Browsing by Author "Pralay Maiti"

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A non-covalently cross-linked self-healing hydrogel for drug delivery: characterization, mechanical strength, and anti-cancer potential
(Royal Society of Chemistry, 2024) Sheetal Jaiswal; Sandeep Kumar; Paramjeet Yadav; Krishtan Pal; Shere Afgan; Arvind Acharya; Ravi Prakash; Pralay Maiti; Rajesh Kumar
This research article introduced a novel one-pot method for synthesizing hydrogels, utilizing iron ions (Fe3+) and guar gum succinate (GGS) as cross-linkers. These hydrogels were characterized as cross-linked networks, with hydrogen bonds forming a sacrificial network and coordination bonds serving as the primary network. The reversible nature of these networks was attributed to the hydrogels’ exceptional toughness and remarkable self-healing properties. The hydrogel's chemical structure was confirmed through FTIR spectroscopy. XRD analysis highlighted the disruption of the crystalline nature of GGS upon cross-linking with Fe3+. By controlling the Fe3+ concentration, the hydrogels’ mechanical properties were tailored. Rheological measurements demonstrated mechanical and self-healing properties, while swelling studies revealed pH-dependent behavior. In vitro studies showed the hydrogels’ significant anti-proliferative effect against U-87MG (human glioblastoma) cancer cells, while remaining biocompatible with normal cell lines (HEK-293). These results indicated the potential application of these hydrogels in advancing cancer treatment strategies. © 2024 The Royal Society of Chemistry.
Allylthiourea-mediated self-healing hydrogels based on poly(vinyl alcohol): Enhanced cell viability/biocompatibility and sustained drug release
(John Wiley and Sons Inc, 2024) Paramjeet Yadav; Shere Afgan; Krishtan Pal; Sheetal Jaiswal; Pooja Goswami; Ravi Prakash; Rajesh Kumar; Biplob Koch; Pralay Maiti
Hydrogel-based materials represent promising candidates for drug delivery. In our research, we synthesized a series of hydrogels—PVATU-1 (2:1), PVATU-2 (1:1), and PVATU-3 (0.5:1)—by adjusting the poly(vinyl alcohol) (PVA) ratio while maintaining a constant ratio of allylthiourea (ATU) monomer and glutaraldehyde cross-linker, aiming to understand their impacts on gelation. These PVATU hydrogels displayed impressive swelling in neutral conditions and exhibited visible self-healing capabilities. Notably, PVATU-1 shows superior mechanical strength among the variants. Surface analysis using AFM and SEM unveiled porous structures within the hydrogels, while thermal stability tests indicated their resilience up to 200°C. DSC analysis revealed minimal variation in glass transition temperatures (Tg), affirming stability. A distinct endothermic peak around 250–350°C across all hydrogels confirmed their semi-crystalline nature, corroborated by powder XRD. Noteworthy, PVATU-1 demonstrated optimal loading and releasing efficiency for levofloxacin, with 74.5% loading and 82.8% releasing efficiency within 18 h. Biocompatibility assessments on HeLa cells affirmed the nontoxicity of PVATU hydrogel treatments. © 2024 Wiley Periodicals LLC.
