Browsing by Author "Prakriti Sundar Samanta"

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Poly(N-acryloyl-l-phenylalanine) nanoparticles for potential treatment of inflammation in selective organs
(Royal Society of Chemistry, 2025) Divya Pareek; Sukanya Patra; Md Zeyaullah; Gurmeet Singh; Taniya Das; Prakriti Sundar Samanta; Aman Srikant Kudada; Anjali Ramsabad Mourya; Kirti Wasnik; Rajalaxmi Pradhan; Yitzhak Mastai; Pradip Paik
Systemic inflammation can lead to multi-organ failure. The existing anti-inflammatory agents show adverse side effects, and the present situation demands new drugs with high therapeutic efficiency. Polymeric nanoparticles based on amino acids could be one of the best alternative solutions due to their cytocompatibility and immune responses. Herein, we synthesized polymeric nanoparticles (Phe NPs) with a size of 20-30 nm using N-acryloyl-l-phenylalanine methyl ester as a precursor. The biological and immune responses of Phe NPs were found to be commanding, which was proven using immune cells (RAW 264.7 macrophages). In vitro study revealed an easy uptake of these NPs (∼98%) by the immune cells and that they can reduce inflammation by improving the immune response. In silico molecular docking results revealed that Phe NPs could potentially interact with immune cytokines such as IL-6, NF-κβ, TNF-α, COX2 and IL-1β. Phe NPs exhibit a similar type of binding and interaction as ibuprofen (IBF), which confirms its immune response to control inflammation. The anti-inflammatory response of Phe NPs was established through an in vitro inflammation model developed using LPS-stimulated RAW 264.7 macrophages. Furthermore, an LPS-induced in vivo rat model was developed, which revealed that Phe NPs are useful for the treatment of systemic inflammation. Blood-based biochemical parameters such as C-reactive protein, lactate and procalcitonin levels were determined, and the anti-inflammatory responses of Phe NPs were confirmed through RT-PCR analysis by measuring the levels of inflammatory markers such as TNF-α, IL-6 and VEGF. Finally, an in vivo systemic inflammation rat model was used to examine the systemic organs (brain, liver, kidneys, spleen, lungs and heart) before and after treatment with Phe NPs to prove their anti-inflammatory responses. H&E histological analysis of different organs further revealed that even at a low dose of 100 μg kg−1, Phe NPs are immune-responsive/protective and anti-inflammatory in nature. © 2025 The Royal Society of Chemistry.
Self-assembled amino acid-based copolymer nanoparticles for wound healing and tissue regeneration: structure studied through molecular dynamic simulation
(Royal Society of Chemistry, 2025) Sukanya Patra; Desh Deepak Yadav; Gurmeet Singh; Jyotirmayee; Prakriti Sundar Samanta; Divya Pareek; Aman Srikant Kudada; Anjali Ramsabad Mourya; Debdip Bhandary; Pradip Paik
Amino acid-based block copolymer nanoparticles with cross-linkers have garnered growing interest in recent years. However, its intricate synthesis and purification difficulties, along with stability concerns linked to intermicellar crosslinking, restrict their potential use in healthcare and therapeutic applications. Thus, the present work aimed to design amphiphilic block copolymer nanoparticles of N-acryloyl glycine and N-acryloyl-(l-phenylalanine methyl ester), i.e., p(NAG-co-NAPA)wc, without the use of a crosslinker via miniemulsion free radical polymerization. The self-assembled π-π stacking structural arrangement of the copolymer at different temperatures has been confirmed through molecular dynamics (MD) simulations, which corroborated the structural stability of the copolymer nanoparticles at physiological temperature (37 °C). The cell migration results of the p(NAG-co-NAPA)wc nanoparticles are complementary to those of the CEMA assay, revealing their tissue regeneration properties. Furthermore, the in vivo wound healing study demonstrated that within 13 days post-treatment, ∼97% of the wound can be healed, whereas for the control, it was found to be only ∼80%. Additionally, the RT-PCR results revealed that the p(NAG-co-NAPA)wc nanoparticles possess anti-inflammatory and tissue regeneration properties by downregulating TNF-α and IL-1β and upregulating PECAM-1 and VEGF-A, respectively. In conclusion, these p(NAG-co-NAPA)wc nanoparticles are paramount with an extensive clinical potential for the regeneration of acute wounds and can be used for other therapeutic applications. © 2025 The Royal Society of Chemistry.