolates, IS6110 element was not found (0 copy number strains). Further, 15.6% (13/83) isolates were found to be low-copy-number strains having less than six copies of IS6110 element, and the remaining 65.0% (54/83) were multiple-copy-number strains with six or more copies of the element. On comparing the results of spoligotyping and IS-6110-RFLP, a total of 47 isolates were clustered by spoligotyping; out of these isolates, 40 were found to be unique by IS6110-RFLP. Conclusion Spoligotype analysis resulted in the grouping of a much larger number of isolates within apparently identical clusters compared with IS6110-RFLP typing, while IS6110-RFLP was not found to effectively distinguish between zero- and low-copy-number isolates. Therefore, we concluded that, in India, the use of both the techniques simultaneously for DNA fingerprinting of M. tuberculosis could be a better approach. © 2016
Assessment of Successful qRT-PCR of SARS-CoV-2 Assay in Pool Screening Using Isopropyl Alcohol Purification Step in RNA Extraction
(Hindawi Limited, 2021) Mayank Gangwar; Alka Shukla; Virendra Kumar Patel; Pradyot Prakash; Gopal Nath
The study is aimed at establishing the optimal parameters for RNA purification of pooled specimens, in SARS-CoV-2 assay. This research work evaluates the difference of extracted RNA purity of pooled samples with and without treatment with isopropyl alcohol and its effect on real-time RT-PCR. As per the protocol of the Indian Council of Medical Research (ICMR), 5 sample pools were analysed using qRT-PCR. A total of 100 pooled samples were selected for the study by mixing 50 μL of one COVID-19 positive nasopharyngeal/oropharyngeal (NP/OP) specimen and 50 μL each of 4 known negative specimens. Pool RNA was extracted using the column-based method, and 1 set of pooled extracted RNA was tested as such, while RNA of the second set was treated additionally with chilled isopropyl alcohol (modified protocol). Further, the purity of extracted RNA in both the groups was checked using Microvolume Spectrophotometers (Nanodrop) followed by RT-PCR targeting E-gene and RNaseP target. The results showed that the purity index of extracted RNA of untreated pooled specimens was inferior to isopropyl alcohol-treated templates, which was observed to be 85% sensitivity and 100% specificity. The average Cq (E gene) in the unpurified and purified pool RNA group was 34.66 and 31.48, respectively. The nanodrop data suggested that purified RNA concentration was significantly increased with an average value of 24.73±1.49 ng/uL, which might be the reason for high sensitivity and specificity. Thus, this group testing of SARS-CoV-2 cases using pools of 5 individual samples would be the best alternative for saving molecular reagents, personnel time, and can increase the overall testing capacity. However, purity of RNA is one of the important determinants to procure unfailing results, thus, this additional purification step must be included in the protocol after RNA has been extracted using commercially available kit before performing qRT-PCR. © 2021 Mayank Gangwar et al.