Alterations in the epidermis and mucus viscosity of the carp, Cirrhinus mrigala, experimentally infected with Edwardsiella tarda
(Academic Press, 2025) Jyoti S. Singh; Usha Kumari; Ravi Prakash; Pralay Maiti; Swati Mittal; Ajay Kumar Mittal
This study investigated effects of a bacterial pathogen, Edwardsiella tarda on the epidermis and mucus viscosity of an Indian major carp, Cirrhinus mrigala. The fish were divided into three groups: a control group (no treatment), a vehicle control group (fish injected with 50 μl of phosphate-buffered saline (PBS) at day 0), and an infected group (fish injected with 50 μl of PBS containing a sublethal dose of 2.2 × 106 CFU/fish, which is 10 % of the 96-h LD50, at day 0). Alterations in the surface architecture, histology, proliferating cell nuclear antigen (PCNA) and inducible nitric oxide synthase (iNOS) expression, specific activity of lactate dehydrogenase (LDH) and succinate dehydrogenase (SDH) were studied at 2d, 4d, 6d and 8d post-infection. Microscopic examination showed hypertrophy of the epidermal epithelial cells, accompanied by disrupted and disorganized microridges, as well as exfoliation. Mucous goblet cells (MGCs) density increased significantly at an early stage of infection. Club cells exhibited degenerative changes, including vacuolization, confluence with neighbouring cells at intervals and simultaneous discharge of their contents onto the surface. A significant increase in iNOS-positive cells was detected. PCNA expression was significantly lower in infected fish, indicating reduced cell proliferation. Cutaneous mucus showed non-Newtonian behavior, with higher viscosity at low shear rates which decreased significantly in infected fish, indicating thinning and shedding under stress. E. tarda infection also caused a significant increase in LDH activity and a decrease in SDH activity. This study will provide deep insight into the host defence mechanisms and serve as a knowledge base for the establishment of early warning systems to control disease outbreaks in farmed fish. © 2025 Elsevier Ltd
Anti-cancer evaluation of quercetin embedded PLA nanoparticles synthesized by emulsified nanoprecipitation
(Elsevier B.V., 2015) Sanjeev K. Pandey; Dinesh K. Patel; Ravi Thakur; Durga P. Mishra; Pralay Maiti; Chandana Haldar
This study was carried out to synthesize quercetin (Qt) embedded poly(lactic acid) (PLA) nanoparticles (PLA-Qt) and to evaluate anti-cancer efficacy of PLA-Qt by using human breast cancer cells. PLA-Qt were synthesized by using novel emulsified nanoprecipitation technique with varying dimension of 32 ± 8 to 152 ± 9. nm of PLA-Qt with 62 ± 3% (w/w) entrapment efficiency by varying the concentration of polymer, emulsifier, drug and preparation temperature. The dimension of PLA-Qt was measured through transmission electron microscopy indicating larger particle size at higher concentration of PLA. The release rate of Qt from PLA-Qt was found to be more sustained for larger particle dimension (152 ± 9. nm) as compared to smaller particle dimension (32 ± 8. nm). Interaction between Qt and PLA was verified through spectroscopic and calorimetric methods. Delayed diffusion and stronger interaction in PLA-Qt caused the sustained delivery of Qt from the polymer matrix. In vitro cytotoxicity study indicate the killing of ~50% breast cancer cells in two days at 100. μg/ml of drug concentration while the ~40% destruction of cells require 5 days for PLA-Qt (46 ± 6. nm; 20. mg/ml of PLA). Thus our results propose anticancer efficacy of PLA-Qt nanoparticles in terms of its sustained release kinetics revealing novel vehicle for the treatment of cancer. © 2015 Elsevier B.V.
