Browsing by Author "Prakash C. Misra"

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Erratum: Immunodiagnostic analysis of transgenic vegetative insecticidal protein in genetically modified crops/produce (Food Chemistry (2011) 126 (786-792))
(2011) Chandra K. Singh; Rajesh Kumar; Rajeshwar P. Sinha; Prakash C. Misra
[No abstract available]
Immunodiagnostic analysis of transgenic vegetative insecticidal protein in genetically modified crops/produce
(2011) Chandra K. Singh; Rajesh Kumar; Rajeshwar P. Sinha; Prakash C. Misra
In the process of development of insect resistant genetically modified (GM) crops and also to evaluate the consistency in the expression of toxin under field conditions, immunological assays are commonly being used. We have developed immunoassay to support labelling of vegetative insecticidal protein (Vip3A) based GM produce. The developed ELISA for measurement of Vip3A is a triple antibody sandwich procedure utilising a polyclonal capture antibody (anti-Vip3A raised in mice) and a polyclonal detection antibody (anti-Vip3A raised in rabbits) followed by use of a third HRP-conjugated antispecies antibody (goat anti-rabbit IgG). The detection limit for the developed ELISA assay was 16 ppb of Vip3A protein. The quantification range was 31-500 ppb of Vip3A protein. The assay is sensitive, quick and reproducible can be utilise in regulating GM produce. © 2010 Elsevier Ltd. All rights reserved.