Burden of Pediatric Scrub Typhus at a Tertiary Care Hospital: Clinical, Biochemical, Complications and Predictors of Mortality and Morbidity
(Springer, 2024) Janani G; Pradyot Prakash; Rajniti Prasad; Abhishek Abhinay; Ankur Singh
[No abstract available]
Classification of clinical isolates of klebsiella pneumoniae based on their in vitro biofilm forming capabilities and elucidation of the biofilm matrix chemistry with special reference to the protein content
(Frontiers Media S.A., 2019) Ashish Kumar Singh; Shivangi Yadav; Brijesh Singh Chauhan; Nabarun Nandy; Rajan Singh; Kaushik Neogi; Jagat Kumar Roy; Saripella Srikrishna; Rakesh Kumar Singh; Pradyot Prakash
Klebsiella pneumoniae is a human pathogen, capable of forming biofilms on abiotic and biotic surfaces. The limitations of the therapeutic options against Klebsiella pneumoniae is actually due to its innate capabilities to form biofilm and harboring determinants of multidrug resistance. We utilized a newer approach for classification of biofilm producing Klebsiella pneumoniae isolates and subsequently we evaluated the chemistry of its slime, more accurately its biofilm. We extracted and determined the amount of polysaccharides and proteins from representative bacterial biofilms. The spatial distribution of sugars and proteins were then investigated in the biofilm matrix using confocal laser scanning microscopy (CLSM). Thereafter, the extracted matrix components were subjected to sophisticated analysis incorporating Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (NMR) spectroscopy, one-dimensional gel-based electrophoresis (SDS-PAGE), high performance liquid chromatography (HPLC), and MALDI MS/MS analysis. Besides, the quantification of its total proteins, total sugars, uronates, total acetyl content was also done. Results suggest sugars are not the only/major constituent of its biofilms. The proteins were harvested and subjected to SDS-PAGE which revealed various common and unique protein bands. The common band was excised and analyzed by HPLC. MALDI MS/MS results of this common protein band indicated the presence of different proteins within the biofilm. The 55 different proteins were identified including both cytosolic and membrane proteins. About 22 proteins were related to protein synthesis and processing while 15 proteins were identified related to virulence. Similarly, proteins related to energy and metabolism were 8 and those related to capsule and cell wall synthesis were 4. These results will improve our understanding of Klebsiella biofilm composition and will further help us design better strategies for controlling its biofilm such as techniques focused on weakening/targeting certain portions of the slime which is the most common building block of the biofilm matrix. Copyright © 2019 Singh, Yadav, Chauhan, Nandy, Singh, Neogi, Roy, Srikrishna, Singh and Prakash. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Click inspired synthesis of hexa and octadecavalent peripheral galactosylated glycodendrimers and their possible therapeutic applications
(Royal Society of Chemistry, 2019) Anand K. Agrahari; Anoop S. Singh; Ashish Kumar Singh; Nidhi Mishra; Mala Singh; Pradyot Prakash; Vinod K. Tiwari
A Cu(i)-catalyzed azide-alkyne 1,3-dipolar cycloaddition reaction (CuAAC) has been utilized for the synthesis of novel glycodendrimers containing a rigid hexapropargyloxy benzene centered core with 6- and 18-peripheral β-d-galactopyranosidic units. Structures of the novel glycodendrimers and intermediates were well elucidated using nuclear magnetic resonance and infrared spectroscopies, matrix assisted laser desorption/ionization mass spectrometry, and size-exclusion chromatography. The therapeutic evaluations of the developed glycodendrimers were investigated and they were found to have good potential as anti-bacterial, anti-biofilm, and anti-tumour agents. © The Royal Society of Chemistry and the Centre National de la Recherche Scientifique 2019.
Click inspired synthesis of: P-tert -butyl calix[4]arene tethered benzotriazolyl dendrimers and their evaluation as anti-bacterial and anti-biofilm agents
(Royal Society of Chemistry, 2020) Anand K. Agrahari; Ashish K. Singh; Anoop S. Singh; Mala Singh; Pathik Maji; Shivangi Yadav; Sanchayita Rajkhowa; Pradyot Prakash; Vinod K. Tiwari
Calixarenes with their three-dimensional structural architecture and ease of functionalization at the upper and/or lower rim are well-known for their plethora of applications in chemical, physical, biological and other disciplines. Herein, CuAAC click inspired p-tert-butylcalix[4]arene tethered benzotriazolyl dendrimers, for example, N-1, N-2 type 2-fold compound 6 and 7 'G(0)' and 6-fold compound 16 and 17 'G(1)' generation benzotriazolyl dendrimer, were devised and also evaluated for their therapeutic potential both in vitro and in vivo. The developed calix[4]arene tethered benzotriazolyl dendrimers have been characterized by standard spectroscopic analysis including NMR (1H and 13C), MS, and SEC. Among all the benzotriazolyl dendrimers developed, compound 7 was identified as the effective one which provided potential anti-bacterial and anti-biofilm activities against drug-resistant and slime producing organisms without imparting cytotoxicity to the eukaryotic systems. This journal is © The Royal Society of Chemistry and the Centre National de la Recherche Scientifique.