Biocompatible thermoresponsive N-isopropyl-N-(3-(isopropylamino)-3-oxopropyl)acrylamide-based random copolymer: synthesis and studies of its composition dependent properties and anticancer drug delivery efficiency
(Royal Society of Chemistry, 2022) Sourov Mondal; Archana Kumari; Kheyanath Mitra; Abhineet Verma; Satyen Saha; Biswajit Maiti; Ranjeet Singh; Partha Pratim Manna; Pralay Maiti; Hironobu Watanabe; Masami Kamigaito; Biswajit Ray
A new acrylamide monomer, N-isopropyl-N-(3-(isopropylamino)-3-oxopropyl)acrylamide (M3i), consisting of both isopropyl and isopropylamidopropyl moieties, has been synthesized from isopropylamine and N-isopropylacrylamide via an aza-Michael addition reaction followed by amidation with acryloyl chloride. The homopolymer of M3i (polyM3i) and a series of random copolymers of M3i and poly(ethylene glycol)methyl ether acrylate (PEGA: CH2 00000000 00000000 00000000 00000000 11111111 00000000 11111111 00000000 00000000 00000000 CHCO2(CH2CH2O)nMe, Mn = 480, n = 9 on average) with varying compositions have been synthesized via reversible addition-fragmentation chain transfer polymerization using 2-(dodecylthiocarbonothioylthio)-2-methylpropionic acid (DDMAT) as well as 1-phenylethyl phenyl dithioacetate (PEPD) as a RAFT agent. These polymers have been characterized by 1H NMR, FTIR, GPC, UV-Vis, fluorescence, TGDTA, DSC, DLS, and TEM techniques. A lower critical solution temperature (LCST) and glass transition temperature (Tg) for polyM3i prepared using DDMAT were observed at 17 and 133 °C, respectively, while for a polymer formed using PEPD, no LCST was observed until 0 °C and its observed Tg was found at 127.3 °C. The polymers are thermally stable up to 300 °C. Upon an increase in the M3i content in the copolymers, LCST decreases, Tg increases, and the apparent hydrodynamic diameter decreases. Moreover, the effects of concentration and the addition of urea and sodium chloride on the LCST of the copolymer with an LCST close to body temperature were studied. Owing to the incorporation of PEGA, a higher critical micellar concentration and larger TEM particle size of this copolymer were observed with respect to those of polyM3i. The usefulness of the micelles of the copolymers as nano-carriers for the drug doxorubicin was explored. The in vitro tumoricidal activity of the micelles of the doxorubicin-loaded copolymers was also assessed against Dalton's lymphoma cells. © 2022 The Royal Society of Chemistry.
Biodegradable poly(ϵ-caprolactone) as a controlled drug delivery vehicle of vancomycin for the treatment of MRSA infection
(Royal Society of Chemistry, 2016) Alok Rai; Sudipta Senapati; Shyam K. Saraf; Pralay Maiti
Biodegradable poly(ϵ-caprolactone) (PCL) is developed as a controlled drug delivery vehicle of vancomycin (VMC) with the advantage of avoiding a second surgery. The PCL-VMC hybrid, prepared through a solution route, is used as a delivery vehicle for vancomycin for controlling MRSA osteomyelitis as well as healing the cavity simultaneously in an experimental study. An in vitro study is conducted to optimize vancomycin impregnation in the PCL-VMC hybrid. An in vitro study on drug release from the hybrid material is investigated in phosphate buffer saline showing steady and sustained release of the drug. The release kinetics is fitted with several models and a non-Fickian nature is established following the Korsmeyer-Peppas model. Spectroscopic techniques and morphology observations reveal the cause of sustained release to be the strong interaction between the drug and the polymer. The results of the antibacterial assay show that the loading of vancomycin into the PCL matrix is able to maintain the activity of the pure drug. For the in vivo study, a unicortical defect is created in the metaphysis of the distal femur in rabbits. After contaminating the defect with MRSA, the 1st group of rabbits were treated with pure polymer, the 2nd group of rabbits were treated with normal saline (PBS), the 3rd group of rabbits were treated with pure VMC and in the last group of rabbits PCL-VMC was placed. Rabbits are assessed by clinical, radiological, histological, gross examination and bacterial load assays. Infection persisted throughout the period of study for both the pure polymer and PBS treated rabbits while rabbits treated with the PCL-VMC hybrid do not show any sign of infection. The VMC treated group rabbits show mild infection for the 1st week of the study; however, the infection becomes gradually more severe with time. Serial histology confirms the formation of new bone without any inflammation and necrosis for the rabbits treated with PCL-VMC. Importantly, the PCL-VMC hybrid bioadsorbs after delivery of the drug and thereby avoids the second surgery to remove the conventional implant. © The Royal Society of Chemistry 2016.