Correction: Quantum curcumin mediated inhibition of gingipains and mixed-biofilm of Porphyromonas gingivalis causing chronic periodontitis(RSC Advances (2018) 8 (40426–40445) DOI: 10.1039/C8RA08435A)
(Royal Society of Chemistry, 2019) Ashish Kumar Singh; Shivangi Yadav; Kavyanjali Sharma; Zeba Firdaus; Prerana Aditi; Kaushik Neogi; Monika Bansal; Munesh Kumar Gupta; Asheesh Shanker; Rakesh Kumar Singh; Pradyot Prakash
The authors regret the incorrect naming of the bacterial species Actinomycetemcomitans viscosus in the published article. It should be correctly shown as Actinomyces viscosus throughout, on pages 40427 (fifth line of last paragraph), 40436 (sixth line of “Individual isolates & mixed” section), 40437 (Table 2) and 40440 (Table 3). Also, the ATCC number of A. viscosus was incorrectly given as 29522 throughout the published article and should be correctly shown as 15987 in the following places: p. 40429 (“Bacterial strains and culture conditions” section and “Minimum inhibitory concentration determination” section), p. 40430 (“Determination of antibiofilm activity using tissue culture plate assay (TCP)” section), p. 40434 (“Antimicrobial assay of quantum curcumin against clinical isolates of Porphyromonas gingivalis and select reference strains” section and “Determination of minimum inhibitory concentration” section), p. 40435 (“Growth rate analysis” section), p. 40437 (Table 2), and on p. 40438 (caption to Fig. 7 (twice), caption to Fig. 8, and sixth line of “Discussion” section). An error was also present in the published article in the co-author name spelling for K. Sharma. The correct spelling of the author names is as shown here. The Royal Society of Chemistry apologises for these errors and any consequent inconvenience to authors and readers. © The Royal Society of Chemistry.
Cryptococcal meningitis with an antecedent cutaneous Cryptococcal lesion
(Dermatology Online Journal, 2009) Ragini Tilak; Pradyot Prakash; Chaitanya Nigam; Vijai Tilak; I.S. Gambhir; A.K. Gulati
Cutaneous cryptococcosis, caused by an encapsulated yeast, Cryptococcus neoformans, is generally associated with concomitant systemic infection. Here we report a case of primary cutaneous cryptococcosis with spread to central nervous system in an HIV seronegative young boy. In the present case, a 17-year-old boy who was suffering from a non-healing ulcer on his right great toe for 5 months, presented with the signs and symptoms of meningitis. Cryptococcus neoformans var. gattii was isolated from the CSF of the patient. Amphotericin B administration produced recovery from the meningitis as well as from the ulcer. This case study suggests that primary cutaneous cryptococcosis can be diagnosed provisionally by a simple Gram stained smear and India ink examination in order to avoid occurrence of disseminated cryptococcosis, including meningial involvement, which may have a fatal outcome. © 2009 Dermatology Online Journal.
Curcumin quantum dots mediated degradation of bacterial biofilms
(Frontiers Media S.A., 2017) Ashish K. Singh; Pradyot Prakash; Ranjana Singh; Nabarun Nandy; Zeba Firdaus; Monika Bansal; Ranjan K. Singh; Anchal Srivastava; Jagat K. Roy; Brahmeshwar Mishra; Rakesh K. Singh
Bacterial biofilm has been reported to be associated with more than 80% of bacterial infections. Curcumin, a hydrophobic polyphenol compound, has anti-quorum sensing activity apart from having antimicrobial action. However, its use is limited by its poor aqueous solubility and rapid degradation. In this study, we attempted to prepare quantum dots of the drug curcumin in order to achieve enhanced solubility and stability and investigated for its antimicrobial and antibiofilm activity. We utilized a newer twostep bottom up wet milling approach to prepare Curcumin Quantum Dots (CurQDs) using acetone as a primary solvent. Minimum inhibitory concentration against select Gram-positive and Gram-negative bacteria was performed. The antibiofilm assay was performed at first using 96-well tissue culture plate and subsequently validated by Confocal Laser Scanning Microscopy. Further, biofilm matrix protein was isolated using formaldehyde sludge and TCA/Acetone precipitation method. Protein extracted was incubated with varying concentration of CurQDs for 4 h and was subjected to SDS–PAGE. Molecular docking study was performed to observe interaction between curcumin and phenol soluble modulins as well as curli proteins. The biophysical evidences obtained from TEM, SEM, UV-VIS, fluorescence, Raman spectroscopy, and zeta potential analysis confirmed the formation of curcumin quantum dots with increased stability and solubility. The MICs of curcumin quantum dots, as observed against both select gram positive and negative bacterial isolates, was observed to be significantly lower than native curcumin particles. On TCP assay, Curcumin observed to be having antibiofilm as well as biofilm degrading activity. Results of SDS–PAGE and molecular docking have shown interaction between biofilm matrix proteins and curcumin. The results indicate that aqueous solubility and stability of Curcumin can be achieved by preparing its quantum dots. The study also demonstrates that by sizing down the particle size has not only enhanced its antimicrobial properties but it has also shown its antibiofilm activities. Further, study is needed to elucidate the exact nature of interaction between curcumin and biofilm matrix proteins. © 2017 Singh, Prakash, Singh, Nandy, Firdaus, Bansal, Singh, Srivastava, Roy, Mishra and Singh.
Cutaneous zygomycosis: A possible postoperative complication in immunocompetent individuals
(2009) Ragini Tilak; Prabhat Raina; Sanjeev Kumar Gupta; Vijai Tilak; Pradyot Prakash; Anil Kumar Gulati
Fungi in the class of zygomycetes usually produce serious infections in diabetics and immunocompromised hosts. Cutaneous zygomycosis is a less common form, with an unpredictable extent of anatomical involvement and clinical course. Here, we report two cases of primary cutaneous zygomycosis as postoperative complications in otherwise healthy females. Zygomycosis was suspected and specimens from the surgical debridement were examined by microbiological and histopathological studies for confirming the clinical diagnosis. Rapid diagnosis, liposomal amphotericin B, and proper debridement of affected tissue are necessary to avoid a fatal outcome.
Design, Synthesis, Antimicrobial Activities, and Molecular Docking Studies of New Glyco-Conjugates of Amino Acid Derivatives
(John Wiley and Sons Inc, 2025) Sajida Banoo; Zinnu Rain; Abhinetra Jagdish Bhopale; Rajnish Kumar; Pradyot Prakash; Ram Sagar; Arun Kumar Manna
The existence of MDR and XDR strains of bacteria is a serious threat to mankind. Therefore, the development of new drug molecules with different modes of action is the need of the hour. Medicinal chemists and chemical biologists around the globe are making continuous efforts to combat this challenging health issue. In this effort, we have designed and efficiently synthesized amino acid glycoconjugates of D-glucose as new glycohybrids. These novel glyconjugates were screened against Staphylococcus aureus and Staphylococcus epidermidis Gram (+) ve and Escherichia coli Gram (−) ve bacterial strains. The antibiofilm properties of these glyconjugates were also studied. Furthermore, these compounds were evaluated for their antifungal property against two fungal strains, namely Candida albicans and Candida parapsilosis. The detailed antimicrobial activity is presented here. The molecular docking of selected active glycoconjugates is carried out with bacterial gyrase and fungal α-demethylase and compared with standard drugs novobiocin and fluconazole, respectively, which revealed some interesting molecular interactions. © 2025 Wiley-VCH GmbH.