Biodegradable polymers for potential delivery systems for therapeutics
(Springer New York LLC, 2013) Sanjeev K. Pandey; Chandana Haldar; Dinesh K. Patel; Pralay Maiti
Biodegradable polymers are being extensively used with great interest in areas of nanobiotechnology such as drug delivery, diagnostics, and other applications for clinical and biomedical research covering cardiovascular diseases, diabetes, osteogenesis, cancer, and tissue engineering. Various biodegradable polymers such as poly(lactic acid), poly(lactic-co-glycolic acid), poly (ε-caprolactone), chitosan, gelatin, and poly(alkyl cyanoacrylates) have been extensively utilized as polymeric materials and devices for targeted cellular and tissue-specific clinical applications to achieve maximal therapeutic efficacy with minimal or no side effects. Recently, polymeric nanoparticles have revolutionized the area of nanobiotechnology by creating new opportunities for advancing medical science and disease treatment. Polymeric nanoparticles have the potential to act as a carrier of drugs and active constituents to targeted sites, protecting them from the environment and controlling their release rates, thereby enhancing their biological activity and decreasing the adverse side effects. This article compiles updated information regarding various biodegradable polymers, methods of preparation of biodegradable polymeric nanoparticles, and their application in therapeutic and diagnostic strategies for various diseases. This article will support research scientists and clinical physicians who are interested in the development and application of biodegradable polymeric nanoparticles as potential delivery systems for therapeutics. © 2013 Springer-Verlag Berlin Heidelberg.
Brominated Graphene as Mimetic Peroxidase for Sulfide Ion Recognition
(American Chemical Society, 2017) Shikha Singh; Kheyanath Mitra; Aparna Shukla; Rajshree Singh; Ravi Kumar Gundampati; Nira Misra; Pralay Maiti; Biswajit Ray
Brominated graphene (GBR) with ∼3% bromine content has shown novel peroxidase mimetic activity toward 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H2O2. Optimum activity has been observed at pH 4.48 and after a minimum ∼30 min of equilibration time. Among the different analytes studied using the sensor combining TMB, H2O2, and GBR in phosphate buffer of pH 4.48, the S2- ion has effectively shown a short duration of sensing (∼2 min) within the detection range of 0.04-1 mM. A calibration curve for S2- ion estimation has been constructed with the experimental linearity in 0.04-0.4 mM range and having the limit of detection (LOD) value of 25.3 μM. A standard addition experiment has validated the method. A paper strip sensor has been fabricated for successful detection of S2- ion. © 2016 American Chemical Society.
Chitosan nanoparticles of 5-fluorouracil for ophthalmic delivery: Characterization, in-vitro and in-vivo study
(2011) Ramesh Chand Nagarwal; Paras Nath Singh; Shri Kant; Pralay Maiti; Jayanta Kumar Pandit
The aim of this investigation was to develop 5-fluorouracil (5-FU) loaded chitosan nanoparticles (CH-DNPs) for ophthalmic delivery. CH-DNPs were fabricated by ionotropic gelation mechanism using chitosan (CH) and a polyanion (TPP). The nanoparticles were smooth and spherical, confirmed by scanning electron microscopy (SEM) and atomic force microscope (AFM). CH/TPP mass ratio and TPP significantly changed the particles size morphology and encapsulation efficiency. The nanoparticles size ranged from approximately 114 to 192 nm and had a positive zeta potential (30±4 mV). The encapsulation efficiency, loading capacity and recovery of DNPs were 8.12-34.32%, 3.14-15.24% and 24.22 to 67% respectively. Physical characterization was done by Fourier transform infrared (FT-IR) and X-ray diffraction (XRD). No interaction was observed in between drug and polymer and crystallinity of drug was not changed in drug loaded nanoparticles. In-vitro release study of DNPs showed diffusion controlled release. Bioavailability study of batch CS9 was studied in rabbit eye and compare to 5-FU solution. 5-FU level was significantly higher in aqueous humor of rabbit eye. Ocular tolerance was studied in the eye of New Zealand rabbits and tested formulation was non-irritant with no sign of inflammation. © 2011 Pharmaceutical Society of Japan.