Detection and characterisation of AmpC β-lactamase-producing isolates of Acinetobacter spp. in North India
(2010) Supriya Upadhyay; Malay Ranjan Sen; Pradyot Prakash; Shampa Anupurba; Gopal Nath; Amitabha Bhattacharjee
[No abstract available]
Detection of OXA-2 group extended-spectrum-β-lactamase-producing clinical isolates of Escherichia coli from India [2]
(2007) Amitabha Bhattacharjee; Malay Ranjan Sen; Shampa Anupurba; Pradyot Prakash; Gopal Nath
[No abstract available]
Development, optimization and evaluation of curcumin loaded biodegradable crosslinked gelatin film for the effective treatment of periodontitis
(Taylor and Francis Ltd., 2018) Sheetal Chauhan; Monika Bansal; Gayasuddin Khan; Sarita K. Yadav; Ashish K. Singh; Pradyot Prakash; Brahmeshwar Mishra
Objective: Aim of the present study was to prepare curcumin (CUR) loaded biodegradable crosslinked gelatin (GE) film to alleviate the existing shortcomings in the treatment of periodontitis. Significance: Gelatin film was optimized to provide anticipated mucoadhesive strength, mechanical properties, folding endurance, and prolonged drug release over treatment duration, for successful application in the periodontitis. Methods: The film was developed by using solvent casting technique and “Design of Experiments” approach was employed for evaluating the influence of independent variables on dependent response variables. Solid-state characterization of the film was performed by FTIR, XRD, and SEM. Further, prepared formulations were evaluated for drug content uniformity, surface pH, folding endurance, swelling index, mechanical strength, mucoadhesive strength, in vitro biodegradation, and in vitro drug release behavior. Results: Solid state characterization of the formulation showed that CUR is physico-chemically compatible with other excipients and CUR was entrapped in an amorphous form inside the smooth and uniform film. The optimized film showed degree of crosslinking 51.04 ± 2.4, swelling index 138.10 ± 1.25, and folding endurance 270 ± 3 with surface pH around 7.0. Crosslinker concentrations positively affected swelling index and biodegradation of film due to altered matrix density of the polymer. Results of in vitro drug release demonstrated the capability of the developed film for efficiently delivering CUR in a sustained manner up to 7 days. Conclusions: The developed optimized film could be considered as a promising delivery strategy to administer medicament locally into the periodontal pockets for the safe and efficient management of periodontitis. © 2018 Informa UK Limited, trading as Taylor & Francis Group.
Diagnostic utility of combination of inducer and inhibitor based assay in detection of Pseudomonas aeruginosa producing AmpC β-lactamase
(2011) Supriya Upadhyay; Malay Ranjan Sen; Amitabha Bhattacharjee; Pradyot Prakash; Ram Chandra Bajpai; Shampa Anupurba
The diagnostic utility of inducer and inhibitor based assays among 214 AmpC positive isolates of Pseudomonas aeruginosa were evaluated. Of different methods, combination of ceftazidime-imipenem antagonism and boronic acid inhibition tests came up with maximum sensitivity (76%) and specificity (100%). This combination showed reliability for both inducible and non-inducible AmpC producers. © 2011 Elsevier B.V.
Differentiation of Mycobacterium tuberculosis complex from non-tubercular mycobacteria by nested multiplex PCR targeting IS6110, MTP40 and 32kD alpha antigen encoding gene fragments
(BioMed Central Ltd., 2016) Pallavi Sinha; Anamika Gupta; Pradyot Prakash; Shampa Anupurba; Rajneesh Tripathi; G.N. Srivastava
Background: Control of the global burden of tuberculosis is obstructed due to lack of simple, rapid and cost effective diagnostic techniques that can be used in resource poor-settings. To facilitate the early diagnosis of TB directly from clinical specimens, we have standardized and validated the use of nested multiplex PCR, targeting gene fragments IS6110, MTP40 and 32kD α-antigen encoding genes specific for Mycobacterium tuberculosis complex and non-tubercular mycobacteria (NTM), in comparison to smear microscopy, solid culture and single step multiplex PCR. The results were evaluated in comparison to a composite reference standard (CRS) comprising of microbiological results (smear and culture), clinical, radiological and cytopathological findings, clinical treatment and response to anti-tubercular therapy. Methods: The nested multiplex PCR (nMPCR) assay was evaluated to test its utility in 600 (535 pulmonary and 65 extra-pulmonary specimens) clinically suspected TB cases. All specimens were processed for smear, culture, single step multiplex PCR and nested multiplex PCR testing. Results: Out of 535 screened pulmonary and 65 extra-pulmonary specimens, 329 (61.5 %) and 19 (29.2 %) cases were culture positive for M. tuberculosis. Based on CRS, 450 patients had "clinical TB" (definitive-TB, probable-TB and possible-TB). Remaining 150 were confirmed "non-TB" cases. For culture, the sensitivity was low, 79.3 % for pulmonary and 54.3 % for extra-pulmonary cases. The sensitivity and specificity results for nMPCR test were evaluated taken composite reference standard as a gold standard. The sensitivity of the nMPCR assay was 97.1 % for pulmonary and 91.4 % for extra-pulmonary TB cases with specificity of 100 % and 93.3 % respectively. Conclusion: Nested multiplex PCR using three gene primers is a rapid, reliable and highly sensitive and specific diagnostic technique for the detection and differentiation of M. tuberculosis complex from NTM genome and will be useful in diagnosing paucibacillary samples. Nested multiplex PCR assay was found to be better than single step multiplex PCR for assessing the diagnosis of TB. © 2016 Sinha et al.
Dot enzyme immunoassay (Typhidot®) in diagnosis of typhoid fever in children
(2007) Pradyot Prakash; Malay Ranjan Sen; Om Prakash Mishra; Anil Kumar Gulati; Bishwa Nath Shukla; Gopal Nath
[No abstract available]
Epidemiological Distribution and Potential Risk Factors of Orientia tsutsugamushi Infection in Eastern Uttar Pradesh, India
(Hamadan University of Medical Sciences, 2021) Alka Shukla; Mayank Gangwar; Akanksha Srivastava; Sonam Rastogi; Deepak Kumar; Digvijay Singh; Rajesh Kumar; Pradyot Prakash; Gopal Nath
Background: Scrub typhus (ST) is a rickettsial infection caused by Orientia tsutsugamushi, which presents with flu like symptoms. This disease has been reported from all over India but with slight variations in its pattern. For decreasing the prevalence, preventing new incidences, and predicting the course of the ST, therefore, it is crucial to gain knowledge and perception of local risk components associated with the disease. The present study aimed to investigate the epidemiological distribution and potential risk factors of O. tsutsugamushi Infection in Eastern Uttar Pradesh (EUP), India. Methods: The serums of 211 samples were collected from the suspected cases along with the detailed information about the participants such as age, location, and place recorded in case history form (CRF). IgM estimation was performed using enzyme-linked immunosorbent assay (ELISA) assay. Results: A total of 58 samples (27.4%) out of 211 ones were found to be positive for IgM antibodies against O. tsutsugamushi bacterium. Furthermore, the results were correlated with epidemiological data such as gender, rural or urban background, pets, and occupation. The results showed that 76.7% of the study participants were from rural areas or had bushes around their houses, 88.3% of them had pets/cattle or frequent encounter with rodents at their houses, and 30.3% of them had no toilet facilities at home. Conclusions: It was concluded that the proximity to pets/cattle, having rodents in closer vicinity, residing in places surrounded by vegetation/farm/bushy areas, and following occupations involving field work increased the chances of getting bitten by mites/chiggers. Overall, Orientia tsutsugamushi prevalence increased in EUP, with respect to clinical features, disease presentation, and laboratory diagnosis can help our community to reduce the mortality caused by this infectious disease. © 2021 The Author(s); Published by Hamadan University of Medical Sciences.
Evaluation of nested PCR in diagnosis of typhoid fever
(2005) Pradyot Prakash; Om Prakash Mishra; Alok Kumar Singh; Anil Kumar Gulati; Gopal Nath
In this study, nested PCR using H1-d primers, which is specific for Salmonella enterica serovar Typhi, was compared to blood culture and the single-tube Widal test. Results indicate that nested PCR can be used as a gold standard to determine the cutoff titer of the Widal test for diagnosis of typhoid fever.