CNT induced β-phase in polylactide: Unique crystallization, biodegradation, and biocompatibility
(2013) Narendra K. Singh; Sunil K. Singh; Debabrata Dash; Prasad Gonugunta; Manjusri Misra; Pralay Maiti
The effect of multi-walled carbon nanotube (MWCNT) on the crystal structure, unique crystallization, mechanical behavior, enzymatic degradation, and significant improvement in biocompatibility of polylactide (PLA) nanohybrid has been reported. Functionalization of carbon nanotube using stearyl alcohol has been carried out and has been confirmed through FTIR and Raman spectroscopy. PLA nanohybrids have been synthesized using functionalized and neat MWCNT through solution route, and the improved level of dispersion of MWCNT has been achieved in PLA matrix. High-magnification transmission electron microscope images indicate the unique adsorption of PLA chain leading to the crystallization of β-phase structure on the surface of the functionalized MWCNT against the usual crystallized α-form of pure PLA. The presence of β phase in nanohybrids has been confirmed through electron diffraction pattern, differential scanning calorimetry thermograms, and X-ray diffraction patterns. The improved and diverse mechanical, thermal properties, and crystallization kinetics have been explored with the special emphasis on the relaxation behavior of β phase in dynamic mechanical analysis. The cause of these developments has been appraised from the interaction point of view as calculated from the interaction parameter (χ) using melting-point depression technique. The rate of biodegradation has been studied in detail with plausible mechanism in Proteinase K enzyme media showing their specificity and tuning of biodegradation rate followed by their optimization. For biomedical applications, the effect of pure polymer and nanohybrids on circulating blood cells has been evaluated in detail, and the hemocompatible nature of the nanohybrids has been revealed, suppressing the cellular toxicity of MWCNT. © 2013 American Chemical Society.
Colorimetric detection of hydrogen peroxide and glucose using brominated graphene
(Royal Society of Chemistry, 2017) Shikha Singh; Kheyanath Mitra; Rajshree Singh; Archana Kumari; Susanta Kumar Sen Gupta; Nira Misra; Pralay Maiti; Biswajit Ray
Very recently, we have reported a novel peroxidase mimetic material, brominated graphene (GBR) having ∼3% bromine content, which, in combination with H2O2 and 3,3′,5,5′-tetramethylbenzidine (TMB), has shown the property of S2- ion recognition (Anal. Chem., 2017, 89, 783-791). In the present work, we further have investigated the kinetic assay and colorimetric sensing ability of GBR towards hydrogen peroxide (H2O2) and glucose. The Michaelis-Menten constants (Km) and maximum initial velocities (Vmax) of GBR have been found to be 10.98 mM and 3.60 × 10-8 M s-1, respectively, for H2O2 and 0.83 mM and 0.68 × 10-8 M s-1, respectively, for TMB. A sensor combining TMB and GBR has been fabricated, which, upon addition to H2O2 or glucose with glucose oxidase solution at pH 4.48, showed colorimetrically a significant increase in the oxidation of TMB. The fabricated sensor system has displayed linearity for H2O2 and glucose estimation in the range 0.50-5.00 mM and 40-100 mM, respectively, and the corresponding limits of detection are found to be 0.417 and 28.41 mM, respectively. The present sensor system is also highly reproducible and selective. The results of real samples using this colorimetric method have been found to be comparable with the conventional auto-analyser method. © The Royal Society of Chemistry 2017.
Conducting nano-channels in an induced piezoelectric polymeric matrix using swift heavy ions and subsequent functionalization
(2012) Karun Kumar Jana; Biswajit Ray; Devesh K. Avasthi; Pralay Maiti
Nanohybrids of poly(vinylidene fluoride) with layered silicates have been synthesized by melt extrusion and nano-channels have been fabricated by swift heavy ion (SHI) irradiation followed by chemical etching of the selective amorphous zones in the latent tracks. The channel diameter was reduced to 30 nm in the presence of nanoclay in the nanohybrid, making it suitable for membrane applications. Grafting with styrene was carried out inside the nano-channels using the free radicals created by the SHI exposure. Sulphonation on the grafted polystyrene was carried out to increase the conductivity of the membrane to the semiconducting range through ion channel conduction. The grafting and sulphonation inside the nano-channels were confirmed through spectroscopic techniques viz. NMR, FTIR, UV and molecular weight measurements. The dimensions of the channels are controlled by the SHI fluence and thereby dictates the properties including 10 orders of magnitude higher conductivity by creating a greater number of channels and hence increasing the surface area required for enhanced grafting and sulphonation. The hetero-junction and nano-channel conduction was demonstrated through STM measurements showing that the superior conduction depends on the relative extent of grafting and sulphonation in pure PVDF and the nanohybrid. The matrix PVDF crystallizes in the piezoelectric β-phase in the presence of nanoclay and promotes the formation of smart membranes. © 2012 The Royal Society of Chemistry.
Conjugated polymers for solar cell applications
(Elsevier, 2022) Pravesh Kumar Yadav; Sunil Kumar; Pralay Maiti
Conjugated polymers (CPs) have become the focus of major research and development initiatives owing to their exceptional and tunable properties as well as their applicability in different fields. Further, solar cell technology represents an existing renewable energy technology that has attracted considerable attention in recent years. Today, it has become the most promising area to turn to with regards to worldwide energy issues. This is because it offers the possibility to minimize greenhouse gas emissions as well as the detrimental environmental effects of the ever increasing consumption of energy from nonrenewable sources worldwide in recent decades. Furthermore, CPs have tremendous potential advantages, such as renewability, low cost, light weight, greater flexibility, superior optical absorption, large area production, and easy processability. In this chapter, we provide an oversight of current achievements regarding the application of CPs in various classes of solar cells, which are here presented and analyzed considering different examples of active materials. The advantages of CPs and the challenges associated with their use in this regard are also introduced in this chapter. Additionally, we also discuss the basic fundamental concepts and techniques for CP synthesis as well as the basic working principle, device architecture, characterization, photovoltaic key parameters, and strategy for enhancing the performance of organic solar cells. The trump card of CPs in solar cell applications is the major focus of this chapter. © 2022 Elsevier Ltd. All rights reserved.
Controlled DNA Delivery Using Poly(lactide) Nanoparticles and Understanding the Binding Interactions
(American Chemical Society, 2021) Sudipta Senapati; Anurag Upadhyaya; Somnath Dhruw; Debaprasad Giri; Pralay Maiti
Cationic polymer-based gene delivery vectors suffer from several limitations such as low DNA-loading capacity, poor transfection, toxicity, environmental degradations, etc. Again, very limited works are available addressing the binding interactions in detail at the atomic level explaining the loading capacity, protection ability against harsh environments, and controlled release behavior of the DNA-encapsulated vehicles. Here, a poly(l-lactide) (PLA) nanoparticle-based controlled DNA release system is proposed. The developed vehicle possesses a high DNA-loading capacity and can release the loaded DNA in a controlled manner. Spectroscopic, physicochemical, and molecular simulation techniques (AM1 and atomistic molecular dynamics) have been employed to understand the binding interactions between PLA and DNA molecules enabling high DNA loading, protection against external harsh environments, and controlled DNA release behavior. Methyl thiazolyl tetrazolium (MTT) assay experiments confirm the biocompatible nature of the vehicle. Cellular uptake efficiency and endo-lysosomal escape capabilities have been investigated against HeLA cells. This study, therefore, demonstrates the development of a promising nonviral DNA delivery vector and includes a detailed investigation of the atomic-level interaction behavior between PLA and DNA molecules. © 2021 American Chemical Society
Controlled drug release through regulated biodegradation of poly(lactic acid) using inorganic salts
(Elsevier B.V., 2017) Sunil Kumar; Shikha Singh; Sudipta Senapati; Akhand Pratap Singh; Biswajit Ray; Pralay Maiti
Biodegradation rate of poly(lactic acid) (PLA) has been regulated, both increase and decrease with respect to the biodegradation of pure PLA, by embedding meager amount of inorganic salts in polymer matrix. Biodegradation is performed in enzyme medium on suspension and film and the extent of biodegradation is measured through spectroscopic technique which is also verified by weight loss measurement. Media pH has been controlled using trace amount of inorganic salt which eventually control the biodegradation of PLA. High performance liquid chromatography confirms the hydrolytic degradation of PLA to its monomer/oligomer. Induced pH by metal salts show maximum degradation at alkaline range (with calcium salt) while inhibition is observed in acidic medium (with iron salt). The pH of media changes the conformation of enzyme which in turn regulate the rate of biodegradation. Thermal degradation and increment of modulus indicate improvement in thermo-mechanical properties of PLA in presence of inorganic salts. Functional stability of enzyme with metal salts corresponding to acidic and alkaline pH has been established through a model to explain the conformational changes of the active sites of enzyme at varying pH influencing the rate of hydrolysis leading to regulated biodegradation of PLA. The tuned biodegradation has been applied for the controlled release of drug from the polymer matrix (both sustained and enhanced cumulative release as compared to pure polymer). The cell proliferation and adhesion are influenced by the acidic and basic nature of polymeric material tuned by two different inorganic salts showing better adhesion and proliferation in calcium based composite and, therefore, suggest biological use of these composites in biomedical applications. © 2017 Elsevier B.V.
Controlled release of drug and better bioavailability using poly(lactic acid-co-glycolic acid) nanoparticles
(Elsevier B.V., 2016) Sanjeev K. Pandey; Dinesh K. Patel; Akhilendra K. Maurya; Ravi Thakur; Durga P. Mishra; Manjula Vinayak; Chandana Haldar; Pralay Maiti
Tamoxifen (Tmx) embedded poly(lactic-co-glycolic acid) (PLGA) nanoparticles (PLGA-Tmx) is prepared to evaluate its better DNA cleavage potential, cytotoxicity using Dalton's lymphoma ascite (DLA) cells and MDA-MB231 breast cancer cells. PLGA-Tmx nanoparticles are prepared through emulsified nanoprecipitation technique with varying dimension of 17-30 nm by changing the concentrations of polymer, emulsifier and drug. Nanoparticles dimension are measured through electron and atomic force microscopy. Interactions between tamoxifen and PLGA are verified through spectroscopic and calorimetric methods. PLGA-Tmx shows excellent DNA cleavage potential as compared to pure Tmx raising better bioavailability. In vitro cytotoxicity studies indicate that PLGA-Tmx reduces DLA cells viability up to ~38% against ~15% in pure Tmx. Hoechst stain is used to detect apoptotic DLA cells through fluorescence imaging of nuclear fragmentation and condensation exhibiting significant increase of apoptosis (70%) in PLGA-Tmx vis-à-vis pure drug (58%). Enhanced DNA cleavage potential, nuclear fragmentation and condensation in apoptotic cells confirm greater bioavailability of PLGA-Tmx as compared to pure Tmx in terms of receptor mediated endocytosis. Hence, the sustained release kinetics of PLGA-Tmx nanoparticles shows much better anticancer efficacy through enhanced DNA cleavage potential and nuclear fragmentation and, thereby, reveal a novel vehicle for the treatment of cancer. © 2016 Elsevier B.V.
Corrigendum to “Controlled release of drug and better bioavailability using poly(lactic acid-co-glycolic acid) nanoparticles” [Int. J. Biol. Macromol. 89 (2016) 99–110] (S0141813016303737) (10.1016/j.ijbiomac.2016.04.065))
(Elsevier B.V., 2018) Sanjeev K. Pandey; Dinesh K. Patel; Akhilendra K. Maurya; Ravi Thakur; Durga P. Mishra; Manjula Vinayak; Chandana Haldar; Pralay Maiti
The authors regretfully submit that there was human mistake and it can be rectified by adding a corrigendum in which the following be mentioned: [Figure presented] Fig. 1a incorrect one be immediately replaced by the Fig. 1a correct one. The human mistake was due to folders having electron micrograph of all experiment and during transfer of images from technical person to us. The authors would like to apologies for inconvenience caused. © 2018 Elsevier B.V.
Corrigendum to “Layered double hydroxides as effective carrier for anticancer drugs and tailoring of release rate through interlayer anions” [Journal of Controlled Release 224 (2106) 186–198] (Layered double hydroxides as effective carrier for anticancer drugs and tailoring of release rate through interlayer anions (2016) 224 (186–198), (S016836591630013X), (10.1016/j.jconrel.2016.01.016))
(Elsevier B.V., 2021) Sudipta Senapati; Ravi Thakur; Shiv Prakash Verma; Shivali Duggal; Durga Prasad Mishra; Parimal Das; T. Shripathi; Mohan Kumar; Dipak Rana; Pralay Maiti
The authors regret that the initial published version of this article an error in the assembly of Fig. 7b resulted in some image duplications. The corrected Fig. 7b includes the correct images of the experiment. This correction/omission doesn't alter any conclusion of the article as quantitative analysis of the experiment has been performed through MTT assay, presented in Fig. 7a. [Figure Presented] The figure legend remains the same. The corrections made in this corrigendum do not affect the original conclusions of the article. The author's apologies for any inconvenience caused. © 2016 Elsevier B.V.
Development of ciprofloxacin hydrochloride loaded poly(ethylene glycol)/chitosan scaffold as wound dressing
(2013) Mukty Sinha; Rathindra M. Banik; Chandana Haldar; Pralay Maiti
A novel ciprofloxacin hydrochloride loaded chitosan/poly(ethylene glycol) (PEG) composite scaffold was developed for wound dressing application. PEG incorporation in chitosan scaffold showed enhanced loading up to 5.4 % and increased cumulative release of the drug up to 35 % as compared to pure chitosan scaffold (20 %). The drug loading and control release of the drug has been explained by the morphological features and drug-polymer/polymer-polymer interactions revealed by SEM, FTIR and DSC. Bacterial growth inhibition evaluation using Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus confirmed the efficacy of released drug from the scaffolds (pure and PEG mixed chitosan). Swelling study, bacterial penetration, moisture vapour transmission rate, haematocompatibility and biodegradation profile supported the suitability of scaffold used as wound dressing materials. In-vivo study on mice finally validated the controlled rate of drug release showing the effectiveness of PEG incorporation into the scaffold for quicker and regulated wound healing. © 2012 Springer Science+Business Media New York.
Dextrin and polyurethane graft copolymers as drug carrier: Synthesis, characterization, drug release, biocompatibility and in-vitro toxicity
(Elsevier Ltd, 2021) Aparna Shukla; Swapan Maity; Biswajit Ray; Pralay Maiti
Chemical modification of dextrin with polyurethane as a graft has been synthesized for controlled drug release for longer time period by maintaining the hydrophobic–hydrophilic balance. Estimation of the degree of grafting is visualized from the integrated peak area in the NMR spectra. Particle nature of dextrin is converted into strip like morphology in polyurethane graft dextrin copolymers as obvious from atomic force microscopy. Drug release study of graft copolymers through in vitro studies indicates sustained drug release behavior as compared to pristine dextrin and specific interactions between polymer and drug have been verified through spectroscopic techniques. Biocompatibility of the graft copolymers has been revealed using cellular studies on cancerous HeLa cells through MTT assay and cell adhesion. Further, the cytotoxicity or the cell killing efficiency has been demonstrated resulting in significant cell mortality using the developed graft copolymers. © 